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排序方式: 共有96条查询结果,搜索用时 15 毫秒
1.
Rapid detection of Salmonella enterica serovar Choleraesuis in blood cultures by a dot blot enzyme-linked immunosorbent assay 总被引:1,自引:0,他引:1
Janyapoon K Korbsrisate S Thamapa H Thongmin S Kanjanahareutai S Wongpredee N Sarasombath S 《Clinical and diagnostic laboratory immunology》2000,7(6):977-979
A dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella enterica serovar Choleraesuis in blood cultures. This system was applied to the identification of serovar Choleraesuis, and the results were compared with those obtained by a conventional biochemical method. It was revealed that all 12 samples identified to be infected with serovar Choleraesuis were positive on testing by the ELISA. In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%. 相似文献
2.
Buranapraditkun S Nookhai S Ubolyam S Sirivichayakul S Phanuphak P Ruxrungtham K 《Asian Pacific journal of allergy and immunology / launched by the Allergy and Immunology Society of Thailand》2007,25(1):83-89
In Thailand, the cost of antiretrovirals has recently been reduced more than 10 fold. Likewise strategies for a cost reduction in laboratory monitoring are warranted. This study was designed to explore if the most expensive reagent in flow cytometry based CD4+ cell monitoring, the CD4+/CD8+ monoclonal antibodies, can be reduced without a loss of accuracy. Blood samples from 55 HIV seronegative (HIV-) and 76 HIV+ subjects were analyzed for %CD4+ and %CD8+ T cells using a two color monoclonal antibody panel (BD Biosciences, CA, USA) with 3 different amounts of the recommended reagents for staining: 1) standard, 2) half, and 3) one-fourth. A significant Spearman correlation of 0.987 was shown for the % CD4+ T cell test results for one half as well as one-fourth of the recommended amount compared to the standard staining according to the manufacturer's instruction (p < 0.0001). For the % CD8+ T cell test results, the correlation between the standard and the half or one-fourth reduced staining was 0.972 (p < 0.0001). Bland-Altman analysis showed no significant bias between the results from one half or one-fourth of the recommended amount versus the standard. The sensitivity and specificity of the two methods at the CD4+ T cell count cut-off of 200 cells/microl were 93% and 100%; and 96% and 99%, respectively. Our study indicates that a reduction of the reagents to half or one-fourth of the amount recommended by the manufacturer was still able to generate reliable results for CD4+ and CD8+ T cell counts. Such an approach will significantly reduce the cost of CD4+ monitoring for resource limited settings where a flow cytometer is available. 相似文献
3.
James W. Harrison Tran Thi Ngoc Dung Fariha Siddiqui Sunee Korbrisate Habib Bukhari My Phan Vu Tra Nguyen Van Minh Hoang Juan Carrique-Mas Juliet Bryant James I. Campbell David J. Studholme Brendan W. Wren Stephen Baker Richard W. Titball Olivia L. Champion 《Emerging infectious diseases》2014,20(6):1026-1029
A novel protein translocation system, the type-6 secretion system (T6SS), may play a role in virulence of Campylobacter jejuni. We investigated 181 C. jejuni isolates from humans, chickens, and environmental sources in Vietnam, Thailand, Pakistan, and the United Kingdom for T6SS. The marker was most prevalent in human and chicken isolates from Vietnam. 相似文献
4.
Benchaporn Aneksan Montawan Sawatdipan Sunee Bovonsunthonchai Jarugool Tretriluxana Roongtiwa Vachalathiti Paradee Auvichayapat Anuchai Pheungphrarattanatrai Pagamas Piriyaprasarth Wanalee Klomjai 《Neuromodulation》2022,25(4):558-568
ObjectiveTo determine the effect of five-session dual-transcranial direct current stimulation (dual-tDCS) combined with task-specific training on gait and lower limb motor performance in individuals with subacute stroke.Materials and MethodsTwenty-five participants who had a stroke in the subacute phase with mild motor impairment were recruited, randomized, and allocated into two groups. The active group (n = 13) received dual-tDCS with anodal over the lesioned hemisphere M1 and cathodal over the nonlesioned hemisphere, at 2 mA for 20 min before training for five consecutive days, while the sham group (n = 12) received sham mode before training. Gait speed as a primary outcome, temporospatial gait variables, lower-limb functional tasks (sit-to-stand and walking mobility), and muscle strength as secondary outcomes were collected at preintervention and postintervention (day 5), one-week follow-up, and one-month follow-up.ResultsThe primary outcome and most of the secondary outcomes were improved in both groups, with no significant difference between the two groups, and most of the results indicated small to moderate effect sizes of active tDCS compared to sham tDCS.ConclusionThe combined intervention showed no benefit over training alone in improving gait variables and lower-limb performance. However, some performances were saturated at some point, as moderate to high function participants were recruited in the present study. Future studies should consider recruiting participants with more varied motor impairment levels and may need to determine the optimal stimulation protocols and parameters to improve gait and lower-limb performance. 相似文献
5.
Nucleoside analogue mutations and Q151M in HIV-1 subtype A/E infection treated with nucleoside reverse transcriptase inhibitors 总被引:1,自引:0,他引:1
6.
Tadech Boonpiyathad Willem van de Veen Oliver Wirz Milena Sokolowska Beate Rückert Ge Tan Atik Sangasapaviliya Panitan Pradubpongsa Rattanaporn Fuengthong Pattarawat Thantiworasit Sunee Sirivichayakul Kiat Ruxrungtham Cezmi A. Akdis Mübeccel Akdis 《The Journal of allergy and clinical immunology》2019,143(3):1077-1086.e10
7.
Sriphrapradang Chutintorn Pinyopodjanard Sittichai Suntornlohanakul Onnicha Nimitphong Hataikarn Chirakalwasan Naricha Saetang Sunee Anothaisintawee Thunyarat Siwasaranond Nantaporn Manodpitipong Areesa Chailurkit La-Or Reutrakul Sirimon 《Sleep & breathing》2019,23(3):963-967
Sleep and Breathing - Hypothyroidism is associated with a high frequency of obstructive sleep apnea (OSA). However, the prevalence of OSA in hypothyroid patients is not different from the general... 相似文献
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10.
Panan Pichayapinyo PhD Sunee Lagampan EdD Nichaphat Rueangsiriwat RN MSc 《International journal of nursing practice》2015,21(3):278-285
The purpose of this study was to investigate the effect of a dietary modification intervention programme by applying the Stages of Change Model in 2 h postprandial capillary glucose reduction among Thai population. A randomized control trial was conducted for people at risk of type 2 diabetes for a period of 8 weeks. The intervention programme consisted of evaluating an individual's stage of change after being provided dietary information regarding kind of food and portions, discussion with a role model, and keeping a food diary record. By the end of the intervention programme, most participants in the experimental group were in the action stage (n = 36), whereas those in the control group were in the preparation stage (n = 32). Body mass index, blood pressure, food consumption behaviour and the 2 h postprandial blood glucose (PPG) in the experimental group had improved (P < 0.05). When performing regression analysis, intervention participation and the 2 h PPG at the baseline accounted for approximately 54% of total variance in predicting the 2 h PPG. This study yielded evidence for the benefits of using the Stages of Change Model as a framework in a dietary modification programme among people at risk of type 2 diabetes. 相似文献