对氧磷酶1与呼吸系统疾病关系的研究进展

对氧磷酶1是一种钙依赖糖蛋白,主要生理作用是防止低密度脂蛋白和高密度脂蛋白氧化修饰,抗动脉粥样硬化,此外还具有对抗细菌内毒素毒性以及对有机磷毒性的保护等作用。本文将对对氧磷酶1的结构、功能、生物特性及其与呼吸系统疾病之间的关系作一综述。     

慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

Objective To analyze the activity of paraoxonase (PON1) and explore the relationship of PON1 and oxidative stress with systemic inflammation response in the acute exacerbation phase and stationary phase in patients with chronic obstructive pulmonary disease (COPD). Methods Serum PON1 activity was measured by phenylacetate in 38 patients with COPD and 30 healthy people. The activity of glutathione peroxidase (GSH-Px) was detected by improved Hafeman method. Total antioxidant capacity (TAC) was measured by eolorimetry and malondialdehyde (MDA) level was measured by thiobarbituric acid colouration method. The interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were detected by radioimmunoassay. The level of C-reactive protein (CRP) was measured by immune turbidimetry. Results In the acute exacerbation phase, the activity of serum PON1 was significantly lower in COPD group than in control group [(98.03±42.40)×103U/L vs. (136.00±60. 50)×103U/L, t=4.962, P<0.01], and it was negatively related to the IL-8 level (r= - 0. 589, P<0.01) and positively related to FEV1% (r= 0. 434, P<0. 05). The activity of GSH-Px was negatively related to the IL-6 level (r=-0. 362, P< 0. 05). In the stationary phase of COPD group, the activity of serum PON1 had no statistical difference compared with control group[(131.50±53.65))×103U/L vs. (136. 00±60.50)×103U/ L, t=2. 457, P>0. 053, and it was negatively related to the IL-8 level (r=-0. 563, P<0.05). Conclusions Serum PON1 activity is significantly decreased in acute exacerbation phase of COPD group compared with control group and it is positively related to FEV1%. The oxidative stress is closely related to systemic inflammation response in patients with acute exacerbation of COPD.     

高频超声用于腹股沟疝患者诊断中的临床效果

目的在腹股沟疝患者诊断之中应用高频超声取得的临床效果,探究高频超声在腹股沟疝诊断中的应用价值。方法将2016年02月25日至2018年02月28日在我院进行检查的23例腹股沟疝患者纳入该次研究,患者均进行了高频超声检查,回顾性分析临床资料并研究患者的检查情况。将检查结果和手术结果进行对比,了解高频超声的临床应用价值。结果 23例患者均进行了高频超声检测,其中17例患者被诊断为腹股沟斜疝,股疝4例,直疝2例患者;手术结果中斜疝共16例,股疝5例,直疝2例,超声诊断符合率为95.65%。疝内容物复杂且多样,声像图表现结果各有不同。若包块呈实性,回声高低不均时,疝内容物多为大网膜。但是形态和疝囊较相似,彩色血流基本无血流信号。若显示墙壁结构,肠腔内见肠内内容物杂乱回声光电,结果显示肠管形态、位置出现改变,见肠蠕动,彩色血流能够探及肠壁上星点状和短线状血流信号,则疝内容物为肠管。超声灵敏度为95.6%,特异性为43.9%,阳性预测值为90.8%,阴性预测值为61.3%。结论腹股沟疝患者采用高频超声进行诊断,通过清晰的图像能更全面且精准的了解疝内容物,能够为临床提供清晰更加有效的科学依据,且安全无创,可以进行重复诊断,观察疝囊结构、疝内容物大小、形态、位置,而且可以将其和其他腹股沟区肿块展开鉴别,临床治疗提供清晰的图像,值得应用在临床应用。     

慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

Objective To analyze the activity of paraoxonase (PON1) and explore the relationship of PON1 and oxidative stress with systemic inflammation response in the acute exacerbation phase and stationary phase in patients with chronic obstructive pulmonary disease (COPD). Methods Serum PON1 activity was measured by phenylacetate in 38 patients with COPD and 30 healthy people. The activity of glutathione peroxidase (GSH-Px) was detected by improved Hafeman method. Total antioxidant capacity (TAC) was measured by eolorimetry and malondialdehyde (MDA) level was measured by thiobarbituric acid colouration method. The interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were detected by radioimmunoassay. The level of C-reactive protein (CRP) was measured by immune turbidimetry. Results In the acute exacerbation phase, the activity of serum PON1 was significantly lower in COPD group than in control group [(98.03±42.40)×103U/L vs. (136.00±60. 50)×103U/L, t=4.962, P<0.01], and it was negatively related to the IL-8 level (r= - 0. 589, P<0.01) and positively related to FEV1% (r= 0. 434, P<0. 05). The activity of GSH-Px was negatively related to the IL-6 level (r=-0. 362, P< 0. 05). In the stationary phase of COPD group, the activity of serum PON1 had no statistical difference compared with control group[(131.50±53.65))×103U/L vs. (136. 00±60.50)×103U/ L, t=2. 457, P>0. 053, and it was negatively related to the IL-8 level (r=-0. 563, P<0.05). Conclusions Serum PON1 activity is significantly decreased in acute exacerbation phase of COPD group compared with control group and it is positively related to FEV1%. The oxidative stress is closely related to systemic inflammation response in patients with acute exacerbation of COPD.     

肺结核患者血清中肿瘤坏死因子(TNF-α)、瘦素、脂联素水平研究

目的:研究肺结核患者血清中肿瘤坏死因子(TNF-α)、瘦素、脂联素水平,为进一步治疗结核病提供合理的理论依据。方法:采用随机数字表法,选择40例肺结核病患者为试验组,并以同期健康体检者50例作对照组进行观察。比较两组在空腹时血清的肿瘤坏死因子(TNF-α)、瘦素、脂联素水平。结果:试验组肺结核患者血清中的肿瘤坏死因子(TNF-含量明显高于对照组(P<0.05),脂联素的含量却比对照组的低(P<0.05),而瘦素含量差异无统计学意义(P>0.05)。结论:肺结核患者血清中肿瘤坏死因子(TNF-α)、脂联素均参与了细胞免疫过程,而瘦素与反应没有关系,影响瘦素含量变化的重要因素为体重。     

平均床值法摆位技术在颈椎骨转移瘤调强放疗中的应用研究

目的 运用千伏级的锥形束CT（cone beam CT，CBCT）图像引导扫描技术，研究平均床值法摆位技术在颈椎骨转移瘤调强放疗中应用的可行性。方法 每位患者总共扫描30次CBCT，记录每次左右方向x、前后方向y、上下方向z三个方向的数值，取前5次CBCT扫描平均值x₅=（x₁+x₂+…x₅）/5，y₅=（y₁+y₂+…y₅）/5，z₅=（z₁+z₂+…z₅）/5，作为日后摆位床值，与物理师的计划床值x₀、y₀、z₀进行分析，前5次左右方向摆位平均误差Lat₅=x₅-x₀，前后方向摆位平均误差Lng₅=y₅-y₀，上下方向摆位平均误差Vrt₅=z₅-z₀，用前5次摆位误差的平均值表示系统误差，用所有分次的摆位误差的标准差表示随机误差，进行独立t检验。结果 平均床值法摆位技术的系统误差和随机误差无显著性差异（P>0.05），在左右、前后、上下三个维度的误差都<0.3 cm，在允许范围内。结论 平均床值法摆位技术运用在颈椎骨转移瘤的调强放疗是可行的。     

非小细胞肺癌患者血清中组织蛋白酶S降解的巢蛋白-1检测的临床意义

摘 要：［目的］ 探讨非小细胞肺癌（NSCLC）患者血清中组织蛋白酶S降解的巢蛋白-1定量检测的临床意义。［方法］ 选择2010年1月至2012年12月来接受手术切除治疗的NSCLC患者48例,另选择同期年龄、性别48人正常人群作为对照。应用酶联免疫吸附法检测NSCLC患者及对照组中血清巢蛋白-1表达水平。应用单因素、多因素非条件Cox回归分析分析影响NSCLC患者预后的危险因素分析。［结果］ 48例NSCLC患者中血清巢蛋白-1表达水平为（105.62±25.67）ng/ml,显著性高于对照组的（34.59±8.26）ng/ml（P＜0.05）。NSCLC患者中血清巢蛋白-1表达水平与临床病理特征的关系结果显示,血清巢蛋白-1高表达与性别、年龄、吸烟史、嗜酒史、组织类型无相关性（P＞0.05）,与淋巴结转移、分化程度、TNM分期显著性相关（P＜0.05）。Kaplan-Meier生存分析结果显示,血清巢蛋白-1≤70ng/ml的NSCLC患者生存率显著性高于血清巢蛋白-1＞70ng/ml患者（P＜0.05）。多因素Cox回归分析结果显示,淋巴结转移、TNM分期、血清巢蛋白-1水平＞70ng/ml不利于NSCLC患者预后（P＜0.05）。［结论］ NSCLC患者中血清巢蛋白-1水平升高,血清巢蛋白-1水平可作为预测NSCLC患者预后的血清学标志物。     

慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

Objective To analyze the activity of paraoxonase (PON1) and explore the relationship of PON1 and oxidative stress with systemic inflammation response in the acute exacerbation phase and stationary phase in patients with chronic obstructive pulmonary disease (COPD). Methods Serum PON1 activity was measured by phenylacetate in 38 patients with COPD and 30 healthy people. The activity of glutathione peroxidase (GSH-Px) was detected by improved Hafeman method. Total antioxidant capacity (TAC) was measured by eolorimetry and malondialdehyde (MDA) level was measured by thiobarbituric acid colouration method. The interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were detected by radioimmunoassay. The level of C-reactive protein (CRP) was measured by immune turbidimetry. Results In the acute exacerbation phase, the activity of serum PON1 was significantly lower in COPD group than in control group [(98.03±42.40)×103U/L vs. (136.00±60. 50)×103U/L, t=4.962, P<0.01], and it was negatively related to the IL-8 level (r= - 0. 589, P<0.01) and positively related to FEV1% (r= 0. 434, P<0. 05). The activity of GSH-Px was negatively related to the IL-6 level (r=-0. 362, P< 0. 05). In the stationary phase of COPD group, the activity of serum PON1 had no statistical difference compared with control group[(131.50±53.65))×103U/L vs. (136. 00±60.50)×103U/ L, t=2. 457, P>0. 053, and it was negatively related to the IL-8 level (r=-0. 563, P<0.05). Conclusions Serum PON1 activity is significantly decreased in acute exacerbation phase of COPD group compared with control group and it is positively related to FEV1%. The oxidative stress is closely related to systemic inflammation response in patients with acute exacerbation of COPD.     

慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

Objective To analyze the activity of paraoxonase (PON1) and explore the relationship of PON1 and oxidative stress with systemic inflammation response in the acute exacerbation phase and stationary phase in patients with chronic obstructive pulmonary disease (COPD). Methods Serum PON1 activity was measured by phenylacetate in 38 patients with COPD and 30 healthy people. The activity of glutathione peroxidase (GSH-Px) was detected by improved Hafeman method. Total antioxidant capacity (TAC) was measured by eolorimetry and malondialdehyde (MDA) level was measured by thiobarbituric acid colouration method. The interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were detected by radioimmunoassay. The level of C-reactive protein (CRP) was measured by immune turbidimetry. Results In the acute exacerbation phase, the activity of serum PON1 was significantly lower in COPD group than in control group [(98.03±42.40)×103U/L vs. (136.00±60. 50)×103U/L, t=4.962, P<0.01], and it was negatively related to the IL-8 level (r= - 0. 589, P<0.01) and positively related to FEV1% (r= 0. 434, P<0. 05). The activity of GSH-Px was negatively related to the IL-6 level (r=-0. 362, P< 0. 05). In the stationary phase of COPD group, the activity of serum PON1 had no statistical difference compared with control group[(131.50±53.65))×103U/L vs. (136. 00±60.50)×103U/ L, t=2. 457, P>0. 053, and it was negatively related to the IL-8 level (r=-0. 563, P<0.05). Conclusions Serum PON1 activity is significantly decreased in acute exacerbation phase of COPD group compared with control group and it is positively related to FEV1%. The oxidative stress is closely related to systemic inflammation response in patients with acute exacerbation of COPD.     

慢性阻塞性肺疾病患者对氧磷酶和氧化应激与全身炎性反应的研究

Objective To analyze the activity of paraoxonase (PON1) and explore the relationship of PON1 and oxidative stress with systemic inflammation response in the acute exacerbation phase and stationary phase in patients with chronic obstructive pulmonary disease (COPD). Methods Serum PON1 activity was measured by phenylacetate in 38 patients with COPD and 30 healthy people. The activity of glutathione peroxidase (GSH-Px) was detected by improved Hafeman method. Total antioxidant capacity (TAC) was measured by eolorimetry and malondialdehyde (MDA) level was measured by thiobarbituric acid colouration method. The interleukin-6 (IL-6) and interleukin-8 (IL-8) levels were detected by radioimmunoassay. The level of C-reactive protein (CRP) was measured by immune turbidimetry. Results In the acute exacerbation phase, the activity of serum PON1 was significantly lower in COPD group than in control group [(98.03±42.40)×103U/L vs. (136.00±60. 50)×103U/L, t=4.962, P<0.01], and it was negatively related to the IL-8 level (r= - 0. 589, P<0.01) and positively related to FEV1% (r= 0. 434, P<0. 05). The activity of GSH-Px was negatively related to the IL-6 level (r=-0. 362, P< 0. 05). In the stationary phase of COPD group, the activity of serum PON1 had no statistical difference compared with control group[(131.50±53.65))×103U/L vs. (136. 00±60.50)×103U/ L, t=2. 457, P>0. 053, and it was negatively related to the IL-8 level (r=-0. 563, P<0.05). Conclusions Serum PON1 activity is significantly decreased in acute exacerbation phase of COPD group compared with control group and it is positively related to FEV1%. The oxidative stress is closely related to systemic inflammation response in patients with acute exacerbation of COPD.