全文获取类型
收费全文 | 934篇 |
免费 | 351篇 |
国内免费 | 12篇 |
专业分类
耳鼻咽喉 | 4篇 |
儿科学 | 25篇 |
妇产科学 | 92篇 |
基础医学 | 30篇 |
口腔科学 | 5篇 |
临床医学 | 710篇 |
内科学 | 118篇 |
皮肤病学 | 11篇 |
神经病学 | 62篇 |
特种医学 | 42篇 |
外科学 | 60篇 |
综合类 | 21篇 |
预防医学 | 66篇 |
眼科学 | 1篇 |
药学 | 17篇 |
中国医学 | 1篇 |
肿瘤学 | 32篇 |
出版年
2023年 | 3篇 |
2022年 | 1篇 |
2021年 | 5篇 |
2020年 | 6篇 |
2019年 | 9篇 |
2018年 | 70篇 |
2017年 | 94篇 |
2016年 | 111篇 |
2015年 | 99篇 |
2014年 | 86篇 |
2013年 | 113篇 |
2012年 | 48篇 |
2011年 | 48篇 |
2010年 | 66篇 |
2009年 | 87篇 |
2008年 | 65篇 |
2007年 | 51篇 |
2006年 | 39篇 |
2005年 | 33篇 |
2004年 | 40篇 |
2003年 | 17篇 |
2002年 | 13篇 |
2001年 | 11篇 |
2000年 | 9篇 |
1999年 | 8篇 |
1998年 | 14篇 |
1997年 | 20篇 |
1996年 | 17篇 |
1995年 | 9篇 |
1994年 | 8篇 |
1993年 | 8篇 |
1992年 | 5篇 |
1991年 | 2篇 |
1990年 | 6篇 |
1989年 | 10篇 |
1988年 | 8篇 |
1987年 | 10篇 |
1986年 | 4篇 |
1985年 | 11篇 |
1984年 | 10篇 |
1983年 | 4篇 |
1982年 | 3篇 |
1981年 | 4篇 |
1980年 | 2篇 |
1979年 | 5篇 |
1978年 | 2篇 |
1977年 | 2篇 |
1975年 | 1篇 |
排序方式: 共有1297条查询结果,搜索用时 15 毫秒
1.
2.
PADGEM (GMP140) is a component of Weibel-Palade bodies of human endothelial cells 总被引:65,自引:10,他引:55
PADGEM protein (PADGEM), also known as GMP140, is a platelet alpha- granule membrane protein that is translocated to the external membrane after platelet activation. Although the biosynthesis of this protein was originally thought to be confined to megakaryocytes, the synthesis of PADGEM in endothelial cells was recently demonstrated (McEver et al: Blood 70:1974a, 1987). We now describe the subcellular localization of this protein in endothelial cells. Immunofluorescence staining of permeabilized human umbilical vein endothelial cells with KC4, a well characterized monoclonal antibody to PADGEM, showed positively stained elongated structures similar in distribution and shape to Weibel-Palade bodies. Their identity as Weibel-Palade bodies was confirmed by double label immunofluorescence using KC4 and a polyclonal antiserum to von Willebrand factor (vWf), a protein known to be specifically stored in these organelles. All Weibel-Palade bodies were found to contain PADGEM. In contrast to strong perinuclear staining produced with anti- vWf antibodies, no significant perinuclear staining was obtained with KC4, indicating that relatively little PADGEM is present in the endoplasmic reticulum and in the Golgi apparatus. In endothelial cells treated with secretagogues that stimulate vWf release the elongated structures positive for PADGEM disappeared, further identifying these structures as Weibel-Palade bodies. This observation extends the parallels between Weibel-Palade bodies and alpha-granules and suggests a possible functional association between vWf and PADGEM. 相似文献
3.
This column contains the presidential address presented during the Third Annual Meeting of the American Association of Heart Failure Nurses on June 28, 2007, in San Diego, California, titled "Building the Foundation of Excellence in Heart Failure Nursing." 相似文献
4.
5.
6.
7.
8.
M. Ono C. G. Winearls N. Amos D. Grennan A. Gharavi D. K. Peters J. G. P. Sissons 《European journal of immunology》1987,17(3):343-349
Four human monoclonal rheumatoid factors (MRF) were used to raise a panel of mouse monoclonal antibodies (mAb) which were selected in a solid-phase radioimmunoassay for binding to MRF but not normal IgM. Three mAb, each raised against a different MRF, bound to the majority of MRF and also to most polyclonal RF. Four other mAb bound selectively to the MRF against which they were raised and to no other MRF, and rarely to any polyclonal RF. Competition studies using cold and radiolabeled mAb further indicated that these mAb recognize distinct and different epitopes on MRF. RF activity of MRF was inhibited by 3 of the 4 mAb binding to a single MRF and 2 of the 3 mAb binding to multiple RF. It was thus concluded that of this panel of mAb 3 recognized cross-reactive idiotopes and the remainder demonstrated highly restricted idiotopes on MRF. These mAb identified MRF idiotopebearing cells in the peripheral blood of 3 of the MRF donors (and a further subject with type II essential cryoglobulinemia), with a frequency ranging from 0.3–10% of all mononuclear cells with the mAb to restricted idiotopes or 1.5–17% with mAb to cross-reactive idiotopes. These anti-idiotopic mAb should thus provide a highly specific means of identifying and monitoring MRF-producing cells in vivo. 相似文献
9.
10.
Did you ever think, “If we just had a little money we could…”? The current health care environment is wrought with financial stressors that can be overwhelming and take up most of our time. Such stress can limit the development of a professional practice environment if you let it. How do you not only survive but thrive in this financial climate? 相似文献