低温等离子体对血压计袖带的消毒效果实验研究

等离子体灭菌技术是消毒学领域继甲醛、环氧乙烷、戊二醛等低温灭菌技术之后又一新的低温灭菌技术,其特点是低温、快速灭菌、毒性残留低,而且对于耐湿热和不耐湿热的医疗器械均适用[1].由于血压计袖带在医院使用频繁,并且是反复使用,极易被病人体表携带的微生物所污染,故对血压计袖带的消毒已引起医院的重视[2].而目前医院内对血压计袖带多采用化学消毒液擦拭、紫外线照射或甲醛熏蒸等方法消毒.以上消毒方法有的有较多的毒性残留或有刺激性气味,有的需要较长的消毒时间,而低温等离子体灭菌方法可克服上述缺点.本实验研究采用高压电源为激发源.氩气为基础气体.介质阻挡放电(DBD)的方法产生大气压低温等离子体,对血压计袖带进行消毒,以观察其对血压计袖带表面自然菌的灭活效果.并与紫外线消毒血压计袖带进行比较.现报告如下.     

低温等离子体对细菌芽孢的灭活研究

目的 探讨低温等离子体对细菌芽孢的灭活效果及相关机制.方法 采用介质阻挡放电产生大气压低温等离子体,对枯草杆菌黑色变种芽孢进行处理,设定3个放电电极间距和7个处理时间,采用平板记数法记数存活芽孢数.并计算杀灭对数值(KLV).另外,研究高压电场灭菌效果并对低温等离子体处理后的枯草杆菌黑色变种芽孢进行透射电镜观察,用以初步探讨低温等离子体对细菌芽孢的灭活机制.结果 在电极间距分别为3、4和5 mm时.低温等离子体对枯草杆菌黑色变种芽孢的KLV超过5时的处理时间依次为25、30、35 s.机制研究发现.低温等离子体中的高压电场效应对枯草杆菌黑色变种芽孢的杀灭效果很差:透射电镜结果显示,低温等离子体对枯草杆菌黑色变种芽孢表面和内部结构都有损伤作用.结论 低温等离子体对细菌芽孢有良好的杀灭效果,且具有时间剂量依赖性.带电粒子的击穿作用和活性氧的氧化作用可能在低温等离子体杀灭芽孢的过程中起主要作用.而低温等离子体中的高压电场效应可以忽略不计.

Abstract：

Objective To investigate the effect of low-temperature plasma on inactivation of bacterial spores and explore the mechanism. Methods Dielectric barrier discharge (DBD) was employed to generate the atmospheric low-temperature plasma for treatment of B.subtilis var. niger spores with the gas spacing of 3,4 and 5 and treatment time intervals of 5, 10, 15, 20, 25, 30 and 35 s. The survived colonies was counted with plate counting method, and the killing log value (KLV) at different treatment times was calculated. The inactivation effect of electric field on B.subtilis var.niger spores was also investigated and the spores treated with low-temperature plasma were observed with transmission electron microscope. Results With the gap spacing of 3, 4 and 5 mm, the KLV of low-temperature plasma on B.subtUis var.niger spores within 25, 30 and 35 s of exposure was more than 5. The germicidal effects of the electric field on B. subtilis var.niger spores were rather poor. Transmission electron microscopy demonstrated total destruction of the surface and interior structure of the spores by low-temperature plasma. Conclusions Low-temperature plasma is effective for inactivation of the bacterial spores with a time and dose dependence. The penetrating effect of charged particles and oxygenation effect of the reactive oxygen species might play a dominant role in plasma-induced bacterial spore inactivation, while the role of electric field is negligible.     

低温等离子体对血压计袖带的消毒效果实验研究

等离子体灭菌技术是消毒学领域继甲醛、环氧乙烷、戊二醛等低温灭菌技术之后又一新的低温灭菌技术,其特点是低温、快速灭菌、毒性残留低,而且对于耐湿热和不耐湿热的医疗器械均适用。由于血压计袖带在医院使用频繁,并且是反复使用,极易被病人体表携带的微生物所污染,故对血压计袖带的消毒已引起医院的重视。而目前医院内对血压计袖带多采用化学消毒液擦拭、紫外线照射或甲醛熏蒸等方法消毒。以上消毒方法有的有较多的毒性残留或有刺激性气味,有的需要较长的消毒时间,而低温等离子体灭菌方法可克服上述缺点。     

Low-temperature Plasma Promotes Fibroblast Proliferation in Wound Healing by ROS-activated NF-κB Signaling Pathway

Low-temperature plasma (LTP) has shown great promise in wound healing, although the underlying mechanism remains poorly understood. In the present study, an argon atmospheric pressure plasma jet was employed to treat L929 murine fibroblasts cultured in vitro and skin wounds in BALB/c mice. The in vitro analysis revealed that treatment of fibroblasts with LTP for 15 s resulted in a significant increase in cell proliferation, secretion of epidermal growth factor (EGF) and transforming growth factor-βi (TGF-βi), production of intracellular reactive oxygen species (ROS), and the percentage of cells in S phase, protein expression of phosphorylated p65 (P-p65) and cyclinD1, but a noted decrease in the protein expression of inhibitor kappa B (IκB). The in vitro experiments demonstrated that 30-s LTP treatment enhanced the number of fibroblasts and the ability of collagen synthesis, while 50-s treatment led to the opposite outcomes. These results suggested that LTP treatment promotes the fibroblast proliferation in wound healing by inducing the generation of ROS, upregulating the expression of P-p65, downregulating the expression of IκB, and activating the NF-κB signaling pathway and consequently altering cell cycle progression (increased DNA synthesis in S phage).     

UF-50全自动尿沉渣分析仪假阳性结果的分析

为了适应尿沉渣分析标准化的需要，UF-50全自动尿沉渣分析仪在临床检验中的应用越来越广泛。我们发现UF-50提供的检测项目有些结果与实际工作存在着不同程度的假阳性。通过对100例尿液标本UF-50假阳性结果和手工离心镜检的分析。得出了各项假阳性结果的影响因素，现报道如下：