Pulpal anesthesia in pediatric patients following supplemental mandibular buccal infiltration in vital permanent mandibular molars with deep caries

Clinical Oral Investigations - Inferior alveolar nerve block (IANB) does not always provide adequate pulpal anesthesia, and supplemental techniques have been investigated in adults. This study...     

Dynamic Irrigation Promotes Apical Papilla Cell Attachment in an Ex Vivo Immature Root Canal Model

Introduction

The use of dentin preconditioning techniques in regenerative endodontic procedures is currently promising. Several growth factors have been detected on dentin after ultrasonic irrigation with EDTA. This study aimed to evaluate the effects of dynamic irrigation with different solution regimens on apical papilla cell (APC) attachment in an ex vivo immature tooth model.

Effect of fluocinolone acetonide on human dental pulp cells: cytotoxicity, proliferation, and extracellular matrix formation

Introduction

The goal of vital pulp therapy is to maintain pulp vitality and function. Fluocinolone acetonide is a potent topical glucocorticoid used in the treatment of skin disorders and oral lesions that could possibly be used to resolve inflammation and stimulate the healing process of inflamed dental pulp. The purpose of this study was to investigate the effects of fluocinolone acetonide (0.1-50 μmol/L) on cytotoxicity, cell proliferation, and fibronectin and type I collagen synthesis in human dental pulp cells (HDPCs).

Methods

HDPCs were prepared from freshly extracted human third molars. MTT assay was used to determine toxicity and cell proliferation. Western blot analysis was performed to detect fibronectin and type I collagen synthesis.

Results

Low concentrations of fluocinolone acetonide were not only nontoxic but also significantly stimulated cell proliferation (P < .05). Fluocinolone acetonide significantly stimulated fibronectin and type I collagen synthesis (P < .05).

Conclusions

Low concentrations (0.1-10 μmol/L) of fluocinolone acetonide might have the potential to stimulate healing of inflamed dental pulp.     

Incorporation of anti-inflammatory agent into calcium hydroxide pulp capping material: an in vitro study of physical and mechanical properties

The aims of this study were to investigate the release of fluocinolone acetonide from an experimental pulp capping material containing fluocinolone acetonide (PCFA) and compare some physical and mechanical properties with Dycal(?). The PCFA is a hard-setting calcium hydroxide cement composed of 50 mmol/L fluocinolone acetonide. Conditioned media from the setting material was collected for determination of fluocinolone acetonide release by high performance liquid chromatography and pH measurement by pH meter. The setting time, compressive strength, disintegration, and acid soluble arsenic content were measured according to ISO 3107:2004. Dycal(?) was used as control. Fluocinolone acetonide could release at a range of suitable concentrations from PCFA. The pH, setting time, and acid soluble arsenic content of PCFA were significantly higher than those of Dycal(?). The compressive strength and disintegration of PCFA were comparable to control. PCFA may be considered as an alternative in pulp capping of inflamed dental pulp tissue.