4种病原菌特异基因片段阳性蜱的16S rRNA基因克隆文库分析

Objective To develop the method of 16S rRNA gene clone library for tick bacterial flora analysis, and to analyze the detection effective of pathogens in tick and capacity of bacterial flora diversity. Methods Primers were designed according to the specific gene of Borrelia burgdorferi, Bartonella henselae, Anaplasma phagocytophilum, Ehrlichia chaffeensis and templates were choosen by positive PCR result to amplify the DNA extracted from the ticks. One set of primers targeting 16S rRNA gene conserved region were chosen to amplify certain fragments, DNA extraction, PCR reaction, cloning and sequencing. Nucleotide sequences were compared with GenBank database. Calculated Coverage values of clone library and Shannon-Wiener diversity index. Results Sixteen defined genus-or species-bacteria were detected in 103 valid sequences. Eight species were edge type (Clone No. > 5). Three kinds of pathogens were identified (Borrelia burgdorferi, Bartonella henselae and Rickettsia sp). Three kinds of pathogens were not edge type(Clone No. < 5). Coverage value was 96.11%, and Shannon-Wiener index was 2.40. Analysis results of cloning sequence showed that tick-parasitic bacteria mainly were α and γ deformation mycetes which accounted for 56.25% (9/16). Conclusions The 16S rRNA gene sequences technology could make relative quantitative of bacterial flora, and detect many kinds of pathogens in tick. It's a good method for detection of pathogens and bacterial flora analysis.     

4种病原菌特异基因片段阳性蜱的16S rRNA基因克隆文库分析

Objective To develop the method of 16S rRNA gene clone library for tick bacterial flora analysis, and to analyze the detection effective of pathogens in tick and capacity of bacterial flora diversity. Methods Primers were designed according to the specific gene of Borrelia burgdorferi, Bartonella henselae, Anaplasma phagocytophilum, Ehrlichia chaffeensis and templates were choosen by positive PCR result to amplify the DNA extracted from the ticks. One set of primers targeting 16S rRNA gene conserved region were chosen to amplify certain fragments, DNA extraction, PCR reaction, cloning and sequencing. Nucleotide sequences were compared with GenBank database. Calculated Coverage values of clone library and Shannon-Wiener diversity index. Results Sixteen defined genus-or species-bacteria were detected in 103 valid sequences. Eight species were edge type (Clone No. > 5). Three kinds of pathogens were identified (Borrelia burgdorferi, Bartonella henselae and Rickettsia sp). Three kinds of pathogens were not edge type(Clone No. < 5). Coverage value was 96.11%, and Shannon-Wiener index was 2.40. Analysis results of cloning sequence showed that tick-parasitic bacteria mainly were α and γ deformation mycetes which accounted for 56.25% (9/16). Conclusions The 16S rRNA gene sequences technology could make relative quantitative of bacterial flora, and detect many kinds of pathogens in tick. It's a good method for detection of pathogens and bacterial flora analysis.     

医务人员主动提供艾滋病检测咨询效果分析

目的分析宁夏开展医务人员主动提供艾滋病检测咨询工作实施效果,为调整艾滋病防治措施提供依据。方法收集2010-2016年全区艾滋病筛查实验室及检测点报告的艾滋病检测份数表和2010-2016年报告HIV/AIDS病例,采用SPSS18.0进行统计分析。结果 2010-2016年累计HIV抗体筛查检测43万人次,确证阳性数从63例增加到1639例。其中术前筛查占总筛查人数比例最多为29.9%、孕产期检测14.4%、其他门诊就诊筛查11.2%;通过术前检测发现阳性数占比最高为22.1%、其他就诊者检测15.3%、性病门诊就诊者检测10.9%;术前检测检出阳性率、性病门诊筛查检出阳性率和、自愿咨询检测检出阳性率均呈上升趋势。历年晚发现比例下降不明显。结论医疗机构开展扩大检测,检测人数逐年增加,发现阳性病例数逐年增加,检测人数占常住人口比例逐年上升,检测发现阳性率呈上升趋势,应持续在医疗机构重点科室开展艾滋病筛查检测工作。     

马凤琴应用健脾清热祛湿法治疗慢性胃炎伴糜烂经验

马凤琴老师认为,慢性胃炎伴糜烂是以脾虚为根本,湿热为其标,日久则会引起血瘀,辨证过程中格外注意患者的舌象,根据舌象把握患者病情变化发展,在治疗中当以健脾、清热、祛湿为主,佐以活血化瘀法,运用自拟方芩连平胃散加减辨证治疗慢性胃炎伴糜烂有良好效果。马凤琴老师认为,慢性胃炎伴糜烂在疾病康复中调养大于治疗,所以在辨证选方用药的同时,也需要调整患者的饮食习惯,保持乐观的心态,这对于改善慢性胃炎伴糜烂所引起的胀痛、泛酸、嗳气等不适有重要作用。     

自制简易胃肠减压器

胃肠道手术,都离不开胃肠减压,以前我们用20ml 注射器抽胃液。压力大小不易掌握,有时把胃管抽扁,有时损伤胃粘膜,又达不到持续减压之目的。为此,采用自制胃肠减压器,经临床500例病人使用观察,效果满意。负压、压力大小易掌握,不损伤胃粘膜,又能达到持续减压之目的。用后将瓶子冲洗干净,再用0.4%过氧乙酸溶液浸泡     

对我院住院病人优质服务观点的动态调查及分析

目的探讨近几年我院住院病人对优质服务的观点及其变化.方法自行设计问卷,分别于1995年、1998年、2002年和2004年对我院住院病人进行调查.统计调查结果,并将住院病人对优质服务的观点进行纵向分析类比.结果我院病人在住院期间对优质服务的观点可归纳为4个方面服务态度好、业务精湛、讲解疾病相关知识、对病人实施人文关怀.结论病人对优质服务的观点在近年逐渐发生转变,临床护理应加强人文关怀.     

非急性期脑血管病患者血糖及相关因素的临床研究

目的分析研究非急性期脑血管病患者血糖及其相关因素的临床意义。方法以43例非急性期脑血管病患者（A组）及35例正常体检者（B组）为研究对象,两组（其中A组于发病2周后）均行糖耐量试验,测定空腹胰岛素（FINS）,测量身高、体重、腰围,计算体重指数（BMI）及胰岛素抵抗指数（HOMA-IR）,比较两组间的数据。结果 A组体重、BMI、腰围和FBG均较B组高,有统计学意义（P〈0.05）,餐后两小时血糖（PBG）、HOMA值、LnInS值明显高于B组（P〈0.001）。结论高血糖是非急性期脑血管病患者普遍存在的危险因素,其与动脉粥样硬化的其它危险因素共同作用导致发病。     

骨折合并糖尿病患者的围手术期综合护理体会

目的探讨骨折合并糖尿病患者围手术期的综合护理措施。方法回顾性分析48例骨折合并糖尿病患者围手术期的综合护理措施。结果48例患者采取围手术期的综合护理措施后,除3例患者（占6．25％）切口出现感染,经换药处理后愈合外。其他45例患者（占93．75％）手术切口均甲级愈合,如期拆线。所有患者骨折端生长良好,无骨不连等并发症发生。结论对骨折合并糖尿病患者的围手术期采取综合护理措施（血糖监测护理、心理护理、饮食护理、预防感染及并发症护理、功能锻炼护理等）,是减轻患者痛苦、减少并发症、提高临床疗效的可靠有效手段,有利于患者的骨折愈合和身体的早日康复。     

甘草油联合湿疹软膏及护理干预在婴幼儿湿疹中的应用

目的:总结甘草油联合湿疹软膏及护理干预在婴幼儿湿疹中的治疗、预防和护理体会。方法:结合近年来我院采用甘草油与湿疹软膏治疗的200例婴幼儿湿疹病例,对湿疹的治疗、护理及预防措施进行综合评述。结果:甘草油与湿疹软膏治疗婴幼儿湿疹有效率达98.5%。正确的护理方法可以减少婴幼儿湿疹的发生率,加强家庭日常护理和喂养是预防婴幼儿湿疹的重要措施。结论:可采用甘草油联合湿疹软膏治疗婴幼儿湿疹,同时加强日常护理,可减轻症状,减少复发。     

米非司酮配伍米索前列醇终止5～16周妊娠的临床结果分析

米非司酮配伍米索前列醇终止早期妊娠已在全国广泛应用 ,由于痛苦小、简便 ,选择药物流产者众多 ,本院利用米非司酮抗早孕和米索前列醇促子宫收缩的原理 ,开展米非司酮配伍米索前列醇终止 5～ 16周妊娠 ,现将本院从 1998～ 2 0 0 1年 10 6 0例药物流产临床结果分析如下。1　资料与方法1 1　对象　经门诊确认为宫内孕 5～ 16周 ,年龄在 2 0～ 4 2周岁 ,自愿要求药物流产 10 6 0例健康妇女 ,交待药物流产的机理及注意事项 ,无药物过敏史 ,流产前检查血、尿、粪常规及肝肾功能 ,心电图正常 ,B超确认为宫内妊娠 ,药物流产对象留院观察 6～ 10h …