高压氧治疗Bell麻痹的系统评价

目的 了解是否有足够的证据表明高压氧治疗Bell麻痹有效.方法 电子检索PubMed、Ovid、TheCochrane central Register of Controlled Trails、EMbase、Science Citation Index Expanded、High Wire Press、中国生物医学文献数据库(cBMDisc)、中国知网(CNKI)、维普数据库(VIP),文献检索起止时间均为从建库到2010年2月,同时检索纳入文献的参考文献,纳入高压氧治疗Bell麻痹的的随机对照试验,进行质量评价及资料提取.统计学分析采用RevMan5.0.22.0软件.结果 共检索到随机对照试验10个,病例823例.所有研究认为高压氧可以提高面神经麻痹的治愈率和有效率.结论 现有证据提示高压氧治疗面神经麻痹很有前途,但是严格设计的RCT证据数量太少,质量有待改进,结果说服力不强,还需要更多高质量的RCT证实其疗效,以便在国内外推广.     

脂肪干细胞侧脑室定向移植改善脑出血大鼠的神经功能

Objective To study improvement of neural function by stereotaxic transplantation of adipose-derived stem cells (ADSC) into lateral cerebral ventricle after intracerebral hemorrhage in rats and its mechanism. Methods ADSC were cultured and proliferated in vitro, which had been marked with Brdu for 48 h before transplantation. The rat caudate nucleus hemorrhage (ICH) models were divided into 2 groups. ADSC were stereotaxically transplanted into the right lateral ventricles in ADSC group, and equal volume of saline was transplanted into control group. The score of neurological behavior were evaluated at modeling and 1, 3, 7, 14, 28 days after transplantation respectively.Double-staining immunofluorescence technique was used to detect Brdu-positive cells and the differentiation of neurons and astrocytes. In accordance with the instructions of TUNEL kit, cell apoptosis, and the expression of VEGF and angiogenesis were assayed. Results In vitro ADSC expressed undergo osteogenic and adipogenic differentiation. Compared with the control group, ADSC group had better motor function at 3, 7, and 14 days (P＜0. 05). Double-staining immunofluorescence showed mostly grafted Brdu-reactive ADSC had migrated to the hematoma zone, and some survivedand expressed Neun of Gfap. TUNEL analysis revealed that, 3 days after transplantation, the number of apoptotic cells in ADSC group was significantly less than in the control group (P＜0. 05). Three days after transplantation, VEGF expression levels in ADSC group were significantly higher than in the control group (P＜0. 05). Conclusion ADSC stereotaxially transplanted into the lateral ventricle can survive and differentiate into neuron-like cells. ADSC transplantation may reduce apoptosis and secret VEGF to promote the angiogenesis, and improve neural functional in intracerebral hemorrhage rats.     

利用人工智能系统预测脑梗死静脉溶栓后的出血转化

目的 利用人工智能系统建立一个有效的预测模型,对脑梗死静脉溶栓后的出血转化进行早期预测.方法 回顾性分析2016年6月至2019年11月南华大学附属长沙中心医院前瞻性注册登记的静脉溶栓患者的资料.收集患者的人口学、临床、理化及影像学指标共53项,利用单因素判别分析建立单因素模型,利用逻辑回归(LR)、朴素贝叶斯(NB)...     

嗅鞘细胞微环境和水凝胶生物支架对脂肪干细胞成嗅鞘样细胞分化的影响

目的 比较研究脂肪干细胞和嗅鞘细胞以两种不同方式共培养后脂肪干细胞P75的阳性表达.方法 以Transwell小室为共培养载体.无支架共培养组以脂肪干细胞接种于上室,嗅鞘细胞接种于下室,支架共培养组以脂肪干细胞联合水凝胶生物支架(BDTM PuraMatrixTM Peptide Hydrogel)接种于上室,嗅鞘细胞接种于下室.检测两组细胞共培养12 d后其脂肪干细胞P75的表达.结果 共培养12 d后无支架共培养组和支架共培养组都有部分细胞表达P75,且支架共培养组表达率更高.结论 脂肪干细胞在嗅鞘细胞生长微环境中能向嗅鞘样细胞分化.     

2型糖尿病患者隐性高血压与颈动脉内中膜厚度关系分析

糖尿病和高血压是心血管事件的危险因素。2型糖尿病患者降压治疗的重要性已经得到证实[1]。临床上高血压通过诊室测量血压获得。然而,通过动态血压监测发现存在很多隐性高血压[2]。隐性高血压是指诊室血压低于140/90mmHg,平均白天动态血压≥135/85mmHg[3]。     

实验性脑出血后大鼠行为学和血肿周围组织病理学的研究

目的观察大鼠脑出血后不同时间点神经行为学和血肿周围脑组织病理学的特点。方法将成年SD大鼠随机分为假手术组和脑出血组;脑出血组大鼠通过立体定向术向脑内注入VII型胶原酶制成脑尾状核出血模型,并按不同时间点（1、3、7、14、28d）分为5个亚组。采用神经功能评分和HE染色分别观察脑出血大鼠神经行为学和脑组织形态学的改变。结果与假手术组相比,脑出血组大鼠的神经行为学评分3d时最明显[3d与1、7d无明显差异（P〉0.05）;与14d和28d有显著差异（P〈0.05）]。脑出血后1d在尾壳核区域可见血肿形成,呈椭圆形或不规则形;3d时脑水肿明显;7d时血肿周围脑组织有胶质细胞增生;14d时血肿区逐渐形成不规则囊腔;28d时囊腔仍然存在,出血周边区见胶质细胞进一步增多。结论脑出血后神经行为学的改变、血肿及囊腔的形成与出血时间有关。     

肺癌患者CDH13基因启动子甲基化状态研究

目的应用甲基化特异性PCR（MSP）方法检测非小细胞肺癌（NSCLC）CDH13基因启动子甲基化情况。方法留取44例非小细胞肺癌患者手术标本及12例非肺癌患者正常肺组织,MSP分析CDH13基因启动子甲基化情况。结果 44例非小细胞肺癌中CDH13基因启动子甲基化阳性率为54.5%（24/44）,12例正常肺组织中未检测到此基因的甲基化（0/12）。CDH13基因甲基化与患者性别及吸烟与否无关;肿瘤分期越晚甲基化的发生率越高。结论原发性非小细胞肺癌中存在着较高比例的CDH13启动子甲基化,提示CDH13在非小细胞肺癌的发生中起着重要作用。     

Neurogenesis of adipose-derived stem cells in hydrogel

Adipose tissue is a readily available source of adult stem cells with multipotent properties suitable for tissue engineering and regenerative medical applications. Peptide hydrogel is a novel biomaterial which provides three-dimensional microenvironments for a variety of cells for tissue grafting. In this study, adipose-derived stem cells (ADSCs) were isolated from rats, seeded into the peptide hydrogel polymer scaffolds and cultured in Neurobasal (NB) media supplemented with B27, basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). Ten days after the culture, some cells were expanded into clonal populations in which the expression of both Nestin and Brdu was detected but only Brdu expression was detected in the cells that were not expanded into clonal populations. Our results suggested that ADSCs in peptide hydrogel polymer scaffolds can be induced to differentiate into cells capable of expressing the neuron-associated markers, self-renewal and self-propagation.     

微创血肿清除术治疗脑出血患者近期生存率及颅外并发症研究

性低钠血症13例,脑心综合征11例.对两组患者1月内生存率及并发症进行统计学分析显示:微创术组生存率优于保守组(P＜0.05);两组患者肺部感染,泌尿系感染,消化道出血,顽固性低钠血症,脑心综合征发生率差异有统计学意义(P＜0.05).结论 微创术治疗基底节脑出血手术简单,创伤小,术后并发症少、生存率较高.