解耦联蛋白2对Siha细胞辐射敏感性的影响

目的探究沉默解耦联蛋白2(UCP2)联合辐照对Siha细胞辐射敏感性的影响,为宫颈癌放疗增敏提供新的靶点。方法将UCP2 siRNA转染入Siha细胞后进行X射线照射,通过集落形成、CCK-8、流式细胞实验来验证UCP2对Siha细胞辐射敏感性作用,检测线粒体膜电位和活性氧(ROS)的产生来进一步探讨相关机制。结果RT-PCR和Western blot表明Siha细胞经辐照后UCP2表达增加。成功构建UCP2沉默模型,沉默组(siUCP2)D0、Dq、N、SF2分别为1.54、1.31、2.31 Gy和0.52,空白对照组(mock)分别为2.50、3.64、4.30 Gy和0.83,阴性对照组(siNC)分别为3.34、2.16、1.91 Gy和0.69,沉默组较空白对照组和阴性对照组放射增敏比分别为0.62和0.46,并且沉默组细胞增殖活性较对照组明显降低(t=13.2,P<0.05);辐照后沉默组细胞凋亡水平显著高于对照组(t=3.14,P<0.05);照射后12 h,沉默组的ROS产量明显高于对照组(t=19.10,P<0.05);照射后24 h,沉默组的Siha细胞线粒体膜电位较对照组显著降低(t=8.87,P<0.05)。结论UCP2沉默后的Siha细胞辐射敏感性增强,并且可能会成为宫颈癌细胞辐射增敏的新靶点。     

当妇在妇科临床中的应用

当归味甘辛,微苦温,入肝、心、脾经,具有补血活血、调经止痛、润肠通便的功能,常用于血虚,为妇科临床之要药,如月经不调、痛经、经闭、产后腹痛、不孕证等无不适宜。一、月经不调月经先期:中气虚弱、统摄无权、冲任不固、经血失统者,常与补气健脾药配伍,如补中益气汤;肝郁化火、热迫血行所致者,常与     

河南省支气管哮喘诊治现状调查分析

目的：了解河南省哮喘诊治现状，改进防治对策。方法：对2000年河南省全人群采用分层随机整群抽样调查，依据询问病史体检，确诊哮喘患者。结果：受检人口65033人，哮喘患病率1．05％，延误7天以上就诊者40．00％，诊断率为20．00％，诊断方法单一，治疗管理混乱，仅0．30％的哮喘病人按全球哮喘防治创议（GINA）要求正规治疗管理。结论：向基层宣传普及GINA知识是哮喘防治取得成功的重要措施。     

人工流产术病人的心理护理

[目的]了解人工流产病人的心理状态,探讨其心理护理对策.[方法]根据受术者所处的心理状态有针对性地进行综合心理护理.[结果]经精心护理,受术者手术顺利进行.[结论]心理护理有助于缓解人工流产者的情绪,利于手术进行.     

手术室优化护理对预防妇产科开腹手术切口感染的影响

目的：探讨不同护理方式对预防妇产科开腹手术切口感染的影响。方法：选取204例患者随机分为常规护理组和优化护理组,各102例,常规护理组给予手术室常规护理,优化护理组在常规护理的基础上给予优化护理管理。结果：优化护理组患者手术切口的甲级愈合率明显优于常规护理组,优化护理组患者的丙级愈合率、切口感染率、住院天数和治疗总费用等均低于常规护理组,差异有统计学意义（ P＜0．05）。结论：手术室采取优化护理措施,可有效预防妇产科开腹手术患者的切口感染,缩短住院时间,和减轻患者负担。     

急性心肌梗塞30例误诊分析

本文对我院90例急性心肌梗塞进行回顾分析,误诊为其它疾病30例,误诊率30％。其中误诊为消化道疾病6例,呼吸道疾病5例,感染性休克3例,脑血管疾病4例,急性左心衰8例;误诊为牙病、咽炎2例,还有误诊为糖尿病性昏迷、心律失常等2例。为引起临床医生的注意,现分析如下:     

老年人脑卒中早期康复介入疗效观察

Background:Early rehabilitative inervention is essential for improving quality of life in elders with stroke.32 patients received early rehabilitative intervention.We analyzed effect of early rehabilitative intervention in improving functional recovery,preventing and reducing disability. Objective:To investigate effect of early rehabilitative intervention on nerve function in elders with stroke. Unit:Intraneura Department,Tanghai County Hospital,Hebei Province. Subjects:62 patients(36 males,26 females,aged 60～ 78,mean age:70.4) were recruited from intraneural department during January,1999 ～ January 2000.All patients were confirmed by skull CT.Diagnosis was done according to diagnostic standards issued in 4th national cerebrovascular disease meeting.All patients experienced first onset of cerebrovascular disease.34 patients suffered from brain infarction,28 from brain hemorrhage.All patients were randomly divided into two groups,rehabilitation group(n=32),control group(n=30).There were no obvious differences in disease type,sex and age. Intervention:Two groups received routine drug therapy and nonoperational thrombolysis management.Rehabilitation management was initiated in rehabilitation group 48 hours after stable disease status was obtained.In the early phase,good limbs position training in bed,body position exchanges,limbs and passive movement,shift and turning over were carried out.Anti-resistant movement was performed from distal end of limbs to near end.Patients were helped to sit,stand and walk,once per day,40 min /once. Above movement was performed under direction of staffs.Acupuncture and physical therapy were also conducted. Main prognosis indexes:Fugl-meyer scoring was used to evaluate motor function before treatment and 2 months after treatment.Modified Barthel index was used to evaluate ADL. Results:Fugl-meyer score was (32.7± 23.2)and (66.2± 29.8) in rehabilitation group before and after treatment respectively.For control group,Fugl-meyer score was (31.9± 23.1) and (40.3± 23.2) before and after treatment respectively.ADL score was (28.2± 11.9) and (40.5± 8.7) in rehabilitation group before and after treatment respectively.In control group, ADL score was (27.2± 11.3) and (30.2± 10.3) in rehabilitation group before and after treatment respectively.Before treatment, two groups showed no significant difference in score,while posttreatment difference was significant(P< 0.05). Conclusion:Early rehabilitative management improves physiological function and quality of life in elders with stroke.     

SMART 原则在手术室实习护生带教中的应用

[目的]探讨SMART原则在手术室实习护生带教中的应用效果.[方法]选取手术室2013年实习护生120人为研究对象,随机分为两组各60人,对照组护生采用传统带教方法,观察组护生采用“SMART”原则进行带教,比较两组实习护生考核情况及对带教方法的满意度.[结果]两组护生总成绩分级差异有统计学意义（P〈0.05）;情感领域、认知领域以及总成绩比较差异有统计学意义（P〈0.05）;观察组护生对带教方法的满意度高于对照组（P〈0.05）.[结论]采用SMART原则带教具有目标清晰、可量化、切实可行等优点,可以提升实习护生的情感领域及认知领域能力,提高实习护生对带教方法的满意度.     

易理针法治疗网球肘50例临床观察

笔者采用易理针法治疗网球肘50例,收效显著,现报导如下: 一、一般材料:本组50例中,男性11例,女性39例,年龄最大者58岁,小者30岁,病程最短者1个月,最长者2年,大部分接受过针灸、电疗和封闭治疗。二、治疗方法:采用易理针法上下左右交插取穴,嘱患者屈膝坐位,左上肢肘部疼针右下肢阴谷穴,反之亦然,用28号针快速透皮,轻捻转慢进针,得气后,一边提插捻转,一边令患者活动患肢。留针30-60分钟,隔10分钟捻转行针一次。每日一次,5次为一疗程。三、效果观察:60例中,经治疗患者无疼痛,活动自如者为治愈32例,患者活动明显改善,仅感轻微疼痛者为显效13例,中断     

信息化系统在手术患者门前交接首接流程中的应用

目的探讨信息化系统在手术患者门前交接流程中的应用效果。方法随机选取2009年6月30日手术患者60例(对照组)与2009年7月2日手术患者60例(实验组),利用医院信息网络"手术室管理系统"软件,通过条码手腕带对手术患者身份、手术方式及手术部位等进行确认,比较2组患者交接所花时间、手术室及病房护士的满意度。结果平均交接时间由原来的5.2min减少到3.5min,P<0.05,差异有统计学意义。病房护士满意度由78%上升到100%,手术室护士满意度由87%上升到98%。结论信息化系统的应用提高了手术室门前交接的效率和质量,有效保证了对各种手术病人进行快速准确的识别,实现了交接班标准化、流程化、规范化、准确化、信息化管理,减少了护理风险的发生,确保了手术病人的安全。