Evaluation of periodontal status and cytokine/chemokine profile of GCF in patients with severe congenital neutropenia

Severe congenital neutropenia (SCN) is a primary immunodeficiency characterized by defect in neutrophil count. Increased risk of infections in addition to periodontal problems, such as ulcerations of oral mucosa, gingival inflammation, and rapid loss of attachment are common in the course of the disease. The aim of the present study is to define the causal relationship between the severity of periodontal inflammation and severe congenital neutropenia through identification of cytokine profile in gingival crevicular fluid (GCF). A case–control study was performed in patients diagnosed with SCN and healthy controls. Demographic data, the molecular defect, laboratory work-up were gathered from the hospital registry. Periodontal indices were recorded and GCF samples were analyzed using multiplex analysis for the simultaneous measurements of the particular cytokines and chemokines. The present study included 14 patients and 22 control subjects. Both groups were comparable in terms of age and sex. Severity of gingival inflammation measured by the criteria of Löe was higher in the SCN cases (p < 0.05). Moreover, GCF levels of IFN-α, TNF-α, IL-10, IL-13, IL-15, IL-17, IL-2, IL-7, IL-33, IP-10, MIG, MIP-1β were significantly higher in the controls. Decreased cytokine secretion seems to correlate with the decrease in neutrophil counts. The severity of gingival inflammation in SCN patients may be due to the bacterial overgrowth and the change in the content of the oral flora due to the decreased neutrophil counts. Therefore, regular periodontal examinations, the motivation of oral hygiene as well as the compliance with therapy in SCN patients contribute to the periodontal health.

     

Electronic media use by children in families of high socioeconomic level and familial factors

The aim of this research was to describe electronic media access and use among children aged 6 months to 15 years and to identify familial factors. Data was collected with a questionnaire from 724 parents of children seen in the pediatric outpatient clinic of a private hospital while they awaited examination. Questions included electronic media ownership at home or in the child's bedroom, household habits of electronic media use, educational and occupational status of parents, smoking and alcohol consumption at home, and family income. The top three electronic media devices present at home were television, computer and DVD. On a typical day, 32% of children watched television for approximately less than 1 hour, 36% for 2 hours and 22% for 3 hours. Mean television viewing time was 1.89 +/- 0.76 hours. Nearly 12% of parents spent less than 30 minutes with their children, whereas 28% spent 1 hour, and 59% more than 1 hour. Older children spent more time watching television than the younger ones. Forty-six percent of children used computer and the internet at home on a typical day. Almost 20% of children also had a television in their bedroom and spent more time watching television than those without a television in their bedroom, and the ratio increased by age (one-fifth of 3-5-year olds, one-fourth of 6-10-year-olds and half of 11-15-year-olds, on average). The present study provides data for the education and counselling of parents about the use of electronic media by children and it will contribute to increasing the awareness and sensitivity of the population by drawing attention to the subject.     

Risk Factors Affecting Graft and Patient Survivals After Transplantation From Deceased Donors in a Developing Country: A Single-Center Experience

Aim

The aim of this study was to evaluate risk factors affecting graft and patient survival after transplantation from deceased donors.