前列腺癌癌基因表达和DNA倍体分析

８８例前列腺癌组织应用免疫组化、图像分析及流式细胞仪（ＦＣＭ）方法，检测癌基因Ｐ~（５３），ｃ－ｅｒｂＢ－２及增殖细胞核抗原ＰＣＮＡ的表达及ＤＮＡ含量。Ｐ~（５３）异常表达率为２８％（２５／８８），ｃ－ｅｒｂＢ－２过度表达率为６８％（６０／８８）．ＰＣＮＡ（＞５０％）增殖指数值为７４％（６５／８８）．与对照组前列腺良性增生及正常前列腺组织中呈阴性反应对比有明显差异（Ｐ＜０．０５），与癌组织分化程度、临床分期及ＤＮＡ含量无明显相关（Ｐ＞０．０５）。７７例癌旁前列腺基底细胞异型增生上皮中有９例（１２％）亦显示Ｐ~（５３），ｃ－ｅｒｂＢ－２及ＰＣＮＡ的异常表达，提示该异型增生的基底细胞具有恶性表型。表明前列腺癌中Ｐ~（５３），ｃ－ｅｒｂＢ－２及ＰＣＮＡ的异常表达与癌发生有一定关系，以ｃ－ｅｒｂＢ－２过度表达尤为重要，３种抗体的联合应用可作为前列腺癌的肿瘤标志物，对前列腺癌发病机理研究及预测早癌发生有一定价值，但作为患者预后判断的指标尚显不足。     

异叶青兰作用后的缺氧大鼠海马超微结构的定量分析

本文采用图像定量分析和形态测量方法,对缺氧及服用中草药异叶青兰的大鼠海马CA3区超微结构进行形态定量观察.结果表明,缺氧组海马锥体细胞肿胀;粗面内质网脱颗粒、网池扩张、断裂,并且失去正常的绕核或平行成层的分布形式;线粒体明显肿胀、嵴断裂,数量减少,平均截面积增大;髓鞘变性,轴突和树突内线粒体肿胀明显;轴棘、轴树突触间隙宽度变窄.缺氧给药组海马锥体细胞无明显肿胀;线粒体数量增多,平均截面积无明显增大,线粒体嵴结构完整.本文对各组形态变化的差异进行了探讨.     

Cloning of a novel, anonymous gene from a megabase-range YAC and cosmid contig in the neurofibromatosis type 2/meningioma region on human chromosome 22q12

In order to permit detailed characterization of meningioma casesshowing deletions within chromosomal band 22q12 and furthersystematically clone genes located within this region, we establisheda genomic YAC and cosmid contig which encompasses a region inexcess of 1000 kb of 22q12. The YAC contig consists of 6 YACclones arranged Into 5 overlapping steps covering more than1100 kb. Two corresponding cosmid contigs consisting of 40 stepsof overlapping groups of cosmids encompasses 900–1000kb. This set of genomic clones provides a detailed physicalmap of this part of chromosome 22 and constitutes a basis forthe Isolation and characterization of genes that may be locatedwithin this chromosomal region. Employing the exon-amplificationmethod on two cosmids from the contig, we cloned a novel, anonymousgene, pK1.3, which potentially encodes a protein of 683 aminoacids with a predicted molecular weight of of 78.5 kD. Its 2.7kb mRNA is expressed ubiquitously. We estimated the genomicsize of this gene to 100 –150 kb, and it is located inthe Immediate centromeric vicinity of the neurofibromatosis2 (NF2) tumor suppressor gene.