全文获取类型
收费全文 | 497篇 |
免费 | 54篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 37篇 |
妇产科学 | 9篇 |
基础医学 | 40篇 |
口腔科学 | 33篇 |
临床医学 | 50篇 |
内科学 | 159篇 |
皮肤病学 | 9篇 |
神经病学 | 72篇 |
特种医学 | 16篇 |
外科学 | 78篇 |
综合类 | 1篇 |
预防医学 | 7篇 |
眼科学 | 13篇 |
药学 | 8篇 |
中国医学 | 1篇 |
肿瘤学 | 16篇 |
出版年
2023年 | 5篇 |
2022年 | 7篇 |
2021年 | 12篇 |
2020年 | 10篇 |
2019年 | 15篇 |
2018年 | 33篇 |
2017年 | 19篇 |
2016年 | 13篇 |
2015年 | 20篇 |
2014年 | 22篇 |
2013年 | 11篇 |
2012年 | 23篇 |
2011年 | 28篇 |
2010年 | 14篇 |
2009年 | 16篇 |
2008年 | 26篇 |
2007年 | 39篇 |
2006年 | 45篇 |
2005年 | 39篇 |
2004年 | 35篇 |
2003年 | 39篇 |
2002年 | 17篇 |
2001年 | 21篇 |
2000年 | 11篇 |
1999年 | 11篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1992年 | 4篇 |
1991年 | 3篇 |
1990年 | 3篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1975年 | 1篇 |
1968年 | 2篇 |
排序方式: 共有553条查询结果,搜索用时 125 毫秒
1.
Oral Saygun Serdar Topaloglu Fatih M Avsar Hakan Ozel Sema Hucumenoglu Mustafa Sahin Suleyman Hengirmen 《Canadian journal of surgery》2006,49(2):107-112
BACKGROUND: Most duodenal injuries are managed with primary repair, but the degree of duodenal-wall injury may threaten the integrity of the primary repair. Therefore, we evaluated whether the primary repair site could be reinforced with histoacryl glue (HAG) or HAG with an expanded polytetrafluoroethylene (ePTFE) mesh. METHODS: Grade 3 duodenal injury in the second portion of the rat duodenum was chosen as a standard trauma model. Thirty-three male rats were divided into sham (n = 3), 2-layer primary repair (n = 10), 1-layer primary repair plus HAG application (n = 10) and ePTFE attached with HAG over the 1-layer primary repair site (n = 10) groups. Ten-day survival, adhesion grades and histological assessment were taken as outcome measures. RESULTS: A significant survival advantage was identified in the group that had an ePTFE graft attached with HAG over a 1-layer repair when compared with the group that had a 2-layer primary repair. Adhesion grades were found to be particularly increased in the group that had an ePTFE graft attached with HAG over the primary repair site, moderately increased in the primary repair plus HAG application group and lower in the 2-layer primary repair group. ePTFE graft application was found to be beneficial to coverage of the HAG-dependent empty spaces in the serosal layer. CONCLUSIONS: A primary repair site after duodenal trauma or a difficult duodenal stump can be reinforced with the application of HAG or ePTFE graft implantation with HAG. 相似文献
2.
3.
Onder Güney Jale Bengi Celik Mehmet Arazi Mehmet Erkan Ustun 《Journal of clinical neuroscience》2004,11(5):517-520
This study aims to evaluate the effects of gamma-hydroxybutyrate (GHB) after spinal cord trauma (SCT). Twenty rabbits were divided equally into four groups: group I was the sham-operated group, group II suffered from SCT but received no treatment, group III was given a dose of 400 mg/kg of GHB intravenously before SCT and group IV received the same dose after SCT. Cerebrospinal fluid (CSF) samples were obtained 30 min before SCT (T(0)), at 60 (T(1)) and 120 min (T(2)) after SCT. There was a threefold increase in lactate levels from baseline value at T(2) in group II, while statistically significant elevation of the lactate levels were not observed in groups III and IV. Glucose levels at T(1) and T(2) were significantly lower in groups III and IV compared with the control group. The findings of this study demonstrate that GHB can control the increase of CSF lactate and glucose levels following SCT and that this metabolic effect may be associated with neuroprotective physiological changes. 相似文献
4.
PCR based mutation screening of the laminin alpha2 chain gene (LAMA2): application to prenatal diagnosis and search for founder effects in congenital muscular dystrophy. 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Journal of medical genetics》网站下载免费的PDF全文](/ch/ext_images/free.gif)
P Guicheney N Vignier X Zhang Y He C Cruaud V Frey A Helbling-Leclerc P Richard B Estournet L Merlini H Topaloglu M Mora J P Harpey C A Haenggeli A Barois B Hainque K Schwartz F M Tomé M Fardeau K Tryggvason 《Journal of medical genetics》1998,35(3):211-217
Classical congenital muscular dystrophy with merosin deficiency is caused by mutations in the laminin alpha2 chain gene (LAMA2). Extended sequencing of the introns flanking the 64 LAMA2 exons was carried out and, based on these sequences, oligonucleotide primers were designed to amplify the coding region of each exon separately. By PCR-SSCP analysis, we identified eight new mutations in nine families originating from various countries. All induced a premature truncation of the protein, either in the short arm or in the globular C-terminal domain. A 2 bp deletion in exon 13, 2098delAG, was found in three French non-consanguineous families and a nonsense mutation of exon 20, Cys967stop, in two other non-consanguineous families originating from Italy. Determination of rare intragenic polymorphisms permitted us to show evidence of founder effects for these two mutations suggesting a remote degree of consanguinity between the families. Other, more frequent polymorphisms, G to A 1905 (exon 12), A to G 2848 (exon 19), A to G 5551 (exon 37), and G to A 6286 (exon 42), were used as intragenic markers for prenatal diagnosis. This study provides valuable methods for determining the molecular defects in LAMA2 causing merosin deficient congenital muscular dystrophy. 相似文献
5.
Bengi Balci Alp Yildiz Sezai Leventoğlu Bulent Mentes 《World journal of gastrointestinal surgery》2021,13(11):1327-1337
Retrorectal or presacral tumors are rare lesions located in the presacral area and considered as being derived from multiple embryological remnants. These tumors are classified as congenital, neurogenic, osseous, inflammatory, or miscellaneous. The most common among these are congenital benign lesions that present with non-specific symptoms, such as lower back pain and change in bowel habit. Although congenital and developmental tumors occur in younger patients, the median age of presentation is reported to be 45 years. Magnetic resonance imaging plays a crucial role in treatment management through accurate diagnosis of the lesion, the evaluation of invasion to adjacent structures, and the decision of appropriate surgical approach. The usefulness of preoperative biopsy is still debated; currently, it is only indicated for solid or heterogeneous tumors if it will alter the treatment management. Surgical resection with clear margins is considered the optimal treatment; described approaches are transabdominal, perineal, combined abdominoperineal, and minimally invasive. Benign retrorectal tumors have favorable long-term outcomes with a low incidence of recurrence, whereas malignant tumors have a potential for distant organ metastasis in addition to local recurrence. 相似文献
6.
Mohammad Obaidul Hoque Ozlem Topaloglu Shahnaz Begum Rui Henrique Eli Rosenbaum Wim Van Criekinge William H Westra David Sidransky 《Journal of clinical oncology》2005,23(27):6569-6575
PURPOSE: Aberrant promoter hypermethylation of several known or putative tumor suppressor genes occurs frequently during the pathogenesis of prostate cancers and is a promising marker for cancer detection. We sought to develop a test for prostate cancer based on a quantitative methylation-specific polymerase chain reaction (QMSP) of multiple genes in urine sediment DNA. PATIENTS AND METHODS: We tested urine sediment DNA for aberrant methylation of nine gene promoters (p16INK4a, p14(ARF), MGMT, GSTP1, RARbeta2, CDH1 [E-cadherin], TIMP3, Rassf1A, and APC) from 52 patients with prostate cancer and 21 matched primary tumors by quantitative fluorogenic real-time polymerase chain reaction. We also analyzed urine sediments from 91 age-matched individuals without any history of genitourinary malignancy as controls. RESULTS: Promoter hypermethylation of at least one of the genes studied was detected in urine samples from all 52 prostate cancer patients. Urine samples from the 91 controls without evidence of genitourinary cancer revealed no methylation of the p16, ARF, MGMT, and GSTP1 gene promoters, whereas methylation of RARbeta2, TIMP3, CDH1, Rassf1A, and APC was detected at low levels. CONCLUSION: Overall, methylation found in urine samples matched the methylation status in the primary tumor. A combination of only four genes (p16, ARF, MGMT, and GSTP1) would theoretically allow us to detect 87% of prostate cancers with 100% specificity. Our data support further development of the noninvasive QMSP assay in urine DNA for early detection and surveillance of prostate cancer. 相似文献
7.
Burak Tekin MD Mehmet Salih Gurel MD Zeynep Topkarci MD Filiz Topaloglu Demir MD Sema Aytekin MD Filiz Cebeci Kahraman MD Ralfi Singer MD Vefa Asli Erdemir MD Tugba Kevser Uzuncakmak MD Sirin Yasar MD Necmettin Akdeniz MD Ilknur Kivanc Altunay MD Emek Kocaturk MD Zafer Turkoglu MD Bilgen Erdogan MD 《Pediatric dermatology》2018,35(5):651-659
8.
9.
Clinical activity of ponatinib in one patient with chronic myeloid leukemia in chronic phase with e19a2 transcript and T315I mutation
下载免费PDF全文
![点击此处可从《European journal of haematology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
10.
Muftuoglu MA Koksal N Topaloglu U 《HPB : the official journal of the International Hepato Pancreato Biliary Association》2005,7(3):231-234
Background. Cyst hydatid disease of the liver is still endemic in certain regions of the world. Currently, surgical operation remains the treatment of choice in hydatidosis. The cyst cavity can be managed by using capitonnage, external drainage, introflexion or omentoplasty. Methods. Two hundred and thirty-five patients operated for hydatid cyst between January 1990 and February 2001 were analysed retrospectively. Either omentoplasty, external drainage, capitonnage or introflexion were used to treat residual cyst cavity. Results. Patients were categorised into three groups to evaluate complications: omentoplasty alone (group A), omentoplasty combined with other techniques (group B) and other techniques (group C). The overall mortality rates were <1%. Overall morbidity rates were 8.8% for group A, 19% for group B and 25% for group C, respectively. Mean hospital stay was 7.6 days for group A, 11.9 days for group B and 15.8 days for group C. Discussion. Postoperative surgical site infection, bile fistula, recurrence rate and overall morbidity were seen less frequently in patients who underwent omentoplasty in our series. Mean duration of hospital stay was significantly shorter in patients who underwent omentoplasty operations. Because omentum has a high absorptive capacity and the capability to fill the residual cavity, we recommend omentoplasty to manage patients with hydatid cyst of the liver, whether complicated or uncomplicated. 相似文献