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排序方式: 共有329条查询结果,搜索用时 31 毫秒
1.
Kazunori Aizawa Takeshi Hanaoka Hiroki Kasai Kaoru Kogashi Setsuo Kumazaki Jun Koyama Hiroshi Tsutsui Yoshikazu Yazaki Noboru Watanabe Osamu Kinoshita Uichi Ikeda 《Hypertension research》2006,29(2):123-128
The phosphodiesterase-5 (PDE-5) inhibitor, sildenafil, has been reported to produce sustained pulmonary vasodilatation in patients with pulmonary hypertension (PH). Recently, vardenafil, a more potent and selective PDE-5 inhibitor than sildenafil, has been approved for the treatment of erectile dysfunction. However, the long-term effects of oral vardenafil in patients with PH are unknown. We studied five consecutive patients with PH; one with primary pulmonary hypertension, two with chronic pulmonary thromboembolism, one with Eisenmenger syndrome (ventricular septal defect) and one with secondary pulmonary hypertension after a ventricular septal defect closure operation. In an acute hemodynamic trial, vardenafil (5 mg) significantly decreased both the pulmonary vascular resistance (PVR) and systemic vascular resistance (SVR) with an increase in cardiac output. In a chronic hemodynamic trial, the maintenance dose of vardenafil (10 to 15 mg) for 3 months significantly decreased the PVR, but not the SVR, with a 20.7% reduction of the PVR/ SVR ratio. Plasma brain natriuretic peptide (BNP) levels were also significantly decreased after 3 months. This pilot study demonstrates that long-term oral vardenafil therapy may be a safe and effective treatment for patients with PH. 相似文献
2.
Matsukawa T.; Hanagata K.; Ozaki M.; Iwashita H.; Koshimizu M.; Kumazawa T. 《British journal of anaesthesia》1997,78(4):396-399
We tested the hypothesis that premedication with i.m. midazolam decreases
core temperature dose-dependently. We studied six male volunteers, in
random order, on 3 days: (1) no midazolam administration (control day), (2)
midazolam 0.025 mg kg-1 i.m., (3) midazolam 0.075 mg kg-1 i.m. On the first
day, subjects were maintained alert during a 30- min control period. On the
second and third days, midazolam 0.025 or 0.075 mg kg-1 was administered
i.m. Core temperatures were measured at the right tympanic membrane. Four
adhesive skin surface probes were fixed on the chest, upper right arm,
lateral calf and thigh. Finger tip perfusion was evaluated using forearm
minus fingertip and calf minus toe, skin surface temperature gradients.
Thirty minutes after midazolam i.m., the level of sedation in the
volunteers was assessed. Peripheral venous blood was obtained immediately
after the assessment of the level of sedation. Tympanic membrane
temperatures after administration of midazolam 0.075 mg kg-1 i.m. were
significantly lower than those on the control and midazolam 0.025 mg kg-1
i.m. days at 20 and 30 min. The decreases in tympanic membrane temperatures
at 30 min after midazolam i.m. became larger as the volunteers were more
deeply sedated. i.m. midazolam produced a concentration-dependent decrease
in tympanic membrane temperature at 30 min after midazolam 0.025 and 0.075
mg kg-1 i.m. We conclude that midazolam impaired tonic thermoregulatory
vasoconstriction, allowing core-to-peripheral heat redistribution in a
dose-dependent manner after i.m. administration.
相似文献
3.
Repair of infarcted myocardium mediated by transplanted bone marrow-derived CD34+ stem cells in a nonhuman primate model 总被引:6,自引:0,他引:6
Yoshioka T Ageyama N Shibata H Yasu T Misawa Y Takeuchi K Matsui K Yamamoto K Terao K Shimada K Ikeda U Ozawa K Hanazono Y 《Stem cells (Dayton, Ohio)》2005,23(3):355-364
Rodent and human clinical studies have shown that transplantation of bone marrow stem cells to the ischemic myocardium results in improved cardiac function. In this study, cynomolgus monkey acute myocardial infarction was generated by ligating the left anterior descending artery, and autologous CD34(+) cells were transplanted to the peri-ischemic zone. To track the in vivo fate of transplanted cells, CD34(+) cells were genetically marked with green fluorescent protein (GFP) using a lentivirus vector before transplantation (marking efficiency, 41% on average). The group receiving cells (n = 4) demonstrated improved regional blood flow and cardiac function compared with the saline-treated group (n =4) at 2 weeks after transplant. However, very few transplanted cell-derived, GFP-positive cells were found incorporated into the vascular structure, and GFP-positive cardiomyocytes were not detected in the repaired tissue. On the other hand, cultured CD34(+) cells were found to secrete vascular endothelial growth factor (VEGF), and the in vivo regional VEGF levels showed a significant increase after the transplantation. These results suggest that the improvement is not the result of generation of transplanted cell-derived endothelial cells or cardiomyocytes; and raise the possibility that angiogenic cytokines secreted from transplanted cells potentiate angiogenic activity of endogenous cells. 相似文献
4.
5.
Masafumi Takahashi Uichi Ikeda Kazuyuki Shimada Tadashi Kasahara Sei-Ichi Kitagawa Yu-Ichi Takahashi Chikao Morimoto Shogo Kano Jun-Ichi Masuyama 《Journal of clinical immunology》1997,17(1):53-62
Interleukin-8 (IL-8) is a chemokine for polymorphonuclear leukocytes (PMNs) and lymphocytes, which promotes the extravasation of these inflammatory cells. In this study, we investigated IL-8 synthesis induced by the adhesive interaction between monocytes and endothelial cells during transmigration and the capacity of transmigrated monocytes to produce IL-8. Cocultured human monocytes and human umbilical vein endothelial cell (HUVEC) monolayers induced the synefgistic production of IL-8, compared with cultures of either monocytes or HUVEC monolayers alone. Coculture-induced IL-8 production almost doubled after HUVECs were stimulated with IL-1. The induced IL-8 mRNA expression was consistent with the protein data, indicating the de novo synthesis of IL-8 by the coculture. Monoclonal antibodies (mAbs) against IL-8 inhibited the transendothelial chemotactic activity of the supernatants for PMNs by 55%. Immunohistochemistry revealed that both adherent and transmigrated monocytes and unstimulated HUVECs expressed IL-8 protein, whereas nonadherent monocytes did little. Transmigrated monocytes spontaneously secreted a 3.8-fold greater amount of IL-8 than the initial monocytes. Coculture-induced IL-8 production was inhibited about 30% by polyclonal Abs against IL-, IL-1, or tumor necrosis factor , while it was not affected by mAbs against intercellular adhesion molecule 1 or vascular cell adhesion molecule 1. The results suggested that adhesive interaction during the transmigration of monocytes through HUVEC monolayers activates both cell types to produce IL-8 and that transmigrated monocytes are capable of producing ample IL-8. 相似文献
6.
Citrus auraptene inhibits chemically induced colonic aberrant crypt foci in male F344 rats 总被引:7,自引:3,他引:7
Tanaka T; Kawabata K; Kakumoto M; Makita H; Hara A; Mori H; Satoh K; Hara A; Murakami A; Kuki W; Takahashi Y; Yonei H; Koshimizu K; Ohigashi H 《Carcinogenesis》1997,18(11):2155-2161
The modifying effect of dietary administration of auraptene isolated from
the peel of citrus fruit (Citrus natsudaidai Hayata) on the development of
azoxymethane (AOM)-induced colonic aberrant crypt foci (ACF) was
investigated in rats. Male F344 rats were given s.c. injections of AOM (15
mg/kg body wt) once a week for 3 weeks to induce ACF. They also received
diets containing 100 or 500 p.p.m. auraptene for 5 weeks, starting 1 week
before the first dose of AOM. At termination of the study (week 5) dietary
administration of auraptene caused a significant reduction in the frequency
of ACF in a dose- dependent manner (P < 0.05). Feeding of auraptene
suppressed expression of cell proliferation biomarkers
(5-bromo-2'-deoxyuridine labeling- index, ornithine decarboxylase activity,
polyamine content and number of silver stained nucleolar organizer region
protein particles) in the colonic mucosa and the occurrence of micronuclei
caused by AOM. Also, auraptene increased the activities of phase II enzymes
(glutathione S- transferase and quinone reductase) in the liver and colon.
These findings might suggest that inhibition of AOM-induced ACF may be
associated, in part, with increased activity of phase II enzymes in the
liver and colon and suppression of cell proliferation in the colonic
mucosa.
相似文献
7.
Mai Shimura Hiroshi Ishikawa Hiromi Nagase Akihiko Mochizuki Futoshi Sekiguchi Naho Koshimizu Toshiyuki Itai Mizuha Odagami 《Congenital anomalies》2018,58(5):167-170
We investigated whether it was possible to predict the prognosis of fetuses with cystic hygroma in early pregnancy based on the degree of neck thickening. We retrospectively analyzed 57 singleton pregnancies with fetuses with cystic hygroma who were examined before the 22nd week of pregnancy. The fetuses were categorized according to the outcome, structural abnormalities at birth, and chromosomal abnormalities. Here, we proposed a new sonographic predictor with which we assessed neck thickening by dividing the width of the neck thickening by the biparietal diameter, which is expressed as the cystic hygroma width/biparietal diameter ratio. The median cystic hygroma width/biparietal diameter ratio in the intrauterine fetal death group (0.51) was significantly higher than that in the live birth group (0.27). No significant difference in the median cystic hygroma width/biparietal diameter ratio was found between the structural abnormalities group at birth and the no structural abnormalities group, and no significant difference in the median cystic hygroma width/biparietal diameter ratio was found between the chromosomal abnormality group and the no chromosomal abnormality group. We used receiver operating characteristic analysis to evaluate the cystic hygroma width/biparietal diameter ratio to predict intrauterine fetal death. When the cystic hygroma width/biparietal diameter ratio cut‐off value was 0.5, intrauterine fetal death could be predicted with a sensitivity of 52.9% and a specificity of 100%. It is possible to predict intrauterine fetal death in fetuses with cystic hygroma in early pregnancy if cystic hygroma width/biparietal diameter ratio is measured. However, even if cystic hygroma width/biparietal diameter ratio is measured, predicting the presence or absence of a structural abnormality at birth or a chromosomal abnormality is difficult. 相似文献
8.
T Tono T Tsujimura U Koshimizu T Kasugai S Adachi K Isozaki S Nishikawa M Morimoto Y Nishimune S Nomura 《Blood》1992,80(6):1448-1453
9.
Pituitary cells secrete ATP, which acts as an autocrine and/or paracrine extracellular messenger on two families of purinergic receptors: G-protein-coupled P2Y receptors (P2YRs) and ion-conducting P2X receptors (P2XRs). Lactotrophs and GH(3)-immortalized cells express the P2Y(2)R subtype. Several P2XR subtypes are expressed in pituitary cells. Gonadotrophs and somatotrophs express P2X(2a)R and P2X(2b)R, which occur as heteromeric channels. Lactotrophs and GH(3) cells express one or more ion-conducting subtypes from among P2X(3)R, P2X(4)R and P2X(7)R in homomeric form. Thyrotrophs and corticotrophs also express P2XRs, but their identification requires further study. Pituitary cells express purinergic P1 receptors, which are activated by adenosine. The A(1)R subtype of these receptors is expressed in melanotrophs and GH(3) cells. In this review, we briefly discuss the expression and coupling of A(1)R and P2Y(2)R, and focus on the expression and Ca(2+) signaling of P2XRs. 相似文献
10.
Yukinori Nakagawa Uichi Ikeda Masahiro Hirose Satoshi Ubukata Taka-Aki Katsuki Yuichiro Kaminishi Tsutomu Saito Mitsugu Hironaka Tohru Izumi Kazuyuki Shimada 《Circulation journal》2004,68(2):172-173
Primary cardiac malignant lymphoma is extremely rare and almost all patients die within weeks. Monoclonal CD20 antibody (rituximab) was administered to a patient with primary cardiac B-cell non-Hodgkin's lymphoma expressing a CD20 molecule. The results suggest that rituximab may be a safe and effective new therapy for primary cardiac B-cell lymphoma. 相似文献