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1.
2.
Localization of plasminogen activator and inhibitor, LH and androgen receptors and inhibin subunits in monkey epididymis 总被引:4,自引:1,他引:4
Epididymis is a site of sperm maturation and storage. Limited and
directed-proteolysis regulated by plasminogen activator (PA), plasminogen
activator inhibitor type-1 (PAI-1) and other related factors may play an
essential role in these processes. Our previous studies have demonstrated
that rat epididymis expressed luteinizing hormone receptor (LHR), tissue
type (t) and urokinase type (u)PA, mRNAs, and tPA activity was stimulated
in vitro by human chorionic gonoadotrophin (HCG). In the present study we
further examined localization of mRNAs for tPA, uPA, LHR, androgen receptor
(AR), as well as inhibin subunits alpha, betaA and betaB in rhesus monkey
epididymis. Using in-situ hybridization with digoxygenin-labelled cRNA
probes, we have demonstrated that tPA and PAI-1 mRNAs were localized in
epithelial cells of adult monkey epididymis. uPA mRNA was localized in the
same areas, but to a much smaller extent. tPA, uPA and PAI-1 mRNAs were
greatly expressed in the caput and corpus of adult epididymis than in other
regions. In-vitro experiments showed that both tPA and uPA activities in
epididymal cells were dramatically stimulated by HCG, but not by follicle
stimulating hormone (FSH). LHR (but not FSH receptor) and AR mRNAs were
localized in the epithelial cells of the epididymis. However, LHR mRNA was
detected in both adult and immature infant monkeys, whereas AR was found
only in the adult. Inhibin alpha, betaA and betaB mRNAs were also detected
in this organ, betaA mRNA being more strongly expressed in the caput than
in other regions of the epididymis. We suggest that LH and androgen may be
the key hormones in coordination with the PA-PAI-1 system in regulating
epididymal differentiation and sperm maturation.
相似文献
3.
Successful outcome with day 4 embryo transfer after preimplantation diagnosis for genetically transmitted diseases 总被引:4,自引:5,他引:4
Preimplantation genetic diagnosis was performed in 61 day 3 embryos
obtained by in-vitro fertilization from seven patient carriers of
haemophilia, Marfan's syndrome, Bloch-Sulzemberg syndrome (incontinentia
pigmentosa) or X chromosome-linked immune deficiency, retinitis pigmentosa,
and FG syndrome, which is characterized by mental retardation and
hypotonia. After multiplex polymerase chain reaction, 16 embryos were
diagnosed as being unaffected, and these were transferred to the uterus on
the following day (day 4). Of these embryos, six (37.5%) implanted,
resulting in the delivery of a singleton and a twin pregnancy, a late
second trimester miscarriage (twins at week 20) and a first trimester
miscarriage at week 8. All the diagnoses were confirmed by amniocentesis.
We report for the first time a late day 4 transfer of biopsied human
embryos undergoing preimplantation genetic diagnosis. This transfer
schedule allows an extra day to perform genetic analyses on single
blastomeres and to monitor any adverse effect of the biopsy procedure.
相似文献
4.
Igarashi S; Takiyama Y; Cancel G; Rogaeva EA; Sasaki H; Wakisaka A; Zhou YX; Takano H; Endo K; Sanpei K; Oyake M; Tanaka H; Stevanin G; Abbas N; Durr A; Rogaev EI; Sherrington R; Tsuda T; Ikeda M; Cassa E; Nishizawa M; Benomar A; Julien J; Weissenbach J; Tsuji S 《Human molecular genetics》1996,5(7):923-932
Machado-Joseph disease (MJD) is an autosomal dominant neurodegenerative
disorder caused by unstable expansion of a CAG repeat in the MJD1 gene at
14q32.1. To identify elements affecting the intergenerational instability
of the CAG repeat, we investigated whether the CGG/GGG polymorphism at the
3' end of the CAG repeat affects intergenerational instability of the CAG
repeat. The [expanded (CAG)n-CGG]/[normal (CAG)n- GGG] haplotypes were
found to result in significantly greater instability of the CAG repeat
compared to the [expanded (CAG)n- CGG]/[normal (CAG)n-CGG] or [expanded
(CAG)nGGG]/[normal (CAG)n-GGG] haplotypes. Multiple stepwise logistic
regression analysis revealed that the relative risk for a large
intergenerational change in the number of CAG repeat units (< -2 or >
2) is 7.7-fold (95% CI: 2.5-23.9) higher in the case of paternal
transmission than in that of maternal transmission and 7.4-fold (95% CI:
2.4-23.3) higher in the case of transmission from a parent with the
[expanded (CAG)n-CGG]/[normal (CAG)n-GGG] haplotypes than in that of
transmission from a parent with the [expanded (CAG)n-CGG]/[normal
(CAG)n-CGG] or [expanded (CAG)n- GGG]/[normal (CAG)n-GGG] haplotypes. The
combination of paternal transmission and the [expanded (CAG)n-CGG]/[normal
(CAG)n-GGG] haplotypes resulted in a 75.2-fold (95% CI: 9.0-625.0) increase
in the relative risk compared with that of maternal transmission and the
[expanded (CAG)n-CGG]/[normal (CAG)n-CGG] or [expanded (CAG)n- GGG]/[normal
(CAG)n-GGG] haplotypes. The results suggest that an inter- allelic
interaction is involved in the intergenerational instability of the
expanded CAG repeat.
相似文献
5.
1 病例报告 男 ,16岁 .误食毒蕈后出现恶心 ,呕吐伴有腹痛、腹泻 4d,每日大便 4~ 12次 ,为稀水便 ,有心慌、气短、发热 ,在当地医院以“毒蕈中毒”救治 ,症状无缓解 ,为进一步治疗于 2 0 0 0 - 0 8- 31转入我院 .查体 :T37.8℃ ,P110次·min-1 ,R18次·min-1 ,BP14/ 9k Pa.全身皮肤黄染 ,巩膜重度黄染 ,心肺无阳性体征 .腹平坦 ,上腹部有压痛 ,肌紧张及反跳痛 ,肝脾未触及 ,肝区有叩击痛 ,移动性浊音阳性 .入院后患者出现烦躁 ,意识不清 ,心率时快时慢 ,血压不稳定 .肝功 :T- BIL 2 5 4.1μmol· L-1 ,D- BIL 16 9.5 μmol· L-1 … 相似文献
6.
下消化道出血221例分析 总被引:4,自引:2,他引:2
0 引言 下消化道出血是消化科的常见病 ,是指十二指肠空肠移行部 ,屈氏韧带以下的小肠和大肠疾病引起的肠道出血 .有人认为一般不包括痔和肛裂出血 [1 ] .临床最常见为慢性出血 ,但有时出血量大危及生命 ,需要做紧急处理 ,现将我院1992 - 0 1/ 1999- 0 8门诊及病房收治的 2 2 1例下消化道出血进行分析 ,报告如下 :1 临床资料 男 12 0例 ,女 10 1例 ,年龄 14~ 72 (平均 42 )岁 ,病程 16 h~ 10 a.患者分别以脓血便、暗红色血便、鲜血便或果酱色血便为主诉前来就诊 ,其中有休克症状的大出血者6例 .血 Hb<110 g·L- 1 40例 ,<80 g·L- 1… 相似文献
7.
0 引言 我院 1996 - 0 6 / 1998- 0 7用奥曲肽治疗肝硬变食管静脉曲张破裂出血 19例 ,并与垂体后叶素作了对照 .1 对象和方法1.1 对象 经内镜证实食管静脉曲张破裂出血 37例 ;男 32例 ,女 5例 ;年龄 42~ 6 9岁 .肝炎后肝硬变 32例 ,乙醇性肝硬变 3例 ,血吸虫性肝硬变 2例 .按就诊顺序随机分为治疗组19例 ,对照 18例 .估计出血量 :治疗组 (146 3.6± 375 .8) m L;对照组 (144 9.5± 36 8.4) m L .出血至治疗间隔时间 :治疗组(11.6± 3.5 ) h;对照组 (11.2± 3.6 ) h.两组的年龄、性别、肝功 Child分级、食管静脉曲张程度、出血量、出… 相似文献
8.
五灵胶囊治疗慢性肝炎的临床随访观察 总被引:13,自引:3,他引:10
0 引言 五灵丸治疗慢性病毒性肝炎取得了较好的疗效 [1 ] ,但五灵丸为大蜜丸 ,不易被患者所接受 ,且其消化道副作用较明显 .为此 ,我们改进剂型 ,研制成五灵胶囊 ,为明确其能否保持远期疗效 ,我们对用该药治疗的患者进行随访观察 ,同时与五灵丸的疗效进行比较 .1 对象和方法1.1 病例选择 按慢性肝炎的诊断标准选择住院患者 10 0例 ,随机分为两组 .治疗组为五灵胶囊组 ,对照组为五灵丸组 .每组各 5 0例 .治疗组轻度 19例 ,中度 2 0例 ,重度 11例 .对照组轻度 2 0例 ,中度 18例 ,重度 12例 .两组均选择 18~5 8岁 ,病程 1~ 2 0 a.平均 4… 相似文献
9.
1 病例报告 女 ,45岁 ,因吞咽困难 16 mo伴发热 15 d,于1999- 0 7- 12入院 .于 16 mo前与人争吵后出现吞咽困难 ,以进食馒头、面条为著 ,剑突下有时出现梗噎感 ,随即呕吐 ,呕出所进食物及粘液 ,情绪波动可诱发症状加重 ,伴食欲减退、返酸 .15 d前无诱因出现不规则发热 ,T38~ 39℃ ,上腹痛加重 ,全身酸困不适 ,乏力、头晕、消瘦 .追问病史 ,患者 5 a前双手、足疼痛麻木 ,尤以受凉、精神刺激后明显 ,手指初发白 ,继而发紫 ,变红 ,且麻木疼痛加重 ,手足小关节渐僵硬、畸形 .曾到多家医院就诊 ,诊断 :“雷诺病、类风湿性关节炎”.2 a前出现… 相似文献
10.