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Objective To identify,construct and express scFv CD133,verify its biological function.Methods VL and VH were isolated from hybridoma of mAb CD133 by using antibody engineering technology.Its DNA sequencing and CDR were determined.scFv CD133 was then cloned into pET32a,transformed into Origami,induced by IPTG,purified by Ni2+-NTA His resin.Its affinity and specificity were tested by NH4SCN elution and ELISA.Results The size of VL and VH of scFv CD133 was 339 bp and 342 bp,which coded 113 and 114 amino acid separately.Its VL belonged to mouse Igκ chain and VH belonged to mouse IgG heavy chain subtype I.The molecular weight of scFv CD133 was about 27 × 103 which was testified by SDSPAGE and Western blot.Its affinity and specificity were also verified.Conclusion scFv CD133 has been successfully constructed and expressed in Origami,which could supply basis for target therapy of CD+133 cancer stem cell. 相似文献
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目的对比分析贲门失弛缓患者经口内镜下肌切开术(POEM)与经胸食管贲门部黏膜外肌层切开手术(即Heller术)的临床疗效及安全性。方法回顾性研究2012年1月-2014年10月该院78例确诊为贲门失弛缓症患者。其中,接受POEM术患者为42例,接受Heller术患者36例,观察并对比两组患者术后症状缓解情况、食管下段括约肌(LES)静息压力、Eckardt评分、术中和术后并发症发生率、手术时长、住院时间及住院费用等。结果两组患者术后均获得明显的临床缓解,POEM组患者的并发症发生率、Eckardt评分及LES静息压力和Heller术组相比差异无统计学意义(P0.05)。而POEM组患者的手术耗时、住院时长及住院费用均明显低于Heller术组(P0.05)。结论 POEM作为一种近期疗效显著的内镜微创手术方式,与外科手术疗效相当,对患者造成的创伤小,痛苦小,使患者耐受性好,住院时间及住院费用减少,值得推广。 相似文献
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[目的]探讨细胞因子诱导杀伤细胞(CIK细胞)在消化道癌中的疗效.[方法]选取2008年9月~2010年6月干本院进行治疗的88例消化道癌患者为研究对象,将其随机分为对照组(化疗组)44例和观察组44例,后将两组患者的不良反应发生率、SF-36总分及治疗前后的外周血T淋巴细胞亚群、血清CA199、CA242、CEA、A... 相似文献
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IL 6及其受体α链 (sIL 6Rα )、β链 (sIL 6Rβ )和T细胞亚群在机体抗肿瘤免疫中起着重要作用 ,其表达水平反映了淋巴细胞的活化程度 ,可提示机体的抗肿瘤免疫状况。本文以上述指标评价消化道肿瘤的免疫功能水平。1 材料和方法1 1 研究对象 食道癌 11例 ,胃癌 10例 ,原发性肝癌 8例 ,胰腺癌 5例 ,以上均为初诊患者。健康对照组 (NC组 )为健康献血员 30例。1 2 实验方法1 2 1 IL 6、sIL 6Rα及sIL 6Rβ测定 :抽取受检者肝素钠抗凝血 ,收集血浆 , 2 0℃保存待测 ,IL 6及sIL 6Rα试剂系法国Immunot… 相似文献
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目的总结无痛内镜黏膜下剥离术(ESD)治疗早期胃癌的手术经验,以提高ESD的成功率并减少手术并发症的发生。方法将50例经胃镜及病理确诊为早期胃癌并采用ESD行病变切除治疗的患者随机分为局麻组、全麻组,每组25例。局麻组行表面麻醉并鼻导管吸氧,全麻组行表面麻醉联合气管插管全麻。监测患者ECG、HR、SPO2、MAP,术后对ESD操作情况和患者术中疼痛进行评价。结果局麻组术中MAP、HR均高于其基础值,也高于全麻组(P<0.05);全麻组患者ESD操作评分、镇痛效果均优于局麻组(P<0.05)。结论表面麻醉联合气管插管全麻较单纯表面麻醉下进行ESD更安全有效,且并发症更少。 相似文献
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目的 探讨内镜超声(EUS)对胃异位胰腺的诊断价值.方法 回顾性分析23例病理诊断胃异位胰腺的EUS特征,主要指标包括病变起源层次、内部回声特征、边缘、边界、长短轴比例、病变处固有肌层厚度等.结果 23例患者中超声内镜诊断符合率91%(21/23).EUS下异位胰腺可起源于胃壁的第二、第三或第四层(5层结构),其中起源于第三层者最多见(15/23,65%).病变多呈中等不均匀回声,内部有管道样结构或无回声区者占43%(10/23),边界不清者占70%(16/23).异位胰腺平均长短轴比例 1.7;病变起源于第二和(或)第三层的病变,局部固有肌层较周围增厚者占71%(12/17).结论 EUS对胃异位胰腺的诊断正确性较高.典型的胃异位胰腺EUS特征包括:壁内侧向生长,黏膜下层起源,边界不规则、不清,中等不均匀回声,内部有无回声区,固有肌层增厚.Abstract: Objective To describe the characteristic endoscopic ultrasonography (EUS) features of gastric ectopic pancreas.Methods Totally 23 patients were diagnosed pathologically as having gastric ectopic pancreas.The EUS images of the lesions were retrospectively reviewed regarding layer of origin,size,growth pattern,margin,and internal echo pattern.Results The coincidence of EUS and pathologic diagnosis was 91%(21/23).EUS revealed that the lesions originated from the second,third,and/or fourth layers of the gastric wall.Most lesions were heterogenous,mainly isoechoic.The borders of the lesions were indistinct in 70%(16/23) patients. Anechoic cystic or tubular structures within the lesions appeared in 10 of the 23 lesions(43%).The mean longest/shortest diameter ratio of ectopic pancreas was 1.7.Conclusions Characteristic EUS features of gastric ectopic pancreases include:mural lateral growth pattern,third layer (submucosa) origin,indistinct margin,intermediate echogenecity,anechoic areas,and fourth-layer thickening.Careful assessment of the EUS findings may be a useful aid in the diagnosis of gastric ectopic pancreas. 相似文献
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