全文获取类型
收费全文 | 6264篇 |
免费 | 453篇 |
国内免费 | 36篇 |
专业分类
耳鼻咽喉 | 90篇 |
儿科学 | 150篇 |
妇产科学 | 113篇 |
基础医学 | 900篇 |
口腔科学 | 516篇 |
临床医学 | 588篇 |
内科学 | 1346篇 |
皮肤病学 | 173篇 |
神经病学 | 559篇 |
特种医学 | 71篇 |
外科学 | 595篇 |
综合类 | 48篇 |
一般理论 | 1篇 |
预防医学 | 703篇 |
眼科学 | 113篇 |
药学 | 454篇 |
中国医学 | 43篇 |
肿瘤学 | 290篇 |
出版年
2023年 | 70篇 |
2022年 | 119篇 |
2021年 | 286篇 |
2020年 | 183篇 |
2019年 | 236篇 |
2018年 | 307篇 |
2017年 | 196篇 |
2016年 | 224篇 |
2015年 | 250篇 |
2014年 | 319篇 |
2013年 | 346篇 |
2012年 | 542篇 |
2011年 | 592篇 |
2010年 | 305篇 |
2009年 | 230篇 |
2008年 | 349篇 |
2007年 | 405篇 |
2006年 | 286篇 |
2005年 | 271篇 |
2004年 | 225篇 |
2003年 | 195篇 |
2002年 | 166篇 |
2001年 | 72篇 |
2000年 | 72篇 |
1999年 | 58篇 |
1998年 | 29篇 |
1997年 | 13篇 |
1996年 | 21篇 |
1995年 | 14篇 |
1994年 | 19篇 |
1993年 | 11篇 |
1992年 | 20篇 |
1991年 | 18篇 |
1990年 | 13篇 |
1989年 | 10篇 |
1988年 | 14篇 |
1987年 | 11篇 |
1986年 | 19篇 |
1985年 | 14篇 |
1984年 | 13篇 |
1983年 | 12篇 |
1981年 | 8篇 |
1979年 | 8篇 |
1977年 | 10篇 |
1975年 | 8篇 |
1974年 | 15篇 |
1973年 | 13篇 |
1968年 | 7篇 |
1967年 | 11篇 |
1966年 | 7篇 |
排序方式: 共有6753条查询结果,搜索用时 15 毫秒
1.
Prevention Science - This study aimed to evaluate the effectiveness of the drug and violence resistance educational program (PROERD) on short-term secondary outcomes, such as intentions to use... 相似文献
2.
Phenotypic and genetic features of enteropathogenic Escherichia coli isolates from diarrheal children in the Ribeirão Preto metropolitan area,São Paulo State,Brazil
下载免费PDF全文
![点击此处可从《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》网站下载免费的PDF全文](/ch/ext_images/free.gif)
André Pitondo‐Silva Gerson Nakazato Juliana P. Falcão Kinue Irino Roberto Martinez Ana Lúcia C. Darini Rodrigo Tavanelli Hernandes 《APMIS : acta pathologica, microbiologica, et immunologica Scandinavica》2015,123(2):128-135
This study was designed to characterize a collection of 60 enteropathogenic Escherichia coli (EPEC) isolates from diarrheic feces of patients in the Ribeirão Preto metropolitan area regarding different phenotypic and molecular features. We examined antibiotic resistance profiles, occurrence of virulence factors‐encoding genes, intimin subtypes and the correlation of serotypes among typical (tEPEC) and atypical (aEPEC) EPEC isolates. The results demonstrated that atypical EPEC was more heterogeneous than typical EPEC concerning the characteristics investigated and 45.2% do not belong to classical EPEC serogroups. Intimin subtype β was the most frequent among the EPEC isolates (46.7%), being detected in both tEPEC and aEPEC. The majority of aEPEC isolates presented localized adherence‐like (LAL) pattern to HEp‐2 cells, although aEPEC isolates displaying diffuse adherence (DA) or non‐adherent were also detected. High prevalence of antimicrobial resistance was found for ampicillin, cephalothin, sulfonamide and tetracycline. In general, tEPEC isolates were more resistant to the antimicrobials tested than aEPEC isolates. 相似文献
3.
Caroline A. Harrison BMedSci MRCS ; Martin J. Heaton MD FRCS ; Christopher M. Layton PhD ; Sheila Mac Neil PhD 《Wound repair and regeneration》2006,14(2):203-209
To produce a stable epidermis, keratinocytes need to be firmly attached to the basement membrane. However, following wounding, keratinocytes are required to develop a migratory phenotype in order to reepithelialize the wound. To investigate some of the issues underlying reepithelialization, we have developed a three-dimensional in vitro model of tissue-engineered skin, comprising sterilized human dermis seeded with human keratinocytes and dermal fibroblasts. Using this model, we have shown that the inclusion of fibroblasts within the model increases the stability of keratinocyte attachment. We have also demonstrated that keratinocyte migration occurs most effectively in the absence of a basement membrane and following the inclusion of fibroblasts in the model. In addition, subjecting the keratinocyte layer to mechanical trauma induces a migratory phenotype. We conclude that this three-dimensional in vitro wound model can be used to increase our understanding of the factors that enhance keratinocyte migration and hence wound healing in vivo. 相似文献
4.
5.
Bacterial ghosts (BGs) are empty bacterial envelopes of Gram-negative bacteria produced by controlled expression of cloned gene E, forming a lysis tunnel structure within the envelope of the living bacteria. BGs are devoid of cytoplasmic content and possess all bacterial bio-adhesive surface properties in their original state while not posing any infectious threat. BGs are ideally suited as an advanced drug delivery system (ADDS) for toxic substances in tumor therapy. The inner space of BGs can be loaded with either single components or combinations of peptides, drugs or DNA which provides an opportunity to design new types of (polyvalent) drug delivery vehicles. Uptake of BGs loaded with Doxorubicin (Dox) by CaCo2 cells led to effective Dox release from endo-lysosomal compartments and accumulation in the nucleus. Viability and proliferative capacity of the cells were significantly decreased (2–3 orders of magnitude) after internalization of Dox loaded BGs as compared to cells incubated with free Dox. The same effect was observed with leukemia cells. Melanoma cells also revealed a high capability to internalize BGs. These results indicate that BGs are able to target a range of types of cancer. BGs have also been investigated as DNA delivery vectors. Studies show DNA loaded BGs are efficiently phagocytosed and internalized by both professional APCs and tumor cells with up to 82% of cells expressing the plasmid-encoded reporter gene. Our studies with BGs as an ADDS system contribute (i) to optimize drug delivery for the treatment of cancer; (ii) define specific conditions for selection and preparation of BG formulations; (iii) and provide a background for the clinical application of BGs in cancer therapy. 相似文献
6.
Carlos Ferrer Albiach Antonio Conde Moreno Marta Rodríguez Cordón Virginia Morillo Macías Ana Bouché Babiloni Inmaculada Beato Tortajada Ángel Sánchez Iglesias Alicia Francés Muñoz 《Clinical & translational oncology》2010,12(1):22-26
Hypoxia is related to poor prognosis because it is associated to chemo-and radioresistance. During recent years the evolution
of imaging methods like PET/CT and MRI has meant the appearance of new perspectives with direct implications in radiation
therapy. We discuss previous experiences in staging, planning and in the follow-up process with these techniques for measuring
tumour hypoxia. 相似文献
7.
8.
9.
10.
LIMITATIONS OF RADIOTHERAPY IN THE DEFINITIVE TREATMENT OF SQUAMOUS CARCINOMA OF THE TONSILLAR FOSSA
Christopher J. O'Brien Geeta K. Castle Graham N. Stevens G. Mac Halliday John K. Donovan Kenneth K. Lee Nicholas A. Packham Maurice J. Peat 《ANZ journal of surgery》1992,62(9):709-713
Between 1970 and 1990, 104 patients with squamous cell carcinoma (SCC) of the tonsil were treated. The median age was 58 years and 80% of patients were males. Distribution among clinical stages was: stage I, 19 patients; stage II, 12 patients; stage III, 23 patients; and stage IV, 48 patients. More than 70% of patients had initial radiotherapy as definitive treatment irrespective of stage, reflecting the treatment philosophy over much of this period. The overall survival rate was 26% at 5 years, with survival being significantly affected by T stage, clinical stage and age. Clinical node status did not significantly affect survival rates. Good local control of T1N0 cancers was achieved with radiotherapy alone, but patients with more advanced cancers did poorly. We have now moved away from a non-selective policy and use initial surgery combined with postoperative radiotherapy in most patients, reserving radiotherapy alone for mainly early tonsil cancers. 相似文献