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1.
Common regulatory elements control symbiotic and microaerobic induction of nifA in Rhizobium meliloti. 总被引:24,自引:2,他引:22 下载免费PDF全文
E L Virts S W Stanfield D R Helinski G S Ditta 《Proceedings of the National Academy of Sciences of the United States of America》1988,85(9):3062-3065
We have previously demonstrated that the nifA promoter (nifAp) of Rhizobium meliloti is inducible under microaerobic conditions in the absence of alfalfa. Here we show that microaerobic activation of nifAp involves both cis- and trans-acting regulatory controls identical to those used symbiotically. The start site for nifA mRNA synthesis was found to be the same during symbiosis and microaerobiosis, and a deletion analysis of nifAp demonstrated that DNA between positions -62 and -45 is essential for induction. Mutants isolated as being unable to induce nifA microaerobically also were found to be defective in symbiotic nitrogen fixation with alfalfa. Such mutants form nodules that are equivalent cytologically to those induced by nifA::Tn5 mutants. Genetic and structural studies have localized the mutations to a cluster of fix genes 200 kilobases distant from the nod-nif region on the pSym megaplasmid [Renalier, M.-H., Batut, J., Ghai, J., Terzaghi, B., Gherardi, M., David, M., Garnerone, A.-M., Vasse, J., Truchet, G., Huguet, T. & Boistard, P. (1987) J. Bacteriol. 169, 2231-2238]. 相似文献
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The mammalian neocortex is functionally organized into numerous specialized "areas." The distinct functional properties characteristic of each area are in large part due to connectional and architectural differences among the areas. However, these "area-specific" features which distinguish mature areas are not apparent early in the development of neocortex. We have used heterotopic cortical transplantation to examine whether these area-specific features are prespecified or emerge as a result of epigenetic interactions. Here, we review our studies in which late fetal rat cortex was transplanted heterotopically into the cortex of newborn rats to test its capacity to differentiate features normally unique to other cortical areas. We find that regions of the developing neocortex have similar potentials to differentiate the connectivity and functional architecture that distinguish neocortical areas in the adult. We conclude that the neocortical neuroepithelium generates comparable populations of cells across its extent, and when exposed to the same extrinsic cues, these populations can differentiate in comparable ways. These studies support the concept that the neocortical neuroepithelium generates a "protocortex" (20), specified to have fundamental cortical features but lacking a rigid specification of "area-specific" features. 相似文献
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Actions of some anions on electrical properties and mechanical threshold of frog twitch muscle 总被引:9,自引:1,他引:9
1. Using two micro-electrodes in a point-voltage clamp technique, the effects of the lyotropic anions, NO(3) (-), Br(-), I(-), SCN(-), and CH(3)SO(4) (-), and of SO(4) (2-) on the mechanical threshold and electrical properties of frog sartorius muscle were studied.2. In chloride Ringer solution the spike threshold was -59 mV, mechanical threshold -48 mV, and the threshold for delayed rectification of the total current at about 100 msec -52 mV.3. When Cl(-) was replaced by one of the lyotropic anions, the effective resistance determined at -100 mV tended to increase. But, because of the variability of the effective resistance in individual fibres, most lyotropic anions did not cause a statistically significant increase in the effective resistance. Only I(-) and SO(4) (2-) significantly increased the effective resistance.4. Most lyotropic anions had no significant effect on the spike threshold; I(-), at 58 mM, lowered it slightly. Sulphate raised the threshold.5. Tetrodotoxin (0.1 mug/ml.) abolished the spikes, but did not affect the mechanical and delayed rectification thresholds. It was, therefore, used to pre-treat all preparations for determining these thresholds.6. All lyotropic anions lowered the mechanical and the delayed rectification thresholds, the order of effectiveness being approximately SCN(-) > I(-) > NO(3) (-) > CH(3)SO(4) (-) > Br(-). As in Cl(-) Ringer, the two thresholds lay very close together in every case. Sulphate raised slightly both the mechanical and delayed rectification thresholds, again in close parallel.7. This close agreement of the mechanical and delayed rectification thresholds is not caused by movement artifact, because in fibres in which visible contractions were eliminated with hypertonic solutions the delayed rectification thresholds were the same as those in contracting fibres.8. In spite of the close agreement, reasons are given to doubt a direct causal relationship between the mechanical and delayed rectification thresholds.9. Nitrate apparently had little effect on the rate of inactivation of the outward current, or on the relation between steady-state inactivation and membrane potential. 相似文献