介入腔内溶栓综合治疗下肢深静脉血栓形成35例分析

目的探讨下肢深静脉血栓形成（DVT）行介入腔内溶栓、抗凝综合治疗的疗效。方法35例DVT在数字减影血管造影（DSA）下放置下腔静脉滤器,腔内导管直接插入静脉血栓处,冲击、持续注入尿激酶溶栓,术后患肢末端静脉输注尿激酶溶栓,联合静脉抗凝综合治疗。结果35例患者,25例血栓完全消失,7例血栓部分残留,3例无改变;治疗结束时肢体健患侧周径差明显缩小;总治愈率达91．4％;与对照组比较有显著性差异（P〈0．01）。治疗过程中未出现严重并发症。结论采用介入腔内溶栓综合治疗DVT疗效好而且安全。     

线粒体DNA在脓毒血症患者中的发病机制及对血栓形成的影响

目的探讨线粒体DNA在脓毒血症患者中的发病机制及对血栓形成的影响。方法取2015年8月至2017年3月ICU收治的脓毒症患者123例,设为观察组。根据患者疾病严重程度分为脓毒血症组(n=70)和严重脓毒血症组(n=53)。取同期入院健康体检30例,设为对照组。采用实时荧光定量PCR技术检测各组血浆mt-DNA水平,观察线粒体DNA在脓毒血症患者中的发病机制及血栓形成发生率的影响。结果观察组(脓毒血症组、严重脓毒血症组)线粒体DNA水平,均高于对照组(P0.05);严重脓毒血症组线粒体DNA水平,高于脓毒血症组(P0.05);严重脓毒血症组血栓发生率为13.21%高于脓毒血症组的4.28%(P0.05)。结论脓毒血症患者线粒体DNA水平升高,且与血栓发生率呈正相关。     

巨噬细胞中脂多糖诱导微小RNA-155表达与地塞米松的抑制

BACKGROUND: It is unclear about dexamethasone effect on the regulation of microRNA-155 expression in macrophages. OBJCTIVE: To explore whether dexamethasone can regulate the expression of microRNA-155 in macrophages. METHODS: (1) Lipopolysaccharide stimulation of mouse macrophages: mouse macrophage cell lines, Raw264.7 cells, were cultured in vitro and stimulated by lipopolysaccharide. Cultured cells were collected at 0, 0.5, 2, 6 hours after culture to detect the dynamical expression of microRNA-155. (2) Dexamethasone intervention for macrophages: Macrophages were divided into four groups: control group treated with phosphate buffer; lipopolysaccharide group stimulated by lipopolysaccharide; combined group given intervention with dexamethasone and lipopolysaccharide; dexamethasone group cultured with dexamethasone. At 6 hours after culture, cell supernatant was collected to detect the expression of tumor necrosis factor α and interleukin-6 using ELISA method. Real-time fluorescence quantitative PCR was used to detect the expression of microRNA-155 in the Raw264.7 macrophages. RESULTS AND CONCLUSION: Lipopolysaccharide significantly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 after 6 hours of culture (P < 0.05). Combined use of dexamethasone and lipopolysaccharide slightly increased the expression of tumor necrosis factor α, interleukin-6 and microRNA-155 (P < 0.05). Dexamethasone alone had no influence on the expression of tumor necrosis factor α and interleukin-6, but significantly decreased the expression of microRNA-155 (P < 0.05). These findings indicate that dexamethasone can inhibit the expression of microRNA-155 in the macrophages induced by lipopolysaccharide.      

外伤性脾破裂的CT诊断

目的 探讨CT检查对外伤性脾破裂的临床价值。材料与方法 回顾分析了33例脾破裂的CT表现及临床过程。结果 (1)包膜下出血主要表现为包膜下新月征和分层状影；（2）实质出血表现为脾实质内密度不均影；（3）有利于估计腹腔内出血量的估计。结论 CT能对脾破裂作出明确的诊断。     

免疫检查点相关基因预测结肠癌患者预后及其免疫状态

目的 免疫检查点基因是调控结肠癌患者免疫应答的关键机制,并对结肠癌患者的预后、治疗具有潜在的临床应用价值,然而目前尚无基于免疫检查点基因的预后模型来预测结肠癌患者的预后和免疫状态。方法 从肿瘤基因组图谱(The Cancer Genome Atlas,TCGA)和高通量基因表达(Gene Expression Omnibus,GEO)公共数据库获取并整理结肠癌患者的基因表达谱数据及其对应的临床信息,鉴定差异表达的免疫检查点基因。采用最小绝对收缩和选择算子(least absolute shrinkage and selection operator,LASSO)Cox回归分析构建免疫检查点基因相关的TCGA结肠癌患者预后模型。应用GEO结肠癌患者队列(GSE143985)进行验证。KaplanMeier曲线和受试者操作特征(receiver operating characteristic,ROC)曲线用于评估模型预测准确性。Spearman相关性分析观察预后模型与免疫细胞浸润情况。单因素和多因素回归分析免疫检查点风险基因及临床指标,并基于多因素回归结果构建临床列线图。结果 在正常和结...