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Lucas Siqueira Pinheiro Júlia Eick Iglesias Daiana Boijink Letícia Boldrin Mestieri Patrícia Maria Poli Kopper José Antônio de Poli Figueiredo Fabiana Soares Grecca 《Journal of endodontics》2018,44(7):1140-1145
Introduction
The aim of this study was to evaluate the cell viability and tissue reaction of NeoMTA Plus (NMP; Avalon Biomed Inc, Houston, TX) compared with mineral trioxide aggregate (MTA; Angelus, Londrina, PR, Brazil) and Biodentine (BD; Septodont, Saint-Maur-de-Fossés, France).Methods
Fibroblasts (3T3) were plated and exposed to 1% extract from the test material before and after setting. Cytotoxicity assessment was performed using the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide and sulforhodamine B assays. In vivo evaluation consisted of polyethylene tube implantation of the materials in rat subcutaneous tissue. Histologic analysis occurred at 7, 30, and 90 days, scoring inflammatory events and collagen fiber formation. Analysis of variance and the Tukey and t tests were used for cytocompatibility assays, and the Kruskal-Wallis test followed by the Dunn test were used for biocompatibility assays (P ≤ .05).Results
The materials in the cytotoxicity assays presented greater viability after setting (P ≤ .05). NMP and MTA presented higher viability than the control (Dulbecco modified Eagle medium) on the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay before and after setting (P ≤ .05). The sulforhodamine B assay showed that MTA and BD presented less viability than NMP and the control, and NMP was similar to the control before setting. After setting, MTA and BD presented higher viability when compared with the control group (P ≤ .05), and NMP was similar to control. Inflammatory infiltrate reduction occurred throughout the test periods for all materials. At 7 days, neutrophils were present in BD (P ≤ .05), and granuloma and giant cells were present in BD and MTA. At 30 days, BD showed intense inflammatory infiltrates and a large number of macrophages when compared with NMP, MTA, and the control (P ≤ .05). At 90 days, BD presented a thick fiber layer compared with NMP (P ≤ .05).Conclusions
NMP showed similar biocompatible behavior to MTA and BD. 相似文献3.
Etiele M Gomes Endreo A P dos Santos Leonel F L Neto Danielle R Padilha Gabrielle C S Campos Roberto Thiesen Ingrid R L Machado Maria Ligia A Mestieri 《Journal of the American Association for Laboratory Animal Science》2021,60(5):502
The goal of this study was to develop a method for ultrasound-guided percutaneous intraovarian injection in Wistar rats. Intraovarian administration of chemicals or needle aspiration of the ovary has been undertaken in some species, including humans, equines, and bovines. In rodents, which are widely used in scientific research, a technique for intraovarian injection without surgical exposure of the organ has not been described. The current study standardized the procedure of ovarian percutaneous injection of 0.9% sodium chloride guided by ultrasound in rats. The ovaries were measured by ultrasound imaging before and immediately after injection and showed a significant increase in ovarian length but not width. No clinical abnormalities were detected within 15 d after injection. These findings indicate that the steps of ultrasound localization of the organ, digital restraint, and correct needle insertion achieved successful intraovarian administration of saline without invasive surgery. These results document the feasibility of ultrasound-guided intraovarian percutaneous injection in rats and may be useful for future research on female reproduction and chemical sterilization.Percutaneous injections guided by ultrasound are widely used clinically and for research.1 However, the procedure can be challenging for ovarian injections due to the organ''s location in the abdominal cavity, its size, and its mobility during needle insertion. Transvaginal ultrasound is used to guide intraovarian injections in humans, equines, and bovines, enabling a wide range of procedures to promote or prevent reproduction, including studies with chemical sterilants for animal population control.2,3,5,6 However, in rodents, felines, and canines, little information is available on percutaneous ovarian injections, and surgical procedures are necessary to reach the ovaries.13,14 The development of a minimally invasive technique for ovarian injection would support research and the use of chemical sterilants in females. Because rodents are often used as experimentally to study reproduction, a technique to permit nonsurgical access to rat ovaries would be useful.This study describes ultrasound-guided percutaneous intraovarian injection in rats. The availability of a percutaneous approach to rat ovaries may facilitate future research on reproduction. 相似文献
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Human dental pulp cells response to mineral trioxide aggregate (MTA) and MTA Plus: cytotoxicity and gene expression analysis 
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Marla Cuppini Kelly Cristine Zatta Letícia Boldrin Mestieri Fabiana Soares Grecca Vicente Castelo Branco Leitune Sílvia Stanisçuaski Guterres Fabrício Mezzomo Collares 《Dental materials》2019,35(3):457-467
Objective
The aim of this study was to synthesize and characterize an experimental endodontic paste.Methods
An experimental endodontic paste (EX) was characterized by its particle size, zeta potential, drug content and morphology. The powder of EX is composed of amoxicillin microspheres, calcium tungstate and α-tricalcium phosphate, mixed with an indomethacin nanocapsules suspension. Ultracal® (Ultradent), an iodoform-based paste (GP) and the EX were evaluated by its physical properties (flow, film thickness and radiopacity). The cytocompatibility was performed by MTT and SRB-colorimetric assays; the cell-migration was tested with scratch assay and cell-ability to remineralization with ALP and Alizarin Red S, with fibroblastic cell line. The antibacterial activity was assessed by the formation of inhibition zones and against planktonic bacteria.Results
The EX and UL flow achieved ISO6876 standard, and GP was lower than 17 mm. All pastes achieved the film thickness required. Radiopacity was equivalent to 1.81 ± 0.25 mmAl for EX, which did not differ from GP group 1.39 ± 0.33 mmAl (p > 0.05). The UL presented 3.04 ± 0.33 mmAl. The values for SRB showed better citocompatibility in comparison with MTT for all materials. The ALP activity and formation of mineralized nodules demonstrated the remineralization potential for UL and EX. Cell migration showed continuous wound closure until complete cell healing, however, the EX accelerated the process (p < 0.05). The EX showed the greatest inhibition zone (p < 0.05) and was the only group with antibacterial activity against planktonic bacteria.Significance
The synthesized endodontic paste demonstrated reliable physical and biological properties and could be a promising material for periapical tissue repair. 相似文献8.
Delai Débora Jardine Alexander Pompermayer Mestieri Letícia Boldrin Boijink Daiana Fontanella Vania Regina Camargo Grecca Fabiana Soares Kopper Patrícia Maria Poli 《Clinical oral investigations》2019,23(4):1837-1844
Objectives
This study aimed to compare WaveOne Gold with ProTaper and RaCe systems regarding remaining filling material, apical transportation (AT), and working time (WT) after (i) filling removal and (ii) shaping of curved canals.
MethodsThirty mesiobuccal canals of maxillary molars were prepared and filled. After 30 days, they were randomly assigned into three groups (n = 10), according to the instruments used for filling removal and shaping, respectively: WOG—WaveOne Gold Primary and Medium; PTG—ProTaper Retreatment and ProTaper Next; RCG—D-RaCe and RaCe. Micro-CT analysis assessed the residual filling material and AT. WT was recorded. Data were statistically analyzed (α = .05).
ResultsThere was no significant difference between groups in the amount of filling material at any evaluated moment (P > .05). All groups presented low AT values. The WT was similar in all groups in filling removal (P > .05), and in shaping step WOG was faster than PTG and RCG (P < .05).
ConclusionsNeither system could completely remove the filling material. The instruments evaluated were safe and the reciprocating system was faster than the rotary systems in shaping the canals.
Clinical relevanceThis study provided consistent information on filling material removal capacity of WaveOne Gold. Considering that all tested systems were safe, WaveOne Gold may be an alternative with cost-effectiveness and shorter learning curve for endodontic retreatment.
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