全文获取类型
收费全文 | 6828篇 |
免费 | 364篇 |
国内免费 | 46篇 |
专业分类
耳鼻咽喉 | 114篇 |
儿科学 | 300篇 |
妇产科学 | 167篇 |
基础医学 | 618篇 |
口腔科学 | 248篇 |
临床医学 | 647篇 |
内科学 | 1552篇 |
皮肤病学 | 132篇 |
神经病学 | 366篇 |
特种医学 | 154篇 |
外科学 | 1012篇 |
综合类 | 377篇 |
一般理论 | 5篇 |
预防医学 | 467篇 |
眼科学 | 198篇 |
药学 | 519篇 |
中国医学 | 46篇 |
肿瘤学 | 316篇 |
出版年
2024年 | 5篇 |
2023年 | 94篇 |
2022年 | 264篇 |
2021年 | 487篇 |
2020年 | 229篇 |
2019年 | 268篇 |
2018年 | 283篇 |
2017年 | 205篇 |
2016年 | 232篇 |
2015年 | 295篇 |
2014年 | 340篇 |
2013年 | 347篇 |
2012年 | 527篇 |
2011年 | 515篇 |
2010年 | 274篇 |
2009年 | 245篇 |
2008年 | 384篇 |
2007年 | 355篇 |
2006年 | 348篇 |
2005年 | 341篇 |
2004年 | 278篇 |
2003年 | 249篇 |
2002年 | 201篇 |
2001年 | 52篇 |
2000年 | 57篇 |
1999年 | 42篇 |
1998年 | 33篇 |
1997年 | 19篇 |
1996年 | 24篇 |
1995年 | 16篇 |
1994年 | 16篇 |
1993年 | 8篇 |
1992年 | 23篇 |
1991年 | 20篇 |
1990年 | 23篇 |
1989年 | 15篇 |
1988年 | 15篇 |
1987年 | 14篇 |
1986年 | 6篇 |
1985年 | 12篇 |
1984年 | 6篇 |
1983年 | 10篇 |
1980年 | 5篇 |
1979年 | 6篇 |
1977年 | 5篇 |
1974年 | 6篇 |
1973年 | 5篇 |
1972年 | 5篇 |
1970年 | 5篇 |
1969年 | 4篇 |
排序方式: 共有7238条查询结果,搜索用时 140 毫秒
1.
2.
3.
Expression of TRIM22 mRNA in chronic hepatitis C patients treated with direct-acting antiviral drugs
Mariam Naveed Amjad Ali Nadeem Sheikh Shazia Rafique Muhammad Idrees 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2020,128(4):326-334
Hepatitis C is a global public health problem, and Pakistan is the second largest country in the globe with highest prevalence rate of hepatitis C virus (HCV). Until 2014, pegylated interferon (PEG-IFN) plus ribavirin (RBV) has been the standard therapy for HCV, however, owing to its adverse side effects and very low sustained virologic response (SVR) rates therapeutics trend is shifted toward direct-acting antivirals. Tripartite motif containing 22 (TRIM22) is a dynamic antiviral protein that can inhibit multiple viruses in vivo. Expression of TRIM22 mRNA has been linked to outcome of PEG-IFN and ribavirin therapy, where its higher expression leads to rapid virus clearance. However, in terms of therapy with direct-acting antiviral (DAA) or double DAA, impact of TRIM22 expression is largely unknown. These new drugs show more than 90% of SVR rates and lesser side effects and have proven to be better than IFN therapy. Endogenous IFN system suppresses various pathogens through the induction of antiviral effectors termed as interferon-stimulating genes (ISGs). We have studied the expression levels of one of these antiviral effectors, TRIM22 in response to sofosbuvir (SOF) and daclatasvir (DAC) in combination with RBV, using quantitative PCR in the peripheral blood mononuclear cells (PBMCs) of HCV-infected patients. We have observed sustained virus clearance in more than 90% of patients treated with DAA and double DAA and have seen the expression of TRIM22 to be higher in patients who attained SVR as compared to the untreated patients. We have also observed downregulation of TRIM22 in patients who failed to attain rapid virus clearance, and upregulation in those who achieved rapid clearance of virus. Genetic factors that determine the lower TRIM22 expression in these patients are needed to be explored that may also play a role in lower response to anti-HCV therapy. Endogenous IFN system and effects of antiviral proteins in response to DAA therapy is needed to be studied in order to better understand the host response toward these drugs to make them more effective. 相似文献
4.
5.
Alexander Stoff MD ; Angel A. Rivera PhD ; N. S. Banerjee PhD ; J. Michael Mathis PhD ; Antonio Espinosa-de-los-Monteros MD ; Long P. Le PhD ; Jorge I. De la Torre MD ; Luis O. Vasconez MD ; Thomas R. Broker PhD ; Dirk F. Richter MD ; Mariam A. Stoff-Khalili MD ; David T. Curiel MD PhD 《Wound repair and regeneration》2006,14(5):608-617
Genetically modified keratinocytes and fibroblasts are suitable for delivery of therapeutic genes capable of modifying the wound healing process. However, efficient gene delivery is a prerequisite for successful gene therapy of wounds. Whereas adenoviral vectors (Ads) exhibit superior levels of in vivo gene transfer, their transductional efficiency to cells resident within wounds may nonetheless be suboptimal, due to deficiency of the primary adenovirus receptor, coxsackie-adenovirus receptor (CAR). We explored CAR-independent transduction to fibroblasts and keratinocytes using a panel of CAR-independent fiber-modified Ads to determine enhancement of infectivity. These fiber-modified adenoviral vectors included Ad 3 knob (Ad5/3), canine Ad serotype 2 knob (Ad5CAV-2), RGD (Ad5.RGD), polylysine (Ad5.pK7), or both RGD and polylysine (Ad5.RGD.pK7). To evaluate whether transduction efficiencies of the fiber-modified adenoviral vectors correlated with the expression of their putative receptors on keratinocytes and fibroblasts, we analyzed the mRNA levels of CAR, alpha upsilon integrin, syndecan-1, and glypican-1 using quantitative polymerase chain reaction. Analysis of luciferase and green fluorescent protein transgene expression showed superior transduction efficiency of Ad5.pK7 in keratinocytes and Ad5.RGD.pK7 in fibroblasts. mRNA expression of alpha upsilon integrin, syndecan-1 and glypican-1 was significantly higher in primary fibroblasts than CAR. In keratinocytes, syndecan-1 expression was significantly higher than all the other receptors tested. Significant infectivity enhancement was achieved in keratinocytes and fibroblasts using fiber-modified adenoviral vectors. These strategies to enhance infectivity may help to achieve higher clinical efficacy of wound gene therapy. 相似文献
6.
7.
8.
Vedat Davutoglu Serdar Soydinc Abdullah Aydin Metin Karakok 《Journal of the American Society of Echocardiography》2005,18(2):185-187
The exposure to Aspergillus organisms/spores is likely common, but disease caused by tissue invasion with these fungi is uncommon and occurs primarily in the setting of immunosuppression. We report a case of rapidly advancing invasive endomyocardial aspergillosis secondary to prolonged usage of multiple broad-spectrum antibiotics in a nonimmunocompromised host. A 36-year-old cotton textile worker presented to our institution with a 3-month history of weight loss and fatigue. He reported receiving prolonged use of multiple broad-spectrum antibiotic treatment. The echocardiogram demonstrated multiple endomyocardial vegetations and a mass in the left atrium. Myocardial biopsy specimen revealed an invasive endomyocardial aspergillosis. The patient was investigated for immune deficiency including HIV, and this workup was negative. Treatment was started with amphotericin B and heparin for presumed left atrial thrombus. The patient died because of a rupture of mycotic aneurysm that resulted in cerebral hemorrhage. This case illustrates the risk of an invasive fungal infection in a nonimmunocompromised host who is a prolonged user of antibiotics in the setting of environmental exposure of opportunistic invasive fungal infections. 相似文献
9.
Chenicheri Balakrishnan Gil Altman Abdullah J Khalil 《CANADIAN JOURNAL OF PLASTIC SURGERY》2005,13(4):209-211
During lower extremity amputation, the objective is to provide a functional residual limb that permits maximum patient mobility and independence. Preservation of length of the fore part of the foot using salvageable tissue from the amputated part in young patients prevents equines deformity and revision of amputation to a higher level. This can be achieved using tissue available from the amputated part. The spare part concept in reconstructive surgery should be integrated into the trauma algorithm to avoid additional donor site morbidity. Reported here is a young adult patient with crush injuries to both feet, which resulted in amputations. A fasciocutaneous flap raised from one extremity was used to facilitate transmetatarsal amputation stump length preservation of the other extremity. 相似文献
10.
Evren Isci Halil Ibrahim Canter Abdullah Kecik 《European journal of plastic surgery》2007,30(1):45-46
Rabbits are the experimental animals of choice in many studies including flaps, wounds, and topical trials for new pharmacological
agents. Because the topical drugs and other materials used for dressing are irritating to the animal, they tend to remove
dressings, bandages, drapes, insensate flaps, and also topical drugs by scratching, licking, and biting if they are within
the reach of the animal. In this study, we report an easy to prepare, user-friendly, comfortable, and cheap dressing protector
called a dressing shield to prevent these problems. 相似文献