P2X7受体蛋白表达对胰腺癌的发生及发展的影响

目的探讨胰腺癌组织中P2X7受体蛋白表达及与患者临床病理学特征之间的关系。方法选取本院病理科收集的手术后胰腺癌组织80例为病例组、既往病理科收集的20例正常胰腺组织石蜡块为对照组,收集时间2013年1月-2017年1月,应用免疫组织化学染色法(SP)检测两组标本中的P2X7受体蛋白表达水平,并分期P2X7受体蛋白表达与患者临床分期、肿瘤分化、淋巴结转移等特征的关系。结果病例组胰腺癌组织中的P2X7受体蛋白阳性表达率66.25%显著的高于对照组胰腺组织中的10.00%,差异具有统计学意义(P0.05);UICC分期(Ⅲ期)胰腺癌组织的P2X7受体蛋白阳性表达率、低分化程度的P2X7受体蛋白阳性表达率、发生淋巴结转移的P2X7受体蛋白阳性表达率均分别显著的高于UICC分期(Ⅰ期+Ⅱ期)、高+中分化、未发生淋巴结转移的胰腺癌组织,差异具有统计学意义(P0.05);经Logistic回归分析,UICC分期为Ⅲ期、组织学低分化、P2X7受体蛋白阳性表达是胰腺癌患者12个月随访不良结局的独立危险因素(P0.05)。结论胰腺癌组织中P2X7受体蛋白表达上调,并且与UICC分期、肿瘤分化及发生淋巴结转移密切相关。     

早期乳腺癌保乳手术的临床分析

目的:探讨早期乳腺癌前哨淋巴结活检阴性时,保乳手术不清扫腋窝淋巴结的疗效.方法:2006年8月～2009年8月进行保乳手术的26例前哨淋巴结活检均为阴性的早期乳腺癌患者,收集临床资料,分析其手术效果,并进行美容效果评价.结果:26例手术全部成功,随访1～4年,疗效满意.结论:前哨淋巴结活检阴性的早期乳腺癌保乳手术无需行腋窝淋巴结清扫,可取得良好的治疗和美容效果.     

免夹腹腔镜阑尾切除术96例临床分析

目的探讨急慢性阑尾炎行免夹腹腔镜阑尾切除术（LA）的临床经验与应用价值。方法回顾性分析我院微创外科2011年5月-2013年9月96例行免夹腹腔镜阑尾切除术的临床资料。结果共治疗96例,无中转手术,手术时间平均45min,术后住院时间平均（4±2）d。术后随访1~24个月,并发盆腔小脓肿1例,经抗炎治疗痊愈,其余95例无并发症,效果满意。结论免夹腹腔镜阑尾切除术具有探查清楚、微创、康复快、美容、不易出现粘连性肠梗阻等优点,是值得推荐的手术方法。     

人脂肪干细胞对兔任意型皮瓣成活的影响

Objective To explore the effect of the adipose derived stem cells (ADSCs)on the survival of random pattern skin flap in rabbits. Methods ADSCs were isolated from fresh human fat and expanded in vitro for five passages. Then the characterization of ADSCs were determined by their CD marker profile and their ability to differentiate into osteogenic, adipogenic and chondrogenic lineages. On the back of the rabbits, two symmetric cephalic-based random pattern skin flaps were designed (6 cm × 2cm). The right flaps were used as experimental groups with the contra-side flaps as control group. Human ADSCs were pre-labeled before seeding with fluorescent 3, 30-dioctadecyloxacarbocyanine perchlorate (DiO) dye. The experimental flaps evenly received 5-point injection of 2 × 106Dio-labeled ADSCs resuspended in 0.5 ml of serum-free DMEM, while only 0. 5 ml medium was injected into the control flaps.Seven days later, the survival rate of flaps was evaluated. The flaps underwent frozen section and were observed under the laser scanning confocal microscope to detect the fluorescence imaging. Flaps also underwent HE staining and were observed under light microscope to detect the vascular density. Results Compared with control group, there was a significant increase of flap survival rate in the experimental group(P <0.01). Histological analysis also demonstrated a statistically significant increase in capillary density in the experimental group. Conclusions It suggests that ADSCs have a better immune compatibility and potential for enhancing the blood supply of random pattern skin flaps.     

单侧唇裂鼻畸形Ⅰ期矫正临床分析

目的:探讨婴儿期早期修复唇裂,同期矫正鼻畸形的效果。方法:采用Millard术式,同时经唇裂手术切口入路恢复大翼软骨、鼻肌的正常解剖位置,通过剪断口轮匝肌和鼻前棘的异位附着点纠正鼻小柱的偏斜,将患侧鼻翼软骨提到与健侧同一高度,并行贯穿褥式缝合固定。结果:共治疗26例患儿,随访6~26个月,效果满意。结论:在唇裂修复同期进行鼻畸形的矫正,可获得即刻的手术效果和较为满意的远期疗效,该方法能消除严重的唇裂鼻畸形,并可能降低再次手术及手术难度。对于单侧唇裂鼻畸形患者而言,是值得推荐的手术方法。     

人脂肪干细胞对兔任意型皮瓣成活的影响

Objective To explore the effect of the adipose derived stem cells (ADSCs)on the survival of random pattern skin flap in rabbits. Methods ADSCs were isolated from fresh human fat and expanded in vitro for five passages. Then the characterization of ADSCs were determined by their CD marker profile and their ability to differentiate into osteogenic, adipogenic and chondrogenic lineages. On the back of the rabbits, two symmetric cephalic-based random pattern skin flaps were designed (6 cm × 2cm). The right flaps were used as experimental groups with the contra-side flaps as control group. Human ADSCs were pre-labeled before seeding with fluorescent 3, 30-dioctadecyloxacarbocyanine perchlorate (DiO) dye. The experimental flaps evenly received 5-point injection of 2 × 106Dio-labeled ADSCs resuspended in 0.5 ml of serum-free DMEM, while only 0. 5 ml medium was injected into the control flaps.Seven days later, the survival rate of flaps was evaluated. The flaps underwent frozen section and were observed under the laser scanning confocal microscope to detect the fluorescence imaging. Flaps also underwent HE staining and were observed under light microscope to detect the vascular density. Results Compared with control group, there was a significant increase of flap survival rate in the experimental group(P <0.01). Histological analysis also demonstrated a statistically significant increase in capillary density in the experimental group. Conclusions It suggests that ADSCs have a better immune compatibility and potential for enhancing the blood supply of random pattern skin flaps.     

人脂肪干细胞的分离培养及鉴定

目的:探索从健康成人脂肪组织中分离、培养并鉴定脂肪干细胞.方法:用健康成人的脂肪组织,剔除可见纤维结缔组织后剪碎,胶原酶法分离并体外培养脂肪干细胞,并进行流式细胞术鉴定.结果:可以从健康成人脂肪组织中提取出脂肪干细胞,体外环境扩增传代,流式细胞仪检测细胞表面高度表达CD29,CD44,CD105等,具有间充质干细胞特性.结论:可以从人脂肪组织中分离、培养出脂肪干细胞.     

脂肪来源干细胞的研究及临床应用前景

目的：总结脂肪干细胞的研究进展及在临床上的应用。方法：查阅国内外相关文献,对脂肪干细胞的分化能力及临床应用前景进行汇总分析。结果：脂肪干细胞具有多向分化潜能,在动物模型中进行组织修复、细胞移植等方面有成功报道。结论：作为种子细胞的脂肪干细胞有着广阔的临床应用前景,其研究有待进一步深入。