Tourette综合征患儿血清催乳素水平及神经心理功能的对照研究

目的通过检测Tourette 综合征(TS)患儿血清催乳素(PRL)水平及神经心理功能的变化,探讨TS的发病机制,并为诊断和治疗提供参考.方法采用放射免疫分析法(RIA)测定TS患儿及正常对照组儿童血清PRL水平.采用中国修订韦氏儿童智力量表(WISC-CR)、中国学龄前儿童气质量表(CPTS)及中国学龄儿童气质量表(CSTS)测查TS患儿神经心理特征.结果未用药TS患儿血清PRL水平较对照组明显降低(P<0.001),经神经阻滞剂治疗后PRL水平较未用药组明显升高(P<0.001).TS患儿总智商(FIQ)、言语智商(VIQ)和操作智商(PIQ)与对照组比较差异无显著性(P>0.05),但背数、译码和积木图案分测验分明显低于对照组(P<0.05).TS患儿气质类型与常模组比较有高度显著性差异(P<0.001).结论 TS患儿存在中枢多巴胺(DA)系统过度活跃现象,外周血PRL水平可作为衡量中枢DA系统功能的良好指标,对指导诊断及治疗可能有一定参考意义.TS患儿存有认知功能的某些改变,这种改变可能是TS的一种伴随现象.TS患儿的气质特征有其独特性,提示TS具有遗传学基础.     

手法复位治疗颈性眩晕236例小结

自2000～2004年,作者采用仰卧牵拉侧摆复位法治疗颈性眩晕236例,疗效满意.现总结报告如下. 1临床资料 1.1一般资料本组236例中,门诊病人138例,住院病人98例.男99例,女137例.年龄30岁以下39例,～40岁57例,～50岁79例,50岁以上51例.病程1个月之内41例,～6个月57例,～2年86例,2年以上52例.有外伤史23例.     

肿瘤坏死因子-α与卵巢癌

肿瘤坏死因子-α(TNF-α)通过多种机制参与卵巢癌的生物学变化，TNF-α及其受体分泌量的变化可能与卵巢癌进展有关。近年来采用其治疗卵巢癌，主要集中在利用TNF-α与多种化疗药物产生协同的细胞毒作用以及促使内源性TNF-α的产生来杀伤癌细胞。TNF-α重组蛋白疗法、基因疗法和TNF-α单克隆抗体疗法为卵巢癌的治疗带来希望。     

流感DNA疫苗免疫BALB/c小鼠克服母源性抗体干扰的研究

目的 为了克服母源性抗体对子代的免疫抑制作用,寻找避免母源性抗体干扰的流感疫苗免疫策略.方法 以小鼠为动物模型,接种流感灭活疫苗或DNA疫苗,并用致死量流感病毒感染.感染后检测小鼠的存活率、肺部病毒滴度、体内抗体滴度等指标,对疫苗的保护效果进行评价.结果 母代与子代免疫相同的疫苗,不论是灭活疫苗还是DNA疫苗,子代体内的母源性抗体都抑制了子代免疫后的自动免疫应答,表现为子鼠接种疫苗后不能抵御致死量流感病毒感染;母代免疫流感灭活疫苗,子代免疫神经氨酸酶DNA疫苗,子鼠能够克服母源性抗体干扰,抵御致死量流感病毒感染;母代和子代免疫不同的DNA疫苗,即母代免疫血凝素或神经氨酸酶DNA疫苗,子代免疫神经氨酸酶或血凝素DNA疫苗,也能达到克服母源性抗体干扰的目的 .结论 流感DNA疫苗免疫BALB/c小鼠能克服母源性抗体的干扰,这为临床新生儿抗母源性抗体干扰的研究提供了实验参考.     

转染CD40配体的卵巢癌细胞株OVHM抗卵巢癌肝转移机制的研究

Objective To examine the in vivo anti-metastatic effect of enhanced expression of CD40L cDNA in murinc ovarian cancer OVUM cells (CD4OL-OVHM) injected into the spleen on liver metastasis in mice. Methods OVUM cells were inoculated into the spleen of 6 ～ 8 week-old female B6C3F1 (C57BL/6N × C3H/He) mice. The established liver metastasis was identified by histopathology (HE staining). OVUM cells, DNA-pMKITneo-OVHM cells or CD40L-OVHM cells were inoculated into the spleen of female B6C3F1 mice and the expressions of CD11c in splenic cells were detected by flow cytometry. The specific cytotoxicity of splenic cells was detected by MTT assay, and the serum cytokines of IFN-γ, TNF-α, IL-12, IL4 and IL-10 of the mice were measured by enzyme linked immunoabsorbent assay. The liver metastases and the survival time of the mice were also recorded. Results The mouse models with liver metastasis by injecting tumor cells into the spleen of mice were established. The expression of CD11c and the specific killing rote in CD40L-OVHM cells group was significantly higher than that in the OVHM cells group and DNA-pMKITneo-OVHM cells group. The expressions of IFN-γ, TNF-α and IL-12 in the CD40L-OVHM cells group were much more increased than OVHM cells group and DNA-pMKITneo-OVHM cells group, but the expressions of IL-4 and IL-10 in the CD40L-OVHM cells group were decreased significantly (P < 0.05). The average weights of livers and spleens of mice in CD40L-OVHM cells group were significantly lower than those of DNA-pMKITneo-OVHM cells group and OVHM cells group. The survival time of mice in CD40L-OVHM cells group was also significantly longer than that in the OVHM cells group and DNA-pMKITneo-OVHM cells group (P < 0.05). Conclusion The data directly demonstrate that the expression of CD40L in ovarian cancer cells (CD40L-OVHM) can enhance the proliferation and differentiation of dendritic cells in the spleen and induce specific cytotoxic effect of T cells in the spleen, and may regulate the immune function of peripheral blood cells and the immune balance between Thl cells and Th2 cells, which maybe the possible mechanism induced by CD40L in mice inhibiting the development of liver metastasis.     

小儿急性阑尾炎误诊分析

目的通过对小儿急性阑尾炎术前误诊原因分析,以提高小儿急性阑尾炎术前诊断的准确率。方法对376例中经手术治疗及病理证实为阑尾炎而术前诊断为其他疾病的患儿进行分析,以明确小儿急性阑尾炎术前误诊原因。结果 106例患儿超声确诊99例,X线造影确诊101例。结论病史不明确,症状不典型,检查不配合,是造成小儿急性阑尾炎误诊主要原因。     

木糖醇口香糖对妊娠期牙龈炎和牙菌斑作用的研究

目的评价木糖醇口香糖对妊娠期牙龈炎和牙菌斑的影响,为孕期使用口香糖提供科学依据。方法 选择60例患有妊娠期牙龈炎的患者,基线检查菌斑指数和牙龈指数后随机分为3组,即木糖醇口组、洗必泰组、对照组,均20例。木糖醇组:口腔卫生宣教+康齿宁乳膏刷牙+木糖醇;洗必泰组:口腔卫生宣教+康齿宁乳膏刷牙+洗必泰含漱;对照组:口腔卫生宣教+康齿宁乳膏刷牙。6、12周分别复查菌斑指数和牙龈指数。结果 3组试验前菌斑指数和牙龈指数差异均无统计学意义(P(0.05);6、12周3组菌斑指数、牙龈指数均呈下降趋势,木糖醇组和洗必泰组下降明显(P(0.05),对照组下降不明显(P(0.05);木糖醇组与对照组比较差异有统计学意义(P(0.05),木糖醇组与洗必泰组比较差异无统计学意义(P(0.05)。结论在同等的口腔环境下,木糖醇口香糖和洗必泰对口腔卫生均有显著改善。     

针药结合治疗内分泌失调性不孕症23例

女子婚后,配偶生殖功能正常,夫妇同居2年以上未避孕而不怀孕者,称为原发性不孕;曾孕育过,并未采取避孕措施,又间隔2年以上未再孕,称为继发性不孕;统称不孕症。笔者近3年应用针药结合治疗不孕症23例,疗效满意,报道如下。1临床资料1·1一般资料:23例患者均为门诊病人,年龄26~32岁     

CCK-8上调小鼠腹腔巨噬细胞B7.1和B7.2表达并增强其协同刺激活性

目的： 探讨八肽胆囊收缩素（CCK-8）对静息巨噬细胞B7.1和B7.2表达及其协同刺激功能的影响。方法：用CCK-8（10-12-10-6 mol/L）孵育小鼠腹腔巨噬细胞一定时间，采用流式细胞术分析细胞表面B7.1和B7.2含量的变化。用免疫磁珠从小鼠脾细胞分离CD4+T细胞，按4∶〖KG-*2〗1数量比与腹腔巨噬细胞（预先用CCK-8和/或抗B7.1抗体、抗B7.2抗体、CCK1R拮抗剂CR1409、CCK2R拮抗剂CR2945孵育24 h）共同体外培养，同时加入ConA 5 mg/L，采用［3H］掺入法测定CD4+T细胞增殖反映巨噬细胞的协同刺激活性。结果：CCK-8可上调静息巨噬细胞B7.1及B7.2的表达，并增强巨噬细胞的协同刺激活性。CCK-8的作用呈剂量依赖性，最大效应剂量是在10-9-10-7 mol/L之间。抗B7.2抗体可减轻CCK-8增强巨噬细胞协同刺激活性的作用，CR1409及CR2945均能逆转CCK-8的上述作用，且CR1409的作用较CR2945更明显。结论：CCK-8通过上调巨噬细胞B7.2表达而增强其协同刺激活性，该作用由CCK1R及CCK2R介导，其中CCK1R起主要介导作用。