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排序方式: 共有96条查询结果,搜索用时 78 毫秒
1.
Rapid detection of Salmonella enterica serovar Choleraesuis in blood cultures by a dot blot enzyme-linked immunosorbent assay 总被引:1,自引:0,他引:1
Janyapoon K Korbsrisate S Thamapa H Thongmin S Kanjanahareutai S Wongpredee N Sarasombath S 《Clinical and diagnostic laboratory immunology》2000,7(6):977-979
A dot blot enzyme-linked immunosorbent assay (ELISA) with a monoclonal antibody specific to phase1-c Salmonella was developed for the direct detection of Salmonella enterica serovar Choleraesuis in blood cultures. This system was applied to the identification of serovar Choleraesuis, and the results were compared with those obtained by a conventional biochemical method. It was revealed that all 12 samples identified to be infected with serovar Choleraesuis were positive on testing by the ELISA. In contrast, 77 samples infected with bacteria commonly isolated from the blood were not reactive by the ELISA. The calculated sensitivity and specificity of the established assay are 100%. 相似文献
2.
Tadech Boonpiyathad Willem van de Veen Oliver Wirz Milena Sokolowska Beate Rückert Ge Tan Atik Sangasapaviliya Panitan Pradubpongsa Rattanaporn Fuengthong Pattarawat Thantiworasit Sunee Sirivichayakul Kiat Ruxrungtham Cezmi A. Akdis Mübeccel Akdis 《The Journal of allergy and clinical immunology》2019,143(3):1077-1086.e10
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Buranapraditkun S Nookhai S Ubolyam S Sirivichayakul S Phanuphak P Ruxrungtham K 《Asian Pacific journal of allergy and immunology / launched by the Allergy and Immunology Society of Thailand》2007,25(1):83-89
In Thailand, the cost of antiretrovirals has recently been reduced more than 10 fold. Likewise strategies for a cost reduction in laboratory monitoring are warranted. This study was designed to explore if the most expensive reagent in flow cytometry based CD4+ cell monitoring, the CD4+/CD8+ monoclonal antibodies, can be reduced without a loss of accuracy. Blood samples from 55 HIV seronegative (HIV-) and 76 HIV+ subjects were analyzed for %CD4+ and %CD8+ T cells using a two color monoclonal antibody panel (BD Biosciences, CA, USA) with 3 different amounts of the recommended reagents for staining: 1) standard, 2) half, and 3) one-fourth. A significant Spearman correlation of 0.987 was shown for the % CD4+ T cell test results for one half as well as one-fourth of the recommended amount compared to the standard staining according to the manufacturer's instruction (p < 0.0001). For the % CD8+ T cell test results, the correlation between the standard and the half or one-fourth reduced staining was 0.972 (p < 0.0001). Bland-Altman analysis showed no significant bias between the results from one half or one-fourth of the recommended amount versus the standard. The sensitivity and specificity of the two methods at the CD4+ T cell count cut-off of 200 cells/microl were 93% and 100%; and 96% and 99%, respectively. Our study indicates that a reduction of the reagents to half or one-fourth of the amount recommended by the manufacturer was still able to generate reliable results for CD4+ and CD8+ T cell counts. Such an approach will significantly reduce the cost of CD4+ monitoring for resource limited settings where a flow cytometer is available. 相似文献
4.
James W. Harrison Tran Thi Ngoc Dung Fariha Siddiqui Sunee Korbrisate Habib Bukhari My Phan Vu Tra Nguyen Van Minh Hoang Juan Carrique-Mas Juliet Bryant James I. Campbell David J. Studholme Brendan W. Wren Stephen Baker Richard W. Titball Olivia L. Champion 《Emerging infectious diseases》2014,20(6):1026-1029
A novel protein translocation system, the type-6 secretion system (T6SS), may play a role in virulence of Campylobacter jejuni. We investigated 181 C. jejuni isolates from humans, chickens, and environmental sources in Vietnam, Thailand, Pakistan, and the United Kingdom for T6SS. The marker was most prevalent in human and chicken isolates from Vietnam. 相似文献
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Nucleoside analogue mutations and Q151M in HIV-1 subtype A/E infection treated with nucleoside reverse transcriptase inhibitors 总被引:1,自引:0,他引:1
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Panan Pichayapinyo PhD Sunee Lagampan EdD Nichaphat Rueangsiriwat RN MSc 《International journal of nursing practice》2015,21(3):278-285
The purpose of this study was to investigate the effect of a dietary modification intervention programme by applying the Stages of Change Model in 2 h postprandial capillary glucose reduction among Thai population. A randomized control trial was conducted for people at risk of type 2 diabetes for a period of 8 weeks. The intervention programme consisted of evaluating an individual's stage of change after being provided dietary information regarding kind of food and portions, discussion with a role model, and keeping a food diary record. By the end of the intervention programme, most participants in the experimental group were in the action stage (n = 36), whereas those in the control group were in the preparation stage (n = 32). Body mass index, blood pressure, food consumption behaviour and the 2 h postprandial blood glucose (PPG) in the experimental group had improved (P < 0.05). When performing regression analysis, intervention participation and the 2 h PPG at the baseline accounted for approximately 54% of total variance in predicting the 2 h PPG. This study yielded evidence for the benefits of using the Stages of Change Model as a framework in a dietary modification programme among people at risk of type 2 diabetes. 相似文献
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Arkaravichien Wiwat Wongpratat Apichaya Lertsinudom Sunee 《International journal of clinical pharmacy》2016,38(4):899-907
International Journal of Clinical Pharmacy - Background Quality indicators determine the quality of actual practice in reference to standard criteria. The Community Pharmacy Association (Thailand),... 相似文献