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Mapping of α- and β-globin genes on Antarctic fish chromosomes by fluorescence in-situ hybridization
Eva Pisano Ennio Cocca Federico Mazzei Laura Ghigliotti Guido di Prisco H. William Detrich III Catherine Ozouf-Costaz 《Chromosome research》2003,11(6):633-640
The pathways and mechanisms of genomic change that have led to the peculiar haemoglobinless phenotype of the white-blooded
Antarctic icefishes (16 species in the family Channichthyidae) constitute an important model for understanding the rapid diversification
of the Antarctic notothenioid fish flock. To provide complementary structural information on genomic change at globin-gene
loci in Antarctic fish species, cytogenetic studies and in-situ chromosomal mapping have been undertaken. Using a DNA probe containing one α- and one β-globin gene from the embryonic/juvenile
globin gene cluster of the red-blooded species Notothenia coriiceps, we mapped the cluster on the chromosomes of Antarctic teleosts by fluorescence in-situ hybridization. As anticipated on the basis of its molecular organization, the cluster was located on a single chromosome
pair in all of the red-blooded fish species probed (N. coriiceps, N. angustata, Trematomus hansoni, T. pennellii). In contrast, the α/β-globin probe did not recognize complementary sequences on the chromosomes of the white-blooded species
Chionodraco hamatus and Channichthys rhinoceratus. These results represent the first example of chromosomal mapping of embryonic/juvenile globin genes in teleostean fishes.
Beyond its relevance to the evolutionary history of Antarctic notothenioids, this work contributes to our understanding of
the evolution of the chromosomal loci of globin genes in fishes and other vertebrates.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Ralls PW; Johnson MB; Kanel G; Dobalian DM; Colletti PM; Boswell WD Jr; Radin DR; Halls JM 《Radiology》1986,161(2):451-454
FM sonography - a signal-processing technique that uses frequency and phase information as well as amplitude data - shows promise in evaluation of patients with diffuse liver disease. In a prospective blinded review of 37 patients with biopsy-proved liver disease and 42 healthy volunteers, FM sonography was clearly superior to traditional amplitude-based (AM) sonography in distinguishing healthy from diseased subjects. Statistically significant differences were seen in accuracy (FM, 98.7%; AM, 84.8%), sensitivity (FM, 97.3%; AM, 70.3%), and negative predictive value (FM, 97.7%; AM, 78.8%). Our data also suggest that current FM sonographic techniques cannot differentiate among histologic findings associated with different hepatic parenchymal abnormalities. It is unclear, therefore, whether FM imaging can reduce the numbers of patients who require biopsy for diagnosis or the frequency of biopsy procedures in patients with known disease. 相似文献
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