偏瘫康复胶囊对大鼠静脉血栓形成及出凝血时间的影响

1 实验材料 偏瘫康复胶囊:由黑龙江省康复技术开发公司保健药厂提供,浓度为3g生药/g内容物。消栓再造丸:哈尔滨中药二厂生产,批号:950912。昆明种小白鼠,体重19～21g;Wistar大白鼠,体重160～200g,均由本所动物室提供。统计学分析采用t、t′检验,由孙瑞元提供新药药理统计程序(NDST程序)。2 实验方法与结果2.1 对大鼠静脉血栓形成的影响 实验方法参照文献进行。结果见表1。表1 对大鼠静脉血栓形成的影响(X±S)     

非胰岛素依赖糖尿病自身抗体的胰岛细胞功能的关系

目的 探讨非胰岛素依赖糖尿病（NIDDDM）三种主要自身抗体与胰岛细胞分泌功能之间的关系。方法 对110例正常者和175例初诊为NIDDM的患者测定空腹及口服葡萄糖75克后2小时静脉血浆葡萄糖，胰岛素，C肽，胰高血糖素（分别简写为FPG、2hPG、Fins、2hIns、FCP、2hCP、FGCG、2hGCG），并计算其胰岛素敏感指数（ISI）。同时，测定NIDDM组血清中谷氨酸脱羧酶抗体（CAD－Ab）、胰岛素自身抗体（IAA）和胰岛细胞抗体（ICA），根据三种自身抗体再把NIDDM组分为全阴性、全阳性、岛素自身抗体（IAA）和胰岛细胞抗体（ICA），根据三种自身抗体再把NIDDM组分为全阴性、全阳性、GAD－Ab阳性、IAA阳性、ICA阳性5个亚组。观察初诊NIDDM组自身抗体的阳性率和自身抗体与胰岛细胞功能的关系。结果 （1）NIDDM组GAD－Ab、IAA、ICA阳性率分别为29.1%（51／175）、31.4%(55/175)、18.3%(32/175）。（2）NIDDM组FCP、FGCG比对照组分泌增多，ISI降低（P分别为0.008、＜0.001、＜0.001）。（3）三种抗体全阳性、GAD－Ab阳性、IAA阳性、ICA阳性亚组的FCP均你于全阴性亚组（P＜0.01）。其中全阳性亚组、GAD阳性和ICA阳性亚组的FGCG高于全阴性亚组（P分别为0.007、0.047、0.012）。结论 在初诊的NIDDM患者中自身抗体阳性预示其胰岛B细胞功能受损。     

华盛顿州独立式急救中心的一次调查

早在1970年,随着所谓“独立式急救中心”这类诊所的不断增多,对其争论亦开始出现。虽然公众对于独立式急救中心(Frees     

心安胶囊治疗股骨头缺血性坏死的疗效观察

1临床资料本组共120例,门诊病人88例,住院病人32例,其中男52例,女68例;年龄20～40岁42例,41～50岁38例,50～60岁40例。随机分为治疗组与对照组各60例。两组比较,无明显差异(P<0.05)。诊断标准:依据《中药新药治疗股骨头无菌性坏死的临床研究指导原则》[1]及Ficat分类[2]。2治疗     

FasL基因表达对缺氧状态下直肠癌细胞增殖凋亡的影响

Objective To elucidate the effect of FasL gene expression on the proliferation and apoptosis of hypoxic rectal carcinoma cells. Methods The normoxic expression level of FasL in HR-8348 subtype cells (HR-8348B, HR-8348L, HR-8348F and HR-8348As) with different invasive power were verified by Western blot. Hypoxia models for HR-8348B, HR-8348L, HR-8348F and HR-8348As were constructed with chemical modeling, then the FasL levels in all groups at 12 h after hypoxia were quantitated by Western blot. Distribution of different cell life cycles was determined with flow cytometry. Cell reproductive activities were detected with MTT method, and cell apoptesis was assessed with TUNEL. Results FasL protein was pigmentized at the position of 40 000 by Western blot, and the expression level of FasL was significantly higher in HR-8348F cells than those in HR-8348B, HR-8348L and HR-8348As cells(F=361.149, P<0.01) in normoxia. At 12 h after hypoxia, the FasL level was also significantly higher in HR-8348F cells than those in other groups (F=278.766, P<0.01), but was not markedly different as compared to themselves in normoxia (t=1.762, P>0.05). The proliferation index was significantly higher in HR-8348F (60.43±3.72) than those in HR-8348B (40.01±3.30), HR-8348L (41.30±4.06) and HR-8348As cells (35.87±4.39), respectively (F=39.477, P<0.01). However, both inhibition rate of proliferation and apoptotic index were remarkably lower in HR-8348F (17.30±1.98 and 13.10±1.04) than those in HR-834B (33.70±4.33 and 21.60±1.31), HR-8348L (34.20±3.92 and 20.10±1.15), and HR-8348As (38.00±4.55 and 23.90±1.23), respectively (F=28.811 and 76.462, respectively, P<0.01). Conclusion The expression enhancement of intracellular FasL in rectal carcinoma in hypoxia can lead to accelerated proliferation and reduced apeptosis of cells, which will promote tumor cells to adapt microenvironmental hypoxia.     

季节指数法在传染病预测中的应用

传染病发生的时间分布具有短期波动、季节性升高、长期变异和一定的周期性。传染病报告制度的建立提供了疫情时间序列的原始数据,为数学模型的建立、疫情的预测奠定了基础。本文试用季节指数法对传染病的未来发展提供变动趋势、变动方向、变动程度等方面的定性和定量化的估测,为传染病的管理决策提供依据。     

β—半乳糖苷酶核酸免疫初探

将带有编码β－半乳糖苷酶基因片段的质粒ｐＳＶ－β－Ｇａｌａｃｔｏｓｉｄａｓｅ通过股四头肌接种小鼠，利用ＥＬＩＳＡ方法检测鼠体内β－半乳糖苷酶获得得表达后刺激机体产生抗β－半乳糖苷酶抗体的情况。     

急性炎症期拔牙322例临床分析

牙拔除术一般不在急性炎症期施行.作者自1993年以来,慎重选择322例患牙,顺利进行了患牙拔除,取得满意效果,现报告如下:1 临床资料1.1 一般情况:本组病例中男188名,女134名;年龄10～62岁,平均年龄28岁.其中合并牙龈、牙周脓肿131例,智齿冠周炎及局限性冠周脓肿100例,局限性根尖炎、齿槽脓肿合并齿槽瘘管82例,外伤后肿胀及炎症期9例.100例智齿冠周炎中2例为上颌阻生,其余类型中上颌为149例,下颌为73例.