THE EFFECT AND MECHANISM OF PRETREATMENT ON ORGAN TRANSPLANTATION

Pretreatment is one of the methods to prolong organ allograft survival. In this study, Concanavalin A (Con A) was used to pretreat grafts and massive doses of cyclophosphamide and methylprednisolone were used to pretreat donors. The duration of sur. vival after graft pretreatment was significantly longer than that of the grafts without treatment. Light and electron microscopic radioautography, fluorescein labeling method and immunoenzyme technique were used to locate Con A, which was :shown to coat the graft cell membrane. ' Comple- ment dependent cytotoxicity test and second ahti- body labeled with fluorescein technique were used to study the mechanism of Con A which was em- ployed in pretreatment for organ transplantation. It seems reasonable to speculate that Con A could mask the immunogenicity of graft cell antigens by coating the cell membranes and interfering with the perception of the graft antigens by the immune lymphocytes or by the antibodies.     

实时荧光定量PCR检测宫腔脱落细胞中P27~(kip1)及cylin E基因的表达及意义

目的探讨宫腔脱落细胞中P27kip1、cyclin E基因的表达在子宫内膜癌早期诊断中的意义。方法收集健康志愿者及子宫内膜癌患者宫腔脱落细胞样本共80例;提取总RNA,采用实时荧光定量PCR法检测目的基因的表达,比较健康志愿者及子宫内膜癌患者宫腔脱落细胞中P27kip1、cyclin E基因表达量的差异。结果荧光定量PCR结果显示,P27kip1基因在健康志愿者宫腔脱落细胞中的表达显著高于子宫内膜癌患者,差异有统计学意义(P0.05),并随恶性程度升高,P27kip1的表达水平降低,两者比较差异有统计学意义(P0.05);cyclin E基因的表达随恶性程度的增高而增高,两者比较差异有统计学意义(P0.05)。结论联合检测P27kip1和cyclin E基因可作为子宫内膜癌早期诊断的依据。