Bovine β-Lactoglobulin in the Human Milk

ABSTRACT. Human milk samples ( n =232) collected during the whole lactation period from 25 healthy, Swedish mothers were analyzed by radioimmunologic method for content of bovine β-lacto-globulin. Detectable amounts (5-800 μ/1) were found in 93 of 232 milk samples (40%). Six mothers had no detectable β-lactoglobulin in their breast milk on any occasion. Two mothers had measurable /Mactoglobulin in all their milk samples. No correlation was found between daily cow's milk intake and concentration of β-lactoglobulin in the milk samples. Six mothers with allergic symptoms such as asthma, hay-fever, eczema all had detectable amounts of β-lactoglobulin in their milk. Of 19 mothers without allergy, 13 had detectable amounts. This difference did not show statistical significance. The presence of symptoms in the infant such as diarrhoea, vomiting, colic, exanthema was significantly correlated to high levels of β-lactoglobulin in the milk. Bovine β-lactoglobulin was also detected in 7 of 13 serum samples. The two mothers with detectable β-lactoglobulin in all milk samples had the highest serum values, and their infants suffered from gastro-intestinal symptoms, weight decline and exanthema.     

Iron staining of the acquired enamel pellicle after exposure to tannic acid or chlorhexidine: preliminary report

Abstract – Extrinsic discoloration of teeth following a large consumption of tannin-containing beverages or a prolonged use of chlorhexidine mouthrinses is a well known observation. Tannins as well as chlorhexidine are denaturing agents. Based on preliminary studies revealing the presence of iron in chlorhexidine discolored pellicle material, the ability of iron to stain the integument after pretreatmentwith the two denaturants was studied in a human model. The denaturing effect of an acidic environment was also included. Enamel slabs fixedto acrylie appliances were carried in the oral cavity and alternately exposed to the test solutions in different sequences in vitro. Pretreatment with chlorhexidine or tannic acid led to marked discolorations upon iron application during 5-d tests, whereas the compounds individually had no such effect. A large content of the metal was found in the stained material. Stannous fluoride appeared to reduce the formation of the pigments, and strong oxidation completely bleached the established color. Possible mechanisms underlying the phenomena observed are discussed.     

Presence in dental plaque of a polysaccharide antigen of the anaerobic oral filamentousorganism Eubacterium saburreum

ABSTRACT – Plaque samples were examined by double diffusion in agar for the presence of PS L44, a surface antigen of Eubacterium saburreum . The antigen was detected in eight ofnine samples     

Biological activity of lipopolysaccharides from oral Selenomonas

Abstract – Lipopolysaccharides (LPSs) were extracted by phenol-water From three oral strains of Selenomonas. The preparations were tested for the ability to induce a blastogenic response in cultures of spleen cells from normal and nude BALB/c mice, to activate guinea pig complement and the clotting enzyme system of Limulus polyphemus amoebocytes, and to kill Actinomycin-D treated mice. The capacity of the three LPSs was comparable to that of enterobacterial LPS.     

SYSTEMIC REACTION TO SUBARACHNOID INJECTION OF PHENYLEPHRINE

An adverse reaction is presented. The patient developed a skinrash, hypertension, bradycardia, a ventricular bigeminal rhythm,and signs of ischaemia in the electrocardiogram after subarachnoidinjection of phenyl-ephrine 10 mg     

Heavy drinking in the restaurant business: the role of social modelling and structural factors of the work-place

The role of social modelling and structural factors of the work-place in predicting the probability of heavy drinking was investigated in a sample of 3267 Norwegian male and female waiters and cooks. In the logistic regression analysis, the probability of heavy drinking was increased by two social modelling factors and one structural factor. Having co-workers who, at least weekly, took an end-of-work drink at the work-place gave an odds ratio for heavy drinking of 2.8 (95% CI1.9–4.1), and having co-workers who went out after work at least every week gave an odds ratio of 1.8 (95% CI 1.2–2.8). Working at a place with a liberal alcohol policy gave an odds ratio 1.5 (95% CI 1.1–2.2). Among the background factors, only household type significantly predicted heavy drinking. As compared with living with children, the odds for heavy drinking when living alone was 4.3 (95% CI 2.9–6.4). The results indicate that preventive measures in the restaurant business should not only concentrate on the individual, but also deal with factors related to the occupational activity that promote and sustain heavy drinking.