中药熏蒸辅助治疗对重度类风湿膝关节炎人工膝关节置换术后患者康复效果的影响

目的:探讨中药熏蒸辅助治疗对重度类风湿膝关节炎人工膝关节置换术后患者康复效果。方法:选取我院骨科2017年1月至2018年2月收治的重度类风湿膝关节炎需行人工膝关节置换术患者83例。按就诊先后顺序分为试验组和对照组,试验组40例,对照组43例。对照组患者常规康复功能锻炼,试验组患者在对照组的基础上给予中药熏蒸辅助治疗,治疗3个月。对比两组患者治疗治疗前和治疗后后血清因子水平,治疗前后生活质量评分量表(SF-36)评分,两组患者骨关节炎指数评分(The Western Ontario and Mcmaster Universities Osteoarthritis Index,WOMAC)、膝关节评分(Hospital for special surgery,HSS)。结果:经治疗治疗前和治疗后后试验组血浆纤维蛋白(FIB)、D-二聚体、IL-1β、白细胞介素8(IL-8)水平均明显降低(P<0.05);试验组SF-36量表评分显著高于对照组(P<0.05);试验组WOMAC评分、HSS评分显著高于对照组(P<0.05)。结论:中药熏蒸辅助治疗能有效提高重度类风湿膝关节炎人工膝关节置换术后患者康复效果。     

艾司唑仑联合松静诱导训练对腰椎间盘突出症术后患者睡眠质量及认知的影响

目的 探讨腰椎间盘突出症术后患者给予松静诱导训练联合艾司唑仑治疗后患者认知及睡眠质量变化.方法 将本院122例腰椎间盘突出症术后患者通过掷币法分为松静诱导组61例及联合药物组61例,松静诱导组患者术后指导患者进行松静诱导训练,联合药物组患者术后增加艾司唑仑口服治疗,对比两组患者治疗后心理状态、睡眠质量及认知功能变化.结...     

独活寄生汤加味联合膝四针治疗风寒湿痹型膝骨关节炎临床研究

目的：观察独活寄生汤联合膝四针治疗风寒湿痹型膝骨关节炎的临床疗效。方法：将90 例风寒湿痹型膝骨关节炎患者按随机数字表法分为观察组与对照组各45 例,对照组口服硫酸氨基葡萄糖胶囊治疗,观察组口服独活寄生汤联合膝四针治疗。比较2 组临床疗效,比较2 组治疗前后视觉模拟评分法（VAS） 评分、压痛值、西安大略和麦克马斯特大学骨关节炎指数评分（WOMAC）、中医证候积分、血清疼痛介质5-羟色胺（5-HT）、前列腺素E2（PGE2）、多巴胺（DA）、P 物质（SP） 及炎症介质肿瘤坏死因子-α （TNF-α）、白细胞介素-1 （IL-1）、白细胞介素-6 （IL-6） 水平。结果： 观察组总有效率88.89%, 高于对照组77.78% （P＜0.05）。与同组治疗前比较,治疗后2 组VAS 评分、WOMAC 各项评分、各项中医证候积分及PGE2、SP、DA、5-HT、IL-1、IL-6、TNF-α 水平均降低（P＜0.05）,压痛值均升高（P＜0.05）;与对照组治疗后比较,观察组治疗后VAS 评分、WOMAC 各项评分、各项中医证候积分及PGE2、SP、DA、5-HT、IL-1、IL-6、TNF-α 水平均较低（P＜0.05）,压痛值较高（P＜0.05）。结论：独活寄生汤联合膝四针治疗风寒湿痹型膝骨关节炎临床疗效较好,可有效缓解患者疼痛,其作用机制可能与降低血清疼痛介质及炎症介质有关。     

温电针齐刺环跳穴辅治腰椎间盘突出坐骨神经痛临床观察

目的:观察温电针齐刺环跳穴治疗腰椎间盘突出坐骨神经痛对疼痛程度、血流变指标及血清炎性因子水平的影响.方法:97例采用抽签法分为对照组48例及观察组49例,两组均给予膀胱经下肢腧穴针刺治疗,观察组联合温电针齐刺环跳穴.结果:治疗后观察组疼痛分级指数(PRI)、疼痛视觉模拟量表(VAS)、现时疼痛强度(PPI)评分均小于对...     

益气活络汤熏蒸加针刺联合西药对脑梗死后肩-手综合征疗效、肩关节疼痛程度、偏瘫侧上肢运动功能及痉挛评分的影响

目的：观察益气活络汤熏蒸加针刺联合西药治疗脑梗死后肩-手综合征的临床疗效。方法：选取郑州大学第二附属医院南阳路院区康复科收治的78例脑梗死后肩-手综合征患者,按1∶1的比例分为两组。对照组给予丹参川芎嗪注射液(由贵州拜特制药有限公司生产,批号20200612,5 mL/支),每隔3 d注射1次;针刺患侧肩髃、肩髎、肩贞、曲池、臂臑、手三里、合谷、外关、后溪及头枕等,行针1 min后留针30 min, 1 d治疗1次,每周针刺5次。治疗组在对照组治疗基础上给予益气活络汤(黄芪、黄精、制首乌、当归、川芎、赤芍、僵蚕、全蝎)熏蒸,1 d 2次。两组均持续治疗4周后判定疗效。结果：治疗组治愈13例,好转23例,无效3例,有效率为92.31%(36/39);对照组治愈9例,好转17例,无效13例,有效率为66.67%(26/39)。两组对比,差异有统计学意义(P<0.05)。在肩关节疼痛程度、偏瘫侧上肢运动功能、痉挛评分方面对比,治疗组治疗后优于对照组(P<0.01)。结论：益气活络汤熏蒸加针刺联合西药治疗脑梗死后肩-手综合征疗效确切。     

Noth2调控舌鳞癌干细胞的增殖、侵袭和迁移

BACKGROUND:Previous studies have found that the expression of Notch2 is up-regulated in tongue squamous cell carcinoma (TSCC) stem cells, but the effect of Notch2 on TSCC remains unclear. OBJECTIVE:To explore the effect of Notch2 on the proliferation, invasion and migration of TSCC stem cells (ALDH^br). METHODS:ALDHbr cells were enriched and separated by fluorescence-activated cell sorting technique from Tca8113 cells cultured in using serum-free medium, and Notch2 expression in ordinary TSCC cells (ALDH^low) and ALDH^br cells was detected by western blot. The ALDH^br cells transfected with Notch2 shRNA (sh-Notch2) by liposome served as experimental group, and ALDH^br cells transfected with sh-control were as control group. The effects of Notch2 on the proliferation, invasion and migration of ALDH^br cells were detected by cell counting kit-8 kit and Transwell assay, respectively. In addition, the expression levels of Ki67, matrix metalloproteinases 2 and 9 protein in Notch2-silenced ALDH^br cells and control cells were detected by western blot. RESULTS AND CONCLUSION:The expression level of Notch2 in ALDH^br cells was significantly higher than that in ALDH^low cells (P < 0.05). The ALDHbr cell proliferation ability and Ki67 protein expression in the experimental group were obviously lower than those in the control group (P < 0.05). The ALDH^br cell invasion and migration abilities in the experimental group were significantly lower than those in the control group (P < 0.05). The expression levels of matrix metalloproteinases 2 and 9 protein in the experimental group were obviously lower than those in the control group (P < 0.05). All these results show that Notch2 can promote the proliferation, invasion and migration of ALDH^br cells, which suggests that Notch2 plays an important role in the occurrence and development of TSCC, and it may be a potential molecular target for the treatment of TSCC.