全文获取类型
收费全文 | 294篇 |
免费 | 23篇 |
国内免费 | 1篇 |
专业分类
儿科学 | 12篇 |
妇产科学 | 3篇 |
基础医学 | 36篇 |
口腔科学 | 2篇 |
临床医学 | 31篇 |
内科学 | 81篇 |
皮肤病学 | 10篇 |
神经病学 | 12篇 |
特种医学 | 7篇 |
外科学 | 35篇 |
综合类 | 8篇 |
预防医学 | 19篇 |
眼科学 | 2篇 |
药学 | 32篇 |
中国医学 | 1篇 |
肿瘤学 | 27篇 |
出版年
2023年 | 1篇 |
2022年 | 2篇 |
2021年 | 4篇 |
2020年 | 5篇 |
2019年 | 6篇 |
2018年 | 12篇 |
2017年 | 4篇 |
2016年 | 8篇 |
2015年 | 8篇 |
2014年 | 7篇 |
2013年 | 10篇 |
2012年 | 23篇 |
2011年 | 23篇 |
2010年 | 8篇 |
2009年 | 11篇 |
2008年 | 12篇 |
2007年 | 21篇 |
2006年 | 15篇 |
2005年 | 11篇 |
2004年 | 13篇 |
2003年 | 13篇 |
2002年 | 5篇 |
2001年 | 11篇 |
2000年 | 6篇 |
1999年 | 1篇 |
1998年 | 10篇 |
1997年 | 7篇 |
1996年 | 2篇 |
1995年 | 6篇 |
1994年 | 4篇 |
1993年 | 3篇 |
1992年 | 2篇 |
1991年 | 4篇 |
1990年 | 7篇 |
1988年 | 3篇 |
1987年 | 2篇 |
1986年 | 3篇 |
1985年 | 3篇 |
1984年 | 2篇 |
1981年 | 2篇 |
1980年 | 3篇 |
1979年 | 2篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1974年 | 3篇 |
1973年 | 2篇 |
1972年 | 3篇 |
1969年 | 1篇 |
1968年 | 1篇 |
排序方式: 共有318条查询结果,搜索用时 15 毫秒
1.
2.
Pitt-Rogers-Danks syndrome and Wolf-Hirschhorn syndrome are caused by a deletion in the same region on chromosome 4p 16.3. 总被引:2,自引:1,他引:2 下载免费PDF全文
S G Kant A Van Haeringen E Bakker I Stec D Donnai P Mollevanger G C Beverstock M C Lindeman-Kusse G J Van Ommen 《Journal of medical genetics》1997,34(7):569-572
Recently, a deletion of chromosome 4pter was found in three patients with Pitt-Rogers-Danks syndrome. We investigated two of these patients, by means of DNA and FISH studies, together with two additional patients with Pitt-Rogers-Danks syndrome, to determine the critical region of the deletion in these patients and to compare this with the critical region in Wolf-Hirschhorn syndrome. All four patients showed terminal deletions of chromosome 4p of different sizes. One of them appeared to have an unbalanced karyotype caused by a cryptic translocation t(4;8) in the mother, resulting in a deletion of chromosome 4pter and a duplication of chromosome 8pter. The localisation of the Wolf-Hirschhorn critical region has been confined to approximately 1 Mb between D4S43 and D4S115. Our study shows that the deletions in four patients with the Pitt-Rogers-Danks syndrome overlap the Wolf-Hirschhorn critical region and extend beyond this in both directions. This study, combined with the fact that our third patient, who was previously described as a Pitt-Rogers-Danks patient, but who now more closely resembles a Wolf-Hirschhorn patient, makes it likely that Pitt-Rogers-Danks and Wolf-Hirschhorn syndromes are different clinical phenotypes resulting from a deletion in the same microscopic region on chromosome 4p16. 相似文献
3.
目的 真核表达人呼吸道合胞病毒(human respiratory syncytial virus,SV)融合蛋白(fusion protein,),并完成蛋白纯化及纯度测定.方法 根据编码F蛋白的基因序列设计引物,CR方法扩增出3'端带His标签的F基因序列,克隆入pGEM-T-easy载体,经核酸序列分析后,进一步克隆到pcDNA3.1( )真核表达载体,限制性内切酶鉴定,用脂质体Lipofectamine2000转染COS-7细胞,2 h后再用Westem blot检测目的蛋白的表达.Ni柱亲和层析纯化COS-7细胞表达的F蛋白,高效毛细管电泳分析纯化后蛋白纯度.结果 核酸序列分析证实获得带His标签的RSV F基因序列,没有发生无义突变.转染COS-7细胞后,利用Western blot方法检测到F蛋白的特异性条带,纯度达99%以上.结论 初步建立了真核表达RSV F蛋白的纯化方法,为进一步优化RSV F蛋白制备条件及单克隆抗体及诊断试剂等研究奠定了基础. 相似文献
4.
5.
Event-free survival in patients after an acute coronary event with exercise-induced normalization of the T-wave 总被引:1,自引:0,他引:1
BACKGROUND: Risk stratification of patients with unstable angina or non-Q-wave myocardial infarction (MI) is an unresolved clinical problem. The prognostic value of T-wave normalization (TWN) during exercise has not been studied in this group of patients. HYPOTHESIS: Event-free survival in clinically stable patients after an acute coronary event without ST-segment elevation can be predicted by the presence of exercise-induced TWN. METHODS: Sixty-five patients (43 men and 22 women, mean age 62+/-10 years) entered the study. The diagnosis of unstable angina and non-Q-wave MI was made in 40 and 25 patients, respectively. A treadmill exercise test was performed in all patients after clinical stabilization. The patients were divided into three groups: those with negative baseline T waves and exercise-induced TWN (Group 1); those with negative baseline T waves, but without TWN (Group 2); and those with positive baseline T waves (Group 3). The patients were followed up for 6 months. RESULTS: During follow-up, serious cardiovascular complications occurred in 15 (23%) patients. These included exacerbation of ischemic heart disease (14 patients) and acute MI (1 patient). Event-free survival was greater in patients in Group 1 (95%) than in those in Group 2 (68%, p < 0.034) or Group 3 (71%, NS). Among all patients studied, exercise-induced TWN was predictive of event-free survival with a sensitivity of 38% and a specificity of 93%. CONCLUSIONS: In clinically stable patients after an acute coronary event without ST-segment elevation, exercise-induced TWN is a specific but n ot sensitive predictor of event-free survival after 6 months. 相似文献
6.
Alexey Maximenko Joanna Depciuch Natalia opuszyska Malgorzata Stec aneta
wiatkowska-Warkocka Vadim Bayev Piotr M. Zieliski Jaroslaw Baran Julia Fedotova Wadysaw P. Wglarz Magdalena Parlinska-Wojtan 《RSC advances》2020,10(44):26508
Novel functionalized (biofunctionalization followed by cisplatin immobilization) Fe3O4@SiO2@Au nanoparticles (NPs) were designed. The encapsulation of Fe3O4 cores inside continuous SiO2 shells preserves their initial structure and strong magnetic properties, while the shell surface can be decorated by small Au NPs, and then cisplatin (cPt) can be successfully immobilized on their surface. The fabricated NPs exhibit very strong T2 contrasting properties for magnetic resonance imaging (MRI). The functionalized Fe3O4@SiO2@Au NPs are tested for a potential application in photothermal cancer therapy, which is simulated by irradiation of two colon cancer cell lines (SW480 and SW620) with a laser (λ = 808 nm, W = 100 mW cm−2). It is found that the functionalized NPs possess low toxicity towards cancer cells (∼10–15%), which however could be drastically increased by laser irradiation, leading to a mortality of the cells of ∼43–50%. This increase of the cytotoxic properties of the Fe3O4@SiO2@Au NPs, due to the synergic effect between the presence of cPt plus Au NPs and laser irradiation, makes these NPs perspective agents for potential (MRI)-guided stimulated chemo-photothermal treatment of cancer.Novel functionalized nanoparticles, with toxicity controlled by laser irradiation, are perspective agents for potential (MRI)-guided stimulated chemo-photothermal treatment of cancer. 相似文献
7.
8.
Csongradi E Docarmo JM Dubinion JH Vera T Stec DE 《International journal of obesity (2005)》2012,36(2):244-253
9.
10.
Clinical usefulness of implantable loop recorder in diagnosis of unexplained syncope - case report. A case of a 67-year-old female with recurrent syncope is presented. In spite of extensive investigation, the cause of syncope remained unknown. The patient received insertable loop recorder (ILR). One month after implantation the patient suffered two syncopal episodes. ECG recorded by ILR showed asystolic pause of 8 seconds at the time of conversion of atrial fibrillation to sinus rhythm. This was the first ILR implantation in Poland which was covered by public health insurance and not by private funds. 相似文献