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1.
Immunohistochemical study of proliferating cell nuclear antigen (PCNA) in duckling liver fed with aflatoxin B1 and esterified glucomannan 总被引:1,自引:0,他引:1
The effect of esterified glucomannan on aflatoxin B1 toxicity in ducklings was studied by immunohistochemical staining of proliferating cell nuclear antigen (PCNA) in hepatic cells on formalin-fixed paraffin-embedded liver samples. Cherry Valley ducklings were divided into five groups, 20 birds in each. One of the groups was fed with conventional feed, and the other groups were fed with diet containing 100 ppb aflatoxin B1, that containing 0.05% esterified glucomannan, or that containing 100 ppb aflatoxin B1 supplemented with 0.05 or 0.1% esterified glucomannan, from five days of age for one month, and subsequently all the groups were fed with conventional feed for 20 days. Four birds of each group were sacrificed on the 30th, 35th, 40th, 45th and 50th day of feeding, and PCNA on the liver tissue sections was quantitatively analyzed by immunohistochemical staining. The percentage of PCNA-positive hepatocytes was significantly higher in the group given diet containing aflatoxin B1 than in the other groups, which were not significantly different from each other. The results demonstrate that supplementation of feed with esterified glucomannan is effective in reduction of aflatoxin B1-induced hepatic injury in ducklings. 相似文献
2.
Suwimon Jettanacheawchankit Siriruk Sasithanasate Polkit Sangvanich Wijit Banlunara Pasutha Thunyakitpisal 《Journal of pharmacological sciences》2009,109(4):525-531
Aloe vera has long been used as a traditional medicine for inducing wound healing. Gingival fibroblasts (GFs) play an important role in oral wound healing. In this study, we investigated the effects of acemannan, a polysaccharide extracted from Aloe vera gel, on GF proliferation; keratinocyte growth factor-1 (KGF-1), vascular endothelial growth factor (VEGF), and type I collagen production; and oral wound healing in rats. [3H]-Thymidine incorporation assay and ELISA were used. Punch biopsy wounds were created at the hard palate of male Sprague Dawley rats. All treatments (normal saline; 0.1% triamcinolone acetonide; plain 1% Carbopol®; and Carbopol® containing 0.5%, 1%, and 2% acemannan (w/w)) were applied daily. Wounded areas and histological features were observed at day 7 after treatment. From our studies, acemannan at concentrations of 2, 4, 8, and 16 mg/ml significantly induced cell proliferation (P<0.05). Acemannan concentrations between 2 – 16 mg/ml significantly stimulated KGF-1, VEGF, and type I collagen expressions (P<0.05). Wound healing of animals receiving Carbopol® containing 0.5% acemannan (w/w) was significantly better than that of the other groups (P<0.05). These findings suggest that acemannan plays a significant role in the oral wound healing process via the induction of fibroblast proliferation and stimulation of KGF-1, VEGF, and type I collagen expressions. 相似文献
3.
Sianglum W Wonglumsom W Srimanote P Kittiniyom K 《The Southeast Asian journal of tropical medicine and public health》2007,38(6):1095-1101
Escherichia coli was used to investigate quinolone resistance and mutations in gyrA gene of E. coli isolated from pet (dog and cat), human (pet's owner), vegetable and edible ice in Bangkok and vicinity. Susceptibility test for nalidixic acid (NA) showed similar percent resistance among the sample sources but a lower ciprofloxacin (CIP) resistance was found particularly in human source. Mutations within quinolone resistance determining region of gyrA gene analyzed using non-radioactive single-strand conformation polymorphism (SSCP) and sequencing showed 10 different SSCP patterns. E. coli isolates from pet, vegetable and ice showed more variety of patterns than strains isolated from human. Four out of 10 SSCP patterns were identified as having mutations in amino acids positions 83 (Ser to Leu) and position 87 (Asp to Asn). These mutations were observed only in NA-resistant strains and combined mutations were observed only in E. coli isolated from humans and pets. As only 24% of NA- and CIP-resistant E coli isolates contained gyrA mutations, other quinolone resistant mechanisms may be involved. Nevertheless, gyrA mutations may be used to monitor nalidixid acid resistance in E. coli. 相似文献
4.
Thitinun Monhaphol Sirinthorn Yibchok-anun Wijit Banlunara Mayura Wittayasuporn Tanapat Palaga Pravit Asawanonda 《Drug and chemical toxicology》2013,36(2):289-301
Safety of two new ultraviolet (UV) filters, 2-ethylhexyl-2,4,5-trimethoxycinnamate (E8) and 2-ethylhexyl-2,4,5-trimethoxybenzalmalonate (B8), has been evaluated through the human melanoma cytotoxicity test and seven-day acute oral toxicity studies in rats. At 2.5 mg/mL, both compounds gave similar cell viability to the control. LD50 values for E8 and B8 are more than 5000 and 1000 mg/kg body weight, respectively. No significant difference in body weight and hematological parameters among the 0, 5, 50, 500, and 5000 mg/Kg E8-treated animals could be detected. Pathological examination of rat tissues collected at the end of the study period revealed no significant difference between the control and all E8-administered rats. There was no significant difference in all clinical blood chemistry parameters (aspartate aminotransferase, creatinine, blood urea nitrogen, and cholesterol), except alanine aminotransferase (ALT), between the control and the E8-treated animals. All ALT values were, however, in the normal range of SD rats. E8 showed negative results for the skin irritation study on human volunteers, using patch and photopatch tests. Excitation of respiratory signs of dypsnea in 10, 100, and 1000 mg/Kg B8-treated rats could be observed during 1–24 h. All groups were, however, normal during the second to the seventh day. Hematological parameters of the 0, 10, 100, and 1000 mg/Kg B8-treated animals showed no significant difference. Pathological examination revealed no significant difference between the control and all B8-administered rats. However, significant differences in some clinical blood chemistry parameters and body weights between the control and some B8-treated animals could be detected. All values, however, were in the normal ranges of the SD rats. 相似文献
5.
Monhaphol T Yibchok-Anun S Banlunara W Wittayasuporn M Palaga T Asawanonda P Wanichweacharungruang S 《Drug and chemical toxicology》2008,31(2):289-301
Safety of two new ultraviolet (UV) filters, 2-ethylhexyl-2,4,5-trimethoxycinnamate (E8) and 2-ethylhexyl-2,4,5-trimethoxybenzalmalonate (B8), has been evaluated through the human melanoma cytotoxicity test and seven-day acute oral toxicity studies in rats. At 2.5 mg/mL, both compounds gave similar cell viability to the control. LD50 values for E8 and B8 are more than 5000 and 1000 mg/kg body weight, respectively. No significant difference in body weight and hematological parameters among the 0, 5, 50, 500, and 5000 mg/Kg E8-treated animals could be detected. Pathological examination of rat tissues collected at the end of the study period revealed no significant difference between the control and all E8-administered rats. There was no significant difference in all clinical blood chemistry parameters (aspartate aminotransferase, creatinine, blood urea nitrogen, and cholesterol), except alanine aminotransferase (ALT), between the control and the E8-treated animals. All ALT values were, however, in the normal range of SD rats. E8 showed negative results for the skin irritation study on human volunteers, using patch and photopatch tests. Excitation of respiratory signs of dypsnea in 10, 100, and 1000 mg/Kg B8-treated rats could be observed during 1-24 h. All groups were, however, normal during the second to the seventh day. Hematological parameters of the 0, 10, 100, and 1000 mg/Kg B8-treated animals showed no significant difference. Pathological examination revealed no significant difference between the control and all B8-administered rats. However, significant differences in some clinical blood chemistry parameters and body weights between the control and some B8-treated animals could be detected. All values, however, were in the normal ranges of the SD rats. 相似文献
6.
Nabhat Thongsoi Nataya Charoenvisal Wijit Banlunara Nopadon Pirarat Anudep Rungsipipat 《Comparative clinical pathology》2012,21(6):1753-1755
Our purpose is to report a rare case of lipoid pneumonia found in a cat with diabetes mellitus (DM) type II. The cat is an aged, mixed-breed male with a 3-year history of having DM. During the last 16?weeks of its life, the cat presented signs of dyspnea and weakness and was continuously treated by nebulization. Thoracic radiography showed an increased radiopacity of the alveoli. The histopathological findings showed typical lipoid pneumonia, proliferative bronchitis, and diffuse suppurative pneumonia. Chronic pancreatitis with islet amyloidosis and arteriosclerosis was also detected. Immunohistochemistry with anti-Amylin antibody showed a strong positive result in the islet of Langerhans, confirming type II DM in this case. A diagnosis of death from respiratory failure associated with idiopathic lipoid pneumonia was achieved. 相似文献
7.
A male 2.5-year-old American short hair cat was admitted to the animal hospital with the primary complaints of abdominal distension and depression for several days. Ultrasound revealed a large hypoechoic cavity with a well demarcated thin wall on a liver lobe. On exploratory laparotomy, a hepatic haematoma was observed. Subsequently, hepatic lobectomy was performed. Histologically, an eosinophilic substance stained with Congo red was deposited in the cyst wall which showed an optical property of apple green birefringence under polarized light. Based on immunohistochemical staining, amyloid A was diagnosed. To our knowledge, this is the first report of a spontaneous hepatic haematoma with an amyloid A deposition in a cat. 相似文献
8.
Sadaow Lakkhana Sanpool Oranuch Phosuk Issarapong Rodpai Rutchanee Thanchomnang Tongjit Wijit Adulsak Anamnart Witthaya Laymanivong Sakhone Aung Win Pa Pa Janwan Penchom Maleewong Wanchai Intapan Pewpan M. 《Parasitology research》2018,117(8):2427-2436
Parasitology Research - Ascaris lumbricoides is the largest roundworm known from the human intestine while Ascaris suum is an internal parasite of pigs. Ascariasis, caused by Ascaris lumbricoides,... 相似文献
9.
Sani Boonyagul Wijit Banlunara Polkit Sangvanich Pasutha Thunyakitpisal 《Odontology / the Society of the Nippon Dental University》2014,102(2):310-317
Aloe vera is a traditional wound healing medicine. We hypothesized acemannan, a polysaccharide extracted from Aloe vera gel, could affect bone formation. Primary rat bone marrow stromal cells (BMSCs) were treated with various concentrations of acemannan. New DNA synthesis, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein, osteopontin expression, and mineralization were determined by [3H] thymidine incorporation assay, ELISA, biochemical assay, western blotting, and Alizarin Red staining, respectively. In an animal study, mandibular right incisors of male Sprague–Dawley rats were extracted and an acemannan treated sponge was placed in the socket. After 1, 2, and 4 weeks, the mandibles were dissected. Bone formation was evaluated by dual-energy X-ray absorptiometry and histopathological examination. The in vitro results revealed acemannan significantly increased BMSC proliferation, VEGF, BMP-2, alkaline phosphatase activity, bone sialoprotein and osteopontin expression, and mineralization. In-vivo results showed acemannan-treated groups had higher bone mineral density and faster bone healing compared with untreated controls. A substantial ingrowth of bone trabeculae was observed in acemannan-treated groups. These data suggest acemannan could function as a bioactive molecule inducing bone formation by stimulating BMSCs proliferation, differentiation into osteoblasts, and extracellular matrix synthesis. Acemannan could be a candidate natural biomaterial for bone regeneration. 相似文献
10.
Wijit Kiatipattanasakul Shin-ichiro Nakamura Mokbul M. Hossain Hiroyuki Nakayama Tomiya Uchino Seigou Shumiya Naoaki Goto K. Doi 《Acta neuropathologica》1996,92(3):242-248
Apoptosis similar to that seen in Alzheimer’s disease patients was found in the brain of aged dogs by the TUNEL method of
detecting in situ DNA fragmentation. Apoptosis was observed in both neurons and glial cells, and was morphologically characterized
by round and swollen cytoplasm and aggregated nuclear chromatin, although these changes were slight. Neurons and astrocytes
in the gray matter and oligodendrocytes in the white matter were affected. The number of ApopTag-positive brain cells increased
slightly with age, but was not correlated to the number of senile plaques. A good correlation between the number of ApopTag-positive
cells and the dementia index was clearly found. The present study indicates that brain cell apoptosis could account for dementia
in aged dogs and suggested that aged dogs may be useful as a simplified animal model for Alzheimer’s disease in man.
Received: 25 January 1996 / Revised, accepted: 15 March 1996 相似文献