全文获取类型
收费全文 | 3961篇 |
免费 | 244篇 |
国内免费 | 50篇 |
专业分类
耳鼻咽喉 | 46篇 |
儿科学 | 197篇 |
妇产科学 | 94篇 |
基础医学 | 438篇 |
口腔科学 | 81篇 |
临床医学 | 302篇 |
内科学 | 957篇 |
皮肤病学 | 173篇 |
神经病学 | 285篇 |
特种医学 | 236篇 |
外科学 | 608篇 |
综合类 | 50篇 |
一般理论 | 5篇 |
预防医学 | 268篇 |
眼科学 | 31篇 |
药学 | 269篇 |
中国医学 | 5篇 |
肿瘤学 | 210篇 |
出版年
2023年 | 15篇 |
2022年 | 30篇 |
2021年 | 85篇 |
2020年 | 43篇 |
2019年 | 72篇 |
2018年 | 80篇 |
2017年 | 84篇 |
2016年 | 76篇 |
2015年 | 83篇 |
2014年 | 103篇 |
2013年 | 133篇 |
2012年 | 185篇 |
2011年 | 184篇 |
2010年 | 164篇 |
2009年 | 125篇 |
2008年 | 172篇 |
2007年 | 189篇 |
2006年 | 170篇 |
2005年 | 216篇 |
2004年 | 186篇 |
2003年 | 177篇 |
2002年 | 169篇 |
2001年 | 149篇 |
2000年 | 137篇 |
1999年 | 125篇 |
1998年 | 88篇 |
1997年 | 67篇 |
1996年 | 68篇 |
1995年 | 45篇 |
1994年 | 57篇 |
1993年 | 45篇 |
1992年 | 65篇 |
1991年 | 60篇 |
1990年 | 59篇 |
1989年 | 60篇 |
1988年 | 77篇 |
1987年 | 49篇 |
1986年 | 35篇 |
1985年 | 59篇 |
1984年 | 22篇 |
1983年 | 14篇 |
1982年 | 18篇 |
1981年 | 15篇 |
1980年 | 16篇 |
1979年 | 20篇 |
1977年 | 16篇 |
1976年 | 18篇 |
1972年 | 15篇 |
1970年 | 16篇 |
1969年 | 17篇 |
排序方式: 共有4255条查询结果,搜索用时 109 毫秒
1.
2.
Alexander Stoff MD ; Angel A. Rivera PhD ; N. S. Banerjee PhD ; J. Michael Mathis PhD ; Antonio Espinosa-de-los-Monteros MD ; Long P. Le PhD ; Jorge I. De la Torre MD ; Luis O. Vasconez MD ; Thomas R. Broker PhD ; Dirk F. Richter MD ; Mariam A. Stoff-Khalili MD ; David T. Curiel MD PhD 《Wound repair and regeneration》2006,14(5):608-617
Genetically modified keratinocytes and fibroblasts are suitable for delivery of therapeutic genes capable of modifying the wound healing process. However, efficient gene delivery is a prerequisite for successful gene therapy of wounds. Whereas adenoviral vectors (Ads) exhibit superior levels of in vivo gene transfer, their transductional efficiency to cells resident within wounds may nonetheless be suboptimal, due to deficiency of the primary adenovirus receptor, coxsackie-adenovirus receptor (CAR). We explored CAR-independent transduction to fibroblasts and keratinocytes using a panel of CAR-independent fiber-modified Ads to determine enhancement of infectivity. These fiber-modified adenoviral vectors included Ad 3 knob (Ad5/3), canine Ad serotype 2 knob (Ad5CAV-2), RGD (Ad5.RGD), polylysine (Ad5.pK7), or both RGD and polylysine (Ad5.RGD.pK7). To evaluate whether transduction efficiencies of the fiber-modified adenoviral vectors correlated with the expression of their putative receptors on keratinocytes and fibroblasts, we analyzed the mRNA levels of CAR, alpha upsilon integrin, syndecan-1, and glypican-1 using quantitative polymerase chain reaction. Analysis of luciferase and green fluorescent protein transgene expression showed superior transduction efficiency of Ad5.pK7 in keratinocytes and Ad5.RGD.pK7 in fibroblasts. mRNA expression of alpha upsilon integrin, syndecan-1 and glypican-1 was significantly higher in primary fibroblasts than CAR. In keratinocytes, syndecan-1 expression was significantly higher than all the other receptors tested. Significant infectivity enhancement was achieved in keratinocytes and fibroblasts using fiber-modified adenoviral vectors. These strategies to enhance infectivity may help to achieve higher clinical efficacy of wound gene therapy. 相似文献
3.
4.
5.
6.
M Ardigo I Malizewsky ML Dell'Anna E Berardesca M Picardo 《Journal of the European Academy of Dermatology and Venereology》2007,21(10):1344-1350
BACKGROUND: Vitiligo is the most common pigmentary disorder with a global incidence from 0.1% to 2% in different geographical areas. Histopathology and histochemistry have shown the reduction of melanocytes in achromic patches, but microscopic changes of lesional and non-lesional skin are still not completely understood. Reflectance confocal microscopy (RCM), based on the different light reflectance index of cutaneous structures, allowed in vivo, en face microscopic evaluation of superficial skin layers with a resolution similar to skin histology. AIM: The purpose of this study was to evaluate RCM features of lesional and non-lesional skin of vitiligo patients. Moreover, re-pigmented areas were taken into consideration in order to evaluate melanocyte response to ultraviolet B (UVB) radiation. SUBJECTS AND METHODS: Sixteen patients of different phototypes affected by active non-segmental vitiligo and 10 controls were enrolled in the study. In vivo skin imaging was done using a commercially available RCM (Lucid, Vivascope 1500. Re-pigmented areas from 6 to 16 patients (after UVB narrow-band therapy) were also examined. RESULTS: Vitiligo lesions showed the disappearance of the bright rings normally seen at the dermo-epidermal junction. Moreover, non-lesional skin of vitiligo patients showed unexpected changes as the presence of half-rings or scalloped border-like features of the bright papillary rings. In re-pigmented areas after UVB narrow band therapy, the presence of activated, dendritic melanocytes was seen. CONCLUSIONS: Considering our results, and following further studies, RCM clinical applications could be used in the therapeutic monitoring and evaluation of the evolution of vitiligo. 相似文献
7.
8.
A 22-year-old man who had previously undergone work-up for right cryptorchidism and been diagnosed as having right testicular absence was referred for repair of a right inguinal hernia. A laparoscopic approach was chosen for this patient, and at the time of laparoscopic herniorrhaphy, a small intraabdominal testicle was visualized and removed laparoscopically.Laparoscopy has served as an excellent procedure for locating intraabdominal testes and planning the most appropriate treatment for many cryptorchid patients. Until recently, atrophic testes located within the abdominal cavity through use of a laparoscope were removed via an open technique. Advances in laparoscopy now enable detection and definitive treatment for these patients without the need for large, more painful incisions.The laparoscopic approach to this patient enabled diagnosis and management of his cryptorchidism and provided a means for repair of his hernia. 相似文献
9.
10.
M. Spada W. Petz A. Bertani S. Riva A. Sonzogni M. Giovannelli E. Torri G. Torre M. Colledan B. Gridelli 《American journal of transplantation》2006,6(8):1913-1921
Avoidance of corticosteroids could be beneficial after pediatric liver transplantation (LTx). To test this hypothesis, we performed a randomized prospective study to compare immunosuppression with tacrolimus (TAC) and steroids versus TAC and basiliximab (BAS) after pediatric LTx. Seventy-two patients were recruited, 36 receiving TAC and steroids and 36 TAC and BAS. The primary endpoint was the occurrence of the first rejection episode. Secondary endpoints were the cumulative incidence and severity of rejection, patient and graft survival, and incidence of adverse events. Overall 1-year patient and graft survival rates were 91.4% and 85.5% in the steroid group, and 88.6% and 80% in the BAS group (p = NS). Patients free from rejection were 87.7% in the BAS group and 67.7% in the steroid group (p = 0.036). The use of BAS was associated with a 63.6% reduction in incidence of acute rejection episodes. Overall incidence of infection was 72.3% in the steroid group and 50% in the BAS group (p = 0.035). We conclude that the combination of TAC with BAS is an alternative to TAC and steroid immunosuppression in pediatric LTx, which allows for a significant reduction in the incidence of acute rejection and infectious complications. 相似文献