聚合酶链反应诊断女性外阴尖锐湿疣初步探讨

聚合酶链反应诊断女性外阴尖锐湿疣初步探讨郑惠方单祥年李明发蒋清龚嘉仪（南京铁道医学院附属医院妇产科，生物学教研室，附属医院病理科南京２１０００９）尖锐湿疣（ＣＡ）主要由人乳头瘤病毒（ＨＰＶ）６及１１型ＤＮＡ感染引起。尖锐湿疣和假性湿疣...     

DHT对Hep—2细胞的影响

本文采用中性红摄入法检测不同浓度的雄性激素－双氢睾酮（ＤＨＴ）对体外培养人喉鳞状细胞细胞系（Ｈｅｐ－２）影响以及对喉癌细胞的作用。结果发现１０＾－１３ｍｏｌ的ＤＨＴ即可促使喉癌细胞增殖，表明雄性激可促进喉癌细胞的生长，这为喉癌的性激素治疗提供了理论根据。     

健康中老年人与恶性肿瘤病人血清脂质结合唾液酸含量的研究

本文采用改良的间苯二酚法对129例中老年病人(良性疾病40例,恶性肿瘤89例)及60例健康对照者的血清脂质结合唾液酸含量进行了研究.测定结果表明,60例健康对照者为0.136±0.031g/L,特异性98.3％;40例良性疾病者为0.157±0.034g/L,特异性95％;89例恶性肿瘤病人为0.245±0.08g/L,总阳性率为74.1％,其中肺癌、胃癌、喉舌及鼻窦癌和白血病的阳性率较高。     

类固醇糖尿病的免疫组化研究

应用免疫组织化学方法，研究了类固醇糖尿病胰岛内分泌细胞量的变化。结果表明类固醇糖尿病产生胰岛素的细胞明显减少，而其他胰岛细胞无明显变化     

海马中一氧化氮通过海马—NPY—PAA调节细胞免疫功能

研究发现隔－海马通路损伤后，海马中一氧化氮降低同时大鼠细胞免疫功能增强。为此，观察了海马中神经肽Ｙ（ＮＰＹ）和血浆中促肾上腺皮质激素（ＡＣＴＨ）和皮质酮（ＣＮ）的变化，结果提示海马中一氧化氮伴随海马－ＮＰＹ－垂体－肾上腺轴的变化而影响大鼠的细胞免疫功能。     

腺病毒重组质粒作为免疫PCR指示分子的研究

为探讨不同标记方式对制备免疫PCR指示分子的影响,首先制备了一个用于免疫PCR指示分子DNA片段的重组质粒（腺病毒六邻体基因片段）,用生物素-dUTP分别以不同的方式标记DNA:PCR产物标记、PCR直接标记、线性重级质粒标记和回收重组质粒插入片段的标记。结果表明,以线性重组质粒标记生物素制备的指示分子具有最佳的效果。     

Flow-induced Expression and Phosphorylation of VASPin Endothelial Cells

1 Introduction It is well known that mechanical forces have important influence on endothelial cells, in particular, on cytoskeleton reorganization. VASP (vasodilator stimulated phosphoprotein) is a 46 KD actin associated protein. It is a member of Ena/VASP protein family and composed of EVH1, proline-rich and EVH2 domains. It is considered as an important component of the sub-cellular regions where remodelling of the actin cytoskeleton takes place, such as the front of spreading lamell…     

肺炎患儿血清sIL—2Rα水平变化的初步研究

可溶性白细胞介素2受体α链(sIL-2Rα)在血清及其他体液中的变化与许多疾病有关,一般认为它主要与膜白细胞介素2受体(mIL-2R)竞争结合白细胞介素2(IL-2)而起抑制免疫功能的作用。肺     

一种简便快速回收DNA的试剂盒研制

多年来已提出过许多从凝胶中回收DNA的方法[1],但没有一种方法能够完全满足进一步实验的要求.目前国内多使用进口DNA回收试剂盒,但价格昂贵.我们通过对进口DNA回收试剂盒的分析研究,采用国产玻璃毛为DNA载体,依据化学键与分子结构理论及分子运动规律,结合DNA分子的特性,研制出一种简便、快速、高质量、低成本的DNA回收试剂盒,所回收的DNA量大、纯度高,可以用于分子生物学等的各种实验.     

Chronic neuroprotective effects of low concentration lithium on SH-SY5Y cells:possible involvement of stress proteins and gene expression

To investigate the molecular mechanism underlying the neuroprotective effect of lithium on cells, in this study, we exposed SH-SY5Y cells to 0.5 mmol/L lithium carbonate(Li2CO2) for 25–50 weeks and then detected the expression levels of some neurobiology related genes and post-translational modifications of stress proteins in SH-SY5Y cells. cDNA arrays showed that pyruvate kinase 2(PKM2) and calmodulin 3(CaM 3) expression levels were significantly down-regulated, phosphatase protein PP2A expression was lightly down-regulated, and casein kinase II(CK2), threonine/tyrosine phosphatase 7(PYST2), and dopamine beta-hydroxylase(DBH) expression levels were significantly up-regulated. Besides, western blot analysis of stress proteins(HSP27, HSP70, GRP78 and GRP94) showed an over-expression of two proteins: a 105 kDa protein which is a hyper-phosphorylated isoform of GRP94, and a 108 kDa protein which is a phosphorylated tetramer of HSP27. These results suggest that the neuroprotective effects of lithium are likely related to gene expressions and post-translational modifications of proteins cited above.