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1.
Immunohistochemical study of proliferating cell nuclear antigen (PCNA) in duckling liver fed with aflatoxin B1 and esterified glucomannan 总被引:1,自引:0,他引:1
The effect of esterified glucomannan on aflatoxin B1 toxicity in ducklings was studied by immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in hepatic cells on formalin-fixed paraffin-embedded liver samples. Cherry Valley ducklings were divided into five groups, 20 birds in each. One of the groups was fed with conventional feed, and the other groups were fed with diet containing 100 ppb aflatoxin B1, that containing 0.05% esterified glucomannan, or that containing 100 ppb aflatoxin B1 supplemented with 0.05 or 0.1% esterified glucomannan, from five days of age for one month, and subsequently all the groups were fed with conventional feed for 20 days. Four birds of each group were sacrificed on the 30th, 35th, 40th, 45th and 50th day of feeding, and PCNA on the liver tissue sections was quantitatively analyzed by immunohistochemical staining. The percentage of PCNA-positive hepatocytes was significantly higher in the group given diet containing aflatoxin B1 than in the other groups, which were not significantly different from each other. The results demonstrate that supplementation of feed with esterified glucomannan is effective in reduction of aflatoxin B1-induced hepatic injury in ducklings. 相似文献
2.
T. Kasantikul A. Sommanustweechai K. Polsrila W. Kongkham C. Chaisongkram S. Sanannu P. Kongmakee W. Narongwanichgarn M. Bush R. W. Sermswan W. Banlunara 《Transboundary and Emerging Diseases》2016,63(5):e389-e394
Melioidosis is caused by Burkholderia pseudomallei and is an important zoonotic infectious disease causing high mortality from fulminant septicaemia in humans and a wide variety of animal species. The incidence of fatal melioidosis in zoo animals has been significant in many Thai zoos. A total number of 32 cases were evaluated throughout the Thai zoo animal populations. The highest prevalence of disease has been reported from the north‐eastern region followed by the zoos in the southern part of the country, approximately 47% and 38%, respectively, while the other zoos reported sporadic infections. Herbivores and non‐human primates were the most commonly affected animals with incidences of 59% and 28%, respectively. This appears to be a seasonal correlation with the highest incidence of melioidosis in zoo animals reported in the rainy season (44%) or subdivided monthly in June (19%) followed by September and November (16% and 12%, respectively). The route of infection and the incubation period still remain unclear. This retrospective study examined the clinical presentation in various zoo species, pathological findings and epidemiological data as well as conducting an in depth literature review. 相似文献
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Suwimon Jettanacheawchankit Siriruk Sasithanasate Polkit Sangvanich Wijit Banlunara Pasutha Thunyakitpisal 《Journal of pharmacological sciences》2009,109(4):525-531
Aloe vera has long been used as a traditional medicine for inducing wound healing. Gingival fibroblasts (GFs) play an important role in oral wound healing. In this study, we investigated the effects of acemannan, a polysaccharide extracted from Aloe vera gel, on GF proliferation; keratinocyte growth factor-1 (KGF-1), vascular endothelial growth factor (VEGF), and type I collagen production; and oral wound healing in rats. [3H]-Thymidine incorporation assay and ELISA were used. Punch biopsy wounds were created at the hard palate of male Sprague Dawley rats. All treatments (normal saline; 0.1% triamcinolone acetonide; plain 1% Carbopol®; and Carbopol® containing 0.5%, 1%, and 2% acemannan (w/w)) were applied daily. Wounded areas and histological features were observed at day 7 after treatment. From our studies, acemannan at concentrations of 2, 4, 8, and 16 mg/ml significantly induced cell proliferation (P<0.05). Acemannan concentrations between 2 – 16 mg/ml significantly stimulated KGF-1, VEGF, and type I collagen expressions (P<0.05). Wound healing of animals receiving Carbopol® containing 0.5% acemannan (w/w) was significantly better than that of the other groups (P<0.05). These findings suggest that acemannan plays a significant role in the oral wound healing process via the induction of fibroblast proliferation and stimulation of KGF-1, VEGF, and type I collagen expressions. 相似文献
5.
Sianglum W Wonglumsom W Srimanote P Kittiniyom K 《The Southeast Asian journal of tropical medicine and public health》2007,38(6):1095-1101
Escherichia coli was used to investigate quinolone resistance and mutations in gyrA gene of E. coli isolated from pet (dog and cat), human (pet's owner), vegetable and edible ice in Bangkok and vicinity. Susceptibility test for nalidixic acid (NA) showed similar percent resistance among the sample sources but a lower ciprofloxacin (CIP) resistance was found particularly in human source. Mutations within quinolone resistance determining region of gyrA gene analyzed using non-radioactive single-strand conformation polymorphism (SSCP) and sequencing showed 10 different SSCP patterns. E. coli isolates from pet, vegetable and ice showed more variety of patterns than strains isolated from human. Four out of 10 SSCP patterns were identified as having mutations in amino acids positions 83 (Ser to Leu) and position 87 (Asp to Asn). These mutations were observed only in NA-resistant strains and combined mutations were observed only in E. coli isolated from humans and pets. As only 24% of NA- and CIP-resistant E coli isolates contained gyrA mutations, other quinolone resistant mechanisms may be involved. Nevertheless, gyrA mutations may be used to monitor nalidixid acid resistance in E. coli. 相似文献
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Thitinun Monhaphol Sirinthorn Yibchok-anun Wijit Banlunara Mayura Wittayasuporn Tanapat Palaga Pravit Asawanonda 《Drug and chemical toxicology》2013,36(2):289-301
Safety of two new ultraviolet (UV) filters, 2-ethylhexyl-2,4,5-trimethoxycinnamate (E8) and 2-ethylhexyl-2,4,5-trimethoxybenzalmalonate (B8), has been evaluated through the human melanoma cytotoxicity test and seven-day acute oral toxicity studies in rats. At 2.5 mg/mL, both compounds gave similar cell viability to the control. LD50 values for E8 and B8 are more than 5000 and 1000 mg/kg body weight, respectively. No significant difference in body weight and hematological parameters among the 0, 5, 50, 500, and 5000 mg/Kg E8-treated animals could be detected. Pathological examination of rat tissues collected at the end of the study period revealed no significant difference between the control and all E8-administered rats. There was no significant difference in all clinical blood chemistry parameters (aspartate aminotransferase, creatinine, blood urea nitrogen, and cholesterol), except alanine aminotransferase (ALT), between the control and the E8-treated animals. All ALT values were, however, in the normal range of SD rats. E8 showed negative results for the skin irritation study on human volunteers, using patch and photopatch tests. Excitation of respiratory signs of dypsnea in 10, 100, and 1000 mg/Kg B8-treated rats could be observed during 1–24 h. All groups were, however, normal during the second to the seventh day. Hematological parameters of the 0, 10, 100, and 1000 mg/Kg B8-treated animals showed no significant difference. Pathological examination revealed no significant difference between the control and all B8-administered rats. However, significant differences in some clinical blood chemistry parameters and body weights between the control and some B8-treated animals could be detected. All values, however, were in the normal ranges of the SD rats. 相似文献
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Monhaphol T Yibchok-Anun S Banlunara W Wittayasuporn M Palaga T Asawanonda P Wanichweacharungruang S 《Drug and chemical toxicology》2008,31(2):289-301
Safety of two new ultraviolet (UV) filters, 2-ethylhexyl-2,4,5-trimethoxycinnamate (E8) and 2-ethylhexyl-2,4,5-trimethoxybenzalmalonate (B8), has been evaluated through the human melanoma cytotoxicity test and seven-day acute oral toxicity studies in rats. At 2.5 mg/mL, both compounds gave similar cell viability to the control. LD50 values for E8 and B8 are more than 5000 and 1000 mg/kg body weight, respectively. No significant difference in body weight and hematological parameters among the 0, 5, 50, 500, and 5000 mg/Kg E8-treated animals could be detected. Pathological examination of rat tissues collected at the end of the study period revealed no significant difference between the control and all E8-administered rats. There was no significant difference in all clinical blood chemistry parameters (aspartate aminotransferase, creatinine, blood urea nitrogen, and cholesterol), except alanine aminotransferase (ALT), between the control and the E8-treated animals. All ALT values were, however, in the normal range of SD rats. E8 showed negative results for the skin irritation study on human volunteers, using patch and photopatch tests. Excitation of respiratory signs of dypsnea in 10, 100, and 1000 mg/Kg B8-treated rats could be observed during 1-24 h. All groups were, however, normal during the second to the seventh day. Hematological parameters of the 0, 10, 100, and 1000 mg/Kg B8-treated animals showed no significant difference. Pathological examination revealed no significant difference between the control and all B8-administered rats. However, significant differences in some clinical blood chemistry parameters and body weights between the control and some B8-treated animals could be detected. All values, however, were in the normal ranges of the SD rats. 相似文献
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Natthakitta SuwannateepWijit Banlunara Supason P. Wanichwecharungruang Khajeelak Chiablaem Kriengsak LirdprapamongkolJisnuson Svasti 《Journal of controlled release》2011,151(2):176-182
Although mucoadhesive drug carriers for the gastro-intestinal tract (GIT) have been reported, the mucoadhesive property and drug release characteristics have never been evaluated separately, whilst the adherence of the carriers to the surface of GIT has not been directly visualized. Here, a monopolymeric carrier made from ethylcellulose (EC) and a dipolymeric carrier made from a blend of methylcellulose (MC) and EC (ECMC) were easily fabricated through a self-assembling process and yielded the highest reported curcumin loading of ~ 48-49%. Both curcumin loaded ECMC (C-ECMC) and curcumin loaded EC (C-EC) particles showed an in vitro free radical scavenging activity and a dose-dependent in vitro cytotoxic effect towards MCF-7 human breast adenocarcinoma and HepG2 hepatoblastoma cells in tissue culture. The in vivo evaluation of their adherence to stomach mucosa and their ability to release curcumin into the circulation were carried out through quantification of curcumin levels in the stomach tissue and in blood of mice orally administered with the two spheres. Direct evidence of the adherence of the C-EC and C-ECMC particles along the mucosal epithelia of the stomach is also presented for the first time through SEM images. The mucoadhesive property of self-assembled C-EC nanoparticles is discussed. 相似文献
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A male 2.5-year-old American short hair cat was admitted to the animal hospital with the primary complaints of abdominal distension and depression for several days. Ultrasound revealed a large hypoechoic cavity with a well demarcated thin wall on a liver lobe. On exploratory laparotomy, a hepatic haematoma was observed. Subsequently, hepatic lobectomy was performed. Histologically, an eosinophilic substance stained with Congo red was deposited in the cyst wall which showed an optical property of apple green birefringence under polarized light. Based on immunohistochemical staining, amyloid A was diagnosed. To our knowledge, this is the first report of a spontaneous hepatic haematoma with an amyloid A deposition in a cat. 相似文献
10.
Nabhat Thongsoi Nataya Charoenvisal Wijit Banlunara Nopadon Pirarat Anudep Rungsipipat 《Comparative clinical pathology》2012,21(6):1753-1755
Our purpose is to report a rare case of lipoid pneumonia found in a cat with diabetes mellitus (DM) type II. The cat is an aged, mixed-breed male with a 3-year history of having DM. During the last 16?weeks of its life, the cat presented signs of dyspnea and weakness and was continuously treated by nebulization. Thoracic radiography showed an increased radiopacity of the alveoli. The histopathological findings showed typical lipoid pneumonia, proliferative bronchitis, and diffuse suppurative pneumonia. Chronic pancreatitis with islet amyloidosis and arteriosclerosis was also detected. Immunohistochemistry with anti-Amylin antibody showed a strong positive result in the islet of Langerhans, confirming type II DM in this case. A diagnosis of death from respiratory failure associated with idiopathic lipoid pneumonia was achieved. 相似文献