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排序方式: 共有1406条查询结果,搜索用时 15 毫秒
1.
Lou Zhong-jin罗仲金 Wang Ling王玲 Liu Qing 刘庆Tian Guang-zhao田光兆 Hu Xiao-su胡孝素 Huang Guang-qi黄光琦Qian Fang钱方 Ran Rui-tu冉瑞图West China University of Medical Sciences ChengduHuang De-huai黄德淮Chengdu Fifth People''s Hospital Chengdu 《中华医学杂志(英文版)》1987,100(9):740-744
Comparative studies are made on the relative
percentages of amino acid content and LDH isozyme
by electrophoresis and Slab-PAGE in adult worms
of Ascaris lumbricoides, Ascaris suum and Toxocara
canis. The results show that there are remarkable
differences of Arg and His of 17 amino acids bet.
ween Toxocara canis and human intestinal ascaris,
human biliary ascaris and pig ascaris; there are also
obvious differences of band positions of LDH
isozyme electrophoretic patterns and band position
and amount of slab-PAGE patterns among them,
whereas no marked differences are found in these
three indices in human intestinal ascaris, human
biliary ascaris and pig ascaris. 相似文献
2.
Sequence variation within botulinum neurotoxin serotypes impacts antibody binding and neutralization
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Smith TJ Lou J Geren IN Forsyth CM Tsai R Laporte SL Tepp WH Bradshaw M Johnson EA Smith LA Marks JD 《Infection and immunity》2005,73(9):5450-5457
The botulinum neurotoxins (BoNTs) are category A biothreat agents which have been the focus of intensive efforts to develop vaccines and antibody-based prophylaxis and treatment. Such approaches must take into account the extensive BoNT sequence variability; the seven BoNT serotypes differ by up to 70% at the amino acid level. Here, we have analyzed 49 complete published sequences of BoNTs and show that all toxins also exhibit variability within serotypes ranging between 2.6 and 31.6%. To determine the impact of such sequence differences on immune recognition, we studied the binding and neutralization capacity of six BoNT serotype A (BoNT/A) monoclonal antibodies (MAbs) to BoNT/A1 and BoNT/A2, which differ by 10% at the amino acid level. While all six MAbs bound BoNT/A1 with high affinity, three of the six MAbs showed a marked reduction in binding affinity of 500- to more than 1,000-fold to BoNT/A2 toxin. Binding results predicted in vivo toxin neutralization; MAbs or MAb combinations that potently neutralized A1 toxin but did not bind A2 toxin had minimal neutralizing capacity for A2 toxin. This was most striking for a combination of three binding domain MAbs which together neutralized >40,000 mouse 50% lethal doses (LD(50)s) of A1 toxin but less than 500 LD(50)s of A2 toxin. Combining three MAbs which bound both A1 and A2 toxins potently neutralized both toxins. We conclude that sequence variability exists within all toxin serotypes, and this impacts monoclonal antibody binding and neutralization. Such subtype sequence variability must be accounted for when generating and evaluating diagnostic and therapeutic antibodies. 相似文献
3.
Overexpression of decoy receptor 3 in precancerous lesions and adenocarcinoma of the esophagus 总被引:13,自引:0,他引:13
Li H Zhang L Lou H Ding I Kim S Wang L Huang J Di Sant'Agnese PA Lei JY 《American journal of clinical pathology》2005,124(2):282-287
Overexpression of decoy receptor (DcR) 3 protein, a recently discovered member of the tumor necrosis factor receptor superfamily, was examined in 40 esophagogastrectomy specimens containing areas of Barrett esophagus (n = 27), low-grade dysplasia (n = 27), high-grade dysplasia or carcinoma in situ (n = 22), and esophageal adenocarcinoma (EAC; n = 28) with immunohistochemical analysis. The results revealed significantly more overexpression of DcR3 in high-grade dysplasia or carcinoma in situ and EAC than in benign esophageal mucosa (both P < .0001), Barrett esophagus (both P < .001), and low-grade dysplasia (P < .01 and P = .033, respectively). Low-grade dysplasia also showed significant overexpression of DcR3 compared with benign esophagus (P < .05) but not with Barrett esophagus (P > .05). DcR3 overexpression seems to negatively correlate with the grade of EAC. Our results suggest that overexpression of DcR3 protein might aid in the diagnosis of high-grade dysplasia or carcinoma in situ and EAC and also might serve as a potential therapeutic target. 相似文献
4.
重组人神经营养因子-4/5蛋白抗三氧化二砷神经毒作用 总被引:5,自引:0,他引:5
目的初步观察重组人神经营养因子-4/5(hNT-4/5)蛋白对三氧化二砷毒性的抑制作用。方法利用hNT-4/5蛋白具有抗神经毒性的特点,采用本实验室克隆表达及部分纯化的具有天然hNT-4/5蛋白生物学活性的重组hNT-4/5蛋白,以不同水平的重组hNT4/5蛋白(0-100μl)与不同浓度的As2O3(0-160μmol/L)同时加入各组鸡胚前脑神经细胞和PC12细胞培养液中共同孵育24-48小时,观察其对染毒鸡胚前脑神经细胞存活和PC12细胞突起生长的影响作用。结果在鸡胚前脑神经细胞和PC12细胞中与As2O3共同培养48小时后,对照组与实验组的细胞存活率差异有显著性,而且细胞存活率和突起数目随hNT-4/5浓度增高而提高和增加。结论初步观察到重组hNT4/5蛋白具有抑制As2O3的毒性作用,为从基因工程途径寻找抗环境毒物因子提供了依据。 相似文献
5.
6.
Calcium/calmodulin-dependent protein kinase II (CaMK II) has been shown to be involved in the regulation of opioid receptor signaling. The present study showed that acute morphine treatment significantly increased both Ca2+/calmodulin-independent and Ca2+/calmodulin-dependent activities of CaMK II in the rat hippocampus, with little alteration in the protein level of either alpha or beta isoform of CaMK II. However, chronic morphine treatment, by which rats were observed to develop apparent tolerance to morphine, significantly down-regulated both Ca2+/calmodulin-independent and Ca2+/calmodulin-dependent activities of CaMK II and differentially regulated the expression of alpha and beta isoforms of CaMK II at protein and mRNA levels. Application of naloxone or discontinuation of morphine treatment after chronic morphine administration, which induced the withdrawal syndrome of morphine, resulted in the overshoot of CaMK II (at both protein and mRNA levels) and its kinase activity. The phenomena of overshoot were mainly observed in the beta isoform of CaMK II but not in the alpha isoform. The effects of both acute and chronic morphine treatments on CaMK II could be completely abolished by the concomitant application of naloxone, indicating that the effects of morphine were achieved through activation of opioid receptors. Our data demonstrated that both acute and chronic morphine treatments could effectively modulate the activity and the expression of CaMK II in the hippocampus. 相似文献
7.
Personalized aspheric intraocular lens implantation based on corneal spherical aberration: a review
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With the evolution of cataract surgery from visual rehabilitation to refractive surgery, aspheric intraocular lenses (IOLs) are being increasingly used in the field of ophthalmology. This increased use can be attributed to negative or zero spherical aberrations with unique optical designs, which counteract some of the positive spherical aberrations of the cornea. These alterations reduce the total spherical aberration of human eyes and improve the visual acuity in patients with cataract postoperatively. At present, various types of aspheric IOLs are used worldwide. Although the implantation of aspheric IOL is beneficial to the patients who need correction of spherical aberrations, much controversy is still associated with ocular residual spherical aberrations that facilitate the best visual quality for patients postoperatively. In order to provide reference for future clinical work and scientific research, this report reviews the relationship between the ocular residual spherical aberration of human eyes and visual quality. 相似文献
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10.
Prostaglandin E(1) protects human liver sinusoidal endothelial cell from apoptosis induced by hypoxia reoxygenation 总被引:14,自引:0,他引:14
Yang H Majno P Morel P Toso C Triponez F Oberholzer J Mentha G Lou J 《Microvascular research》2002,64(1):94-103
Hepatic ischemia-reperfusion injury is an important cause of graft dysfunction after liver transplantation. Liver sinusoidal endothelial cells (LSECs) are particularly sensitive to ischemia-reperfusion injury and undergo apoptosis. This study investigates the protective role of PGE(1) on apoptosis of LSEC during hypoxia-reoxygenation in vitro. Hypothermia-hypoxia followed by reoxygenation triggered LSEC apoptosis, and prostaglandin PGE(1) protected LSEC from apoptosis in a dose-dependent manner. The release of matrix metalloproteinases (MMPs) and nitric oxide (NO) by LSECs were increased after hypoxia reoxygenation. Both the MMP inhibitor BB3103 and the NO inhibitor LNAM effectively decreased LSEC apoptosis, suggesting a separate role of MMPs and NO in hypoxia-reoxygenation-induced LSEC apoptosis. PGE(1) down-regulated NO production by inhibiting the expression of inducible NO synthase in LSEC. PGE(1) also inhibited MMP-2 release from LSEC during hypoxia reoxygenation. These results indicate that the protection of LSECs from apoptosis by PGE(1) in hepatic ischemia-reperfusion injury is mediated by inhibiting inducible NO synthase and MMP release. 相似文献