排序方式： 共有4条查询结果，搜索用时 0 毫秒
1
1.
Lucas Siqueira Pinheiro Júlia Eick Iglesias Daiana Boijink Letícia Boldrin Mestieri Patrícia Maria Poli Kopper José Antônio de Poli Figueiredo Fabiana Soares Grecca 《Journal of endodontics》2018,44(7):1140-1145
Introduction
The aim of this study was to evaluate the cell viability and tissue reaction of NeoMTA Plus (NMP; Avalon Biomed Inc, Houston, TX) compared with mineral trioxide aggregate (MTA; Angelus, Londrina, PR, Brazil) and Biodentine (BD; Septodont, Saint-Maur-de-Fossés, France).Methods
Fibroblasts (3T3) were plated and exposed to 1% extract from the test material before and after setting. Cytotoxicity assessment was performed using the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide and sulforhodamine B assays. In vivo evaluation consisted of polyethylene tube implantation of the materials in rat subcutaneous tissue. Histologic analysis occurred at 7, 30, and 90 days, scoring inflammatory events and collagen fiber formation. Analysis of variance and the Tukey and t tests were used for cytocompatibility assays, and the Kruskal-Wallis test followed by the Dunn test were used for biocompatibility assays (P ≤ .05).Results
The materials in the cytotoxicity assays presented greater viability after setting (P ≤ .05). NMP and MTA presented higher viability than the control (Dulbecco modified Eagle medium) on the 3-(4,5-dimethyl-thiazoyl)-2,5-diphenyl-tetrazolium bromide assay before and after setting (P ≤ .05). The sulforhodamine B assay showed that MTA and BD presented less viability than NMP and the control, and NMP was similar to the control before setting. After setting, MTA and BD presented higher viability when compared with the control group (P ≤ .05), and NMP was similar to control. Inflammatory infiltrate reduction occurred throughout the test periods for all materials. At 7 days, neutrophils were present in BD (P ≤ .05), and granuloma and giant cells were present in BD and MTA. At 30 days, BD showed intense inflammatory infiltrates and a large number of macrophages when compared with NMP, MTA, and the control (P ≤ .05). At 90 days, BD presented a thick fiber layer compared with NMP (P ≤ .05).Conclusions
NMP showed similar biocompatible behavior to MTA and BD. 相似文献2.
Daiana Boijink Débora Delai Costa Carolina Bender Hoppe Patrícia Maria Polli Kopper Fabiana Saores Grecca 《Journal of endodontics》2018,44(8):1289-1292
Introduction
The purpose of this study was to evaluate apical debris extrusion associated with different kinematics in curved root canals.Methods
Forty-five extracted mandibular molars with root curvature angles ranging between 20° and 40° and curvature radii <10 mm were randomly assigned to 3 groups (n = 15) according to the kinematics used for root canal preparation: reciprocating motion with the WaveOne Gold system (Dentsply Tulsa Dental, Tulsa, OK), rotary/reciprocating motion with the Twisted File Adaptive system (SybronEndo, Orange, CA), and the manual technique. The debris apically extruded during preparation was collected into preweighed Eppendorf tubes. The tubes were then stored in an incubator at 70°C for 5 days. The extruded debris was quantified by subtracting the preinstrumentation from the postinstrumentation weight of the Eppendorf tubes. The time required for each instrumentation procedure was recorded. Data were analyzed using 1-way analysis of variance Tukey post hoc tests (∝ = 0.05).Results
The WaveOne Gold reciprocating single-file system was associated with less extrusion of debris compared with hand files (P < .05) and the Twisted File Adaptive system (P > .05). The preparation time required by hand files was significantly longer than that required by the other techniques (P < .05).Conclusions
Under the conditions of this study, all of the instrumentation systems caused apical debris extrusion to some degree. The WaveOne Gold reciprocating system was associated with less debris extrusion in curved root canals compared with the manual technique and the Twisted File Adaptive system although the difference between the WaveOne Gold and Twisted File Adaptive systems was not significant. 相似文献3.
Fernanda Garcia Sampaio Cheila de Lima Boijink Laila Romagueira Bichara dos Santos Eliane Tie Oba Ana Lúcia Kalinin Francisco Tadeu Rantin 《Ecotoxicology (London, England)》2010,19(5):963-976
Copper sulfate is widely used in aquaculture. Exposure to this compound can be harmful to fish, resulting in oxidative metabolism
alterations and gill tissue damage. Pacu, Piaractus mesopotamicus, (wt = 43.4 ± 3.35 g) were distributed in experimental tanks (n = 10; 180 l) and exposed for 48 h to control (without copper
addition), 0.4Cu (0.4 mg l−1), 0CupH (without copper addition, pH = 5.0) and 0.4CupH (0.4 mg l−1, pH = 5.0). In liver and red muscle, the superoxide dismutase (SOD) was responsive to the increases in the aquatic copper.
The plasmatic intermediary metabolites and hematological variables in the fish of group 0.4Cu were similar to those of the
control group. Conversely, the exposure to 0.4CupH caused an increase in the plasmatic lactate, number of red blood cells
(RBC) and hemoglobin (Hb). Plasmatic copper concentration [Cup] increased in group 0.4Cu and 0.4CupH, which is higher in group 0.4CupH, suggests an effect of water pH on the absorbed copper.
Exposure to 0.4Cu and 0.4CupH resulted in a reduction in the Na+/K+-ATPase activity and an increase in metallothionein (MT) in the gills. Exposure to 0CupH caused a decrease in glucose and
pyruvate concentrations and an increase in RBC, Hb, and the branchial Na+/K+-ATPase activity. These responses suggest that the fish triggered mechanisms to revert the blood acidosis, save energy and
increase the oxygen uptake. MT was an effective biomarker, responding to copper in different pHs and dissolved oxygen. Combined-factors
caused more significant disturbance in the biomarkers than single-factors. 相似文献
4.
Delai Débora Jardine Alexander Pompermayer Mestieri Letícia Boldrin Boijink Daiana Fontanella Vania Regina Camargo Grecca Fabiana Soares Kopper Patrícia Maria Poli 《Clinical oral investigations》2019,23(4):1837-1844
Objectives
This study aimed to compare WaveOne Gold with ProTaper and RaCe systems regarding remaining filling material, apical transportation (AT), and working time (WT) after (i) filling removal and (ii) shaping of curved canals.
MethodsThirty mesiobuccal canals of maxillary molars were prepared and filled. After 30 days, they were randomly assigned into three groups (n = 10), according to the instruments used for filling removal and shaping, respectively: WOG—WaveOne Gold Primary and Medium; PTG—ProTaper Retreatment and ProTaper Next; RCG—D-RaCe and RaCe. Micro-CT analysis assessed the residual filling material and AT. WT was recorded. Data were statistically analyzed (α = .05).
ResultsThere was no significant difference between groups in the amount of filling material at any evaluated moment (P > .05). All groups presented low AT values. The WT was similar in all groups in filling removal (P > .05), and in shaping step WOG was faster than PTG and RCG (P < .05).
ConclusionsNeither system could completely remove the filling material. The instruments evaluated were safe and the reciprocating system was faster than the rotary systems in shaping the canals.
Clinical relevanceThis study provided consistent information on filling material removal capacity of WaveOne Gold. Considering that all tested systems were safe, WaveOne Gold may be an alternative with cost-effectiveness and shorter learning curve for endodontic retreatment.
相似文献
1