参加肌酸激酶参考方法国际室间比对计划结果分析

目的 对2006-2008年3年来参加IFCC组织的参考实验室RELA的CK的测定结果 进行比较和分析,为我国CK参考方法 的建立和应用提供借鉴.方法 按照IFCC公布的酶学活性测定(37℃)参考方法 的标准操作程序(SOP)测定CK国际样本,不精密度评价按美国CLSI的EP5-A2进行,准确度评价采用国际有证参考物质(ERM).结果 2006年测定结果 ,样本A CK值为(9.896±0.112)μkat/L,样本B为(4.953±0.120)μkat/L;2007年测定结果 ,样本A为(2.684 ±0.054)μkat/L,样本B为(8.798 ±0.101)μkat/L;2008年测定结果 ,样本A为(10.523±0.149)μkat/L,样本B为(10.551±0.141)μkat/L.2006-2008年CK参考方法 的不精密度分别为0.92%、0.86%、0.88%,均＜1%,ERM测定均值在"靶值±不确定度[(1.68±0.07)μkat/L]"范围内,初步验证了参考方法 的不精密度和准确度.结论 连续3年的CK国际样本测定结果 均在IFCC公布的置信区间(limit of equivalence)±5%范围内,CK的IFCC参考方法 已经建立并且日趋成熟.     

刺血疗法治疗消化性溃疡疗效的临床研究

目的:研究临床上刺血疗法对消化性溃疡的治疗效果。方法:选取符合消化性溃疡诊断的患者32例,并进行刺血疗法治疗,观察疗效。结果:经过治疗,刺血疗法的总有效率为90.63%。结论:刺血疗法对治疗消化性溃疡具有较显著的临床疗效。     

血浆(1-3)-β-D-葡聚糖检测与真菌培养在侵袭性真菌感染诊断中的应用

目的 评价血浆(1-3)-β-D-葡聚糖检测和真菌培养在临床侵袭性真菌感染诊断中的意义.方法 回顾性分析138例疑似侵袭性真菌感染住院患者的血浆(1-3)-β-D-葡聚糖检测结果以及不同部位真菌培养结果,分别计算两种方法及联合检测的灵敏度、特异度、阳性和阴性预测值.结果 138例疑似侵袭性真菌感染的患者中确诊患者35例,血培养阳性仅1例;血浆(1-3)-β-D-葡聚糖检测的灵敏度、特异性、阳性和阴性预测值分别为74.3％、84.5％、61.9％、90.6％;真菌培养计数＞105 CFU/ml或＞2个部位同时培养阳性后,其灵敏度、特异性、阳性和阴性预测值分别为65.7％、99.0％、95.8％、89.5％;真菌培养联合血浆(1-3)-β-D-葡聚糖检测时,灵敏度、特异性、阳性和阴性预测值分别为45.7％、100.0％、100.0％、84.4％;血浆(1-3)-β-D-葡聚糖检测的阴性预测值较好,与真菌培养联合应用后阳性预测值高.结论 真菌培养计数＞ 105 CFU/ml或＞2个部位同时阳性时,以及血浆(1-3)-β-D-葡聚糖检测和真菌培养联合检测时可提高诊断的阳性预测值,减少假阳性的发生.     

参加肌酸激酶参考方法国际室间比对计划结果分析

ObjectiveTo provide a reference for the establishment and appliance of enzyme reference measurement of CK in China by comparing and analyzing the RELA results of CK in IFCC from 2006-2008. Methods The RELA samples of CK were measured according to the reference procedure for the measurement of catalytic activity concentration of CK (37 ℃) ,which had been published by IFCC. The EP5A2 protocol was used for evaluation of the imprecision and ERM was used for verification of the trueness. Results In RELA 2006, the result of sample A was (9. 896 ±0. 112) μkat/L, and the result of sample B was (4.953 ±0. 120) μkat/L. In RELA 2007, the result of sample A was (2.684 ±0.054)μkat/L, and the result of sample B was (8.798 μ0. 101) μkat/L. In RELA 2008, the result of sample A was (10. 523 ±0. 149) μkat/L,and the result of sample B was (10. 551 ±0. 141) μkat/L. The precision of the CK reference method in the year 2006 to 2008 was 0. 92%, 0. 86% and 0. 88% respectively, each of them is less than 1% and the results of ERMs were consistent with the certified value(1. 68 ± 0. 07)μkat/L,which verify the imprecision and accuracy of the reference method. Conclusions All of the results in the continuous three years were in the range of equivalence limits suggested by IFCC. The CK reference method suggested by IFCC has been established and it is getting better.     

cDNA基因芯片分析人心肌细胞能量代谢相关基因

目的通过对正常成年人心肌细胞mRNA表达差异性的分析，探讨心肌细胞能量代谢相关基因的变化，为今后全方面的研究和比较心肌的能量代谢的基因特点及变化趋势提供对比参数、为实现较早的对心肌细胞功能状态进行评价。方法将人心肌细胞相关的3368个cDNA制备成基因芯片，应用PCR、测序、荧光探针标记、双重杂交等方法，研究正常成人心肌细胞RNA与正常成人多组织参考RNA比较的基因差异表达。结果对成人已知心肌细胞3268个基因进行功能分组分析，得到高于成人多组织RNA对照1．5倍的基因576个，低于成人多组织RNA对照1．5倍的基因有1199个。与细胞能量代谢相关的基因有194个，其中有17个高表达，4个低表达；与细胞骨架和动力相关的基因有65个，其中有18个高表达，1个低表达。结论正常成人心肌细胞中存在许多相对于成人多组织RNA的特异标识基因，可为研究心血管疾病不同阶段不同病理状态提供客观参考体系。     

酶联免疫吸附试验检测乙型肝炎病毒血清标志物五项的结果模式分析

随着免疫技术的不断进步,检测乙型肝炎病毒标志物的方法越来越多,化学发光法( chemiluminescent microparticle immunoassay,CMIA)在临床检测中的地位越来越突出.酶联免疫吸附试验( enzyme linked immunosorbent assay,ELISA)由于灵敏度和特异性较高,测定下限进口试剂可达0.2 ng/ml,国产试剂目前也能达到0.5 ng/ml[1].并且酶联免疫吸附试验操作简单、方便、快速,适合批量操作,试验的成本低,只需要一台酶标仪、一台洗板机,一把可调节的定量加样枪,基本上就能够满足试验的要求,因此已经被临床和患者接受.     

γ-谷氨酰基转移酶国际室间比对计划结果分析

目的对2006～2008年参加国际临床化学联合会（IFCC）组织的参考实验室国际比对计划（RELA）的γ-谷氨酰基转移酶（GGT）测定结果进行比较和分析,为GGT参考方法的建立和应用提供借鉴。方法按照IFCC公布的酶学活性测定（37℃）参考方法的标准操作程序（SOP）测定GGT国际样本,不精密度评价按美国临床实验室标准化委员会（NCCLS）EP5-A2文件进行,准确度评价采用国际有证参考物质（ERM）。结果 2006年测定结果：样本A为（1.829±0.021）μkat/L,样本B为（3.331±0.019）μkat/L;2007年测定结果：样本A为（1.533±0.017）μkat/L,样本B为（3.840±0.035）μkat/L;2008年测定结果：样本A为（3.392±0.024）μkat/L,样本B为（3.058±0.024）μkat/L。2006～2008年GGT参考方法的不精密度分别为1.29%、0.44%、0.65%,ERM测定均值在＂靶值±不确定度＂[（1.90±0.04）μkat/L]范围内,初步验证了参考方法的不精密度和准确度。结论 2006～2008年RELA样本GGT测定结果均在IFCC公布的可信区间内,GGT的IFCC参考方法已经建立。     

参加肌酸激酶参考方法国际室间比对计划结果分析

ObjectiveTo provide a reference for the establishment and appliance of enzyme reference measurement of CK in China by comparing and analyzing the RELA results of CK in IFCC from 2006-2008. Methods The RELA samples of CK were measured according to the reference procedure for the measurement of catalytic activity concentration of CK (37 ℃) ,which had been published by IFCC. The EP5A2 protocol was used for evaluation of the imprecision and ERM was used for verification of the trueness. Results In RELA 2006, the result of sample A was (9. 896 ±0. 112) μkat/L, and the result of sample B was (4.953 ±0. 120) μkat/L. In RELA 2007, the result of sample A was (2.684 ±0.054)μkat/L, and the result of sample B was (8.798 μ0. 101) μkat/L. In RELA 2008, the result of sample A was (10. 523 ±0. 149) μkat/L,and the result of sample B was (10. 551 ±0. 141) μkat/L. The precision of the CK reference method in the year 2006 to 2008 was 0. 92%, 0. 86% and 0. 88% respectively, each of them is less than 1% and the results of ERMs were consistent with the certified value(1. 68 ± 0. 07)μkat/L,which verify the imprecision and accuracy of the reference method. Conclusions All of the results in the continuous three years were in the range of equivalence limits suggested by IFCC. The CK reference method suggested by IFCC has been established and it is getting better.