全文获取类型
收费全文 | 969篇 |
免费 | 50篇 |
国内免费 | 4篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 44篇 |
妇产科学 | 9篇 |
基础医学 | 140篇 |
口腔科学 | 53篇 |
临床医学 | 78篇 |
内科学 | 197篇 |
皮肤病学 | 23篇 |
神经病学 | 40篇 |
特种医学 | 21篇 |
外国民族医学 | 4篇 |
外科学 | 111篇 |
综合类 | 3篇 |
预防医学 | 98篇 |
眼科学 | 40篇 |
药学 | 92篇 |
中国医学 | 6篇 |
肿瘤学 | 59篇 |
出版年
2023年 | 7篇 |
2022年 | 14篇 |
2021年 | 25篇 |
2020年 | 22篇 |
2019年 | 21篇 |
2018年 | 21篇 |
2017年 | 29篇 |
2016年 | 24篇 |
2015年 | 25篇 |
2014年 | 36篇 |
2013年 | 47篇 |
2012年 | 78篇 |
2011年 | 72篇 |
2010年 | 42篇 |
2009年 | 41篇 |
2008年 | 65篇 |
2007年 | 64篇 |
2006年 | 64篇 |
2005年 | 52篇 |
2004年 | 65篇 |
2003年 | 54篇 |
2002年 | 31篇 |
2001年 | 3篇 |
2000年 | 11篇 |
1999年 | 15篇 |
1998年 | 9篇 |
1997年 | 5篇 |
1996年 | 3篇 |
1994年 | 4篇 |
1992年 | 4篇 |
1989年 | 6篇 |
1988年 | 4篇 |
1987年 | 2篇 |
1986年 | 4篇 |
1985年 | 3篇 |
1984年 | 8篇 |
1982年 | 3篇 |
1981年 | 2篇 |
1979年 | 3篇 |
1976年 | 2篇 |
1974年 | 4篇 |
1972年 | 4篇 |
1971年 | 2篇 |
1970年 | 3篇 |
1969年 | 6篇 |
1967年 | 1篇 |
1966年 | 1篇 |
1929年 | 1篇 |
1928年 | 1篇 |
1927年 | 1篇 |
排序方式: 共有1023条查询结果,搜索用时 15 毫秒
1.
Marcelo Sato‐Sano Solange Pistori Teixeira Juliano Cordova Vargas Otvio Cesar Carvalho Guimares Baiocchi Milvia Maria Simes e Silva Enokihara Elimar Elias Gomes Mariana Dias Batista 《The Journal of dermatology》2019,46(7):618-621
Eosinophilic dermatosis of hematological malignancy is a paraneoplastic skin eruption associated with chronic lymphocytic leukemia and other B‐cell malignancies. It clinically resembles an insect bite reaction and it can precede the symptoms of the hematological malignancy or be related to a more aggressive course. Different treatments have been proposed, but partial response and recurrence are frequent. Herein, we describe a case of eosinophilic dermatosis associated with mantle cell lymphoma with remission after lenalidomide therapy. 相似文献
2.
3.
Background
Due to marginalization, trafficking violence, conflicts with the police and organic and social psychological problems associated with the drug, crack is one of the most devastating drugs currently in use. However, there is evidence that some users manage to stay alive and active while using crack cocaine for many years, despite the numerous adversities and risks involved with this behavior. In this context, the aim of the present study was to identify the strategies and tactics developed by crack users to deal with the risks associated with the culture of use by examining the survival strategies employed by long-term users. 相似文献4.
Surgical versus nonsurgical treatment of ligamentous injuries following dislocations of the elbow joint 总被引:3,自引:0,他引:3
P O Josefsson C F Gentz O Johnell B Wendeberg 《Clinical orthopaedics and related research》1987,(214):165-169
Sixty-two patients older than 16 years of age at the time of injury were reexamined an average of five years (range, 1 to 12 years) after a dislocation of the elbow without concomitant fracture. Thirty-four were treated nonsurgically with closed reduction and immobilization in a plaster cast. Twenty-eight were treated surgically with primary ligament repair followed by immobilization in plaster. Ligament repair was performed medially in all cases and laterally in 17, on the average two days after injury. At follow-up examination, the most common complaint in both groups was limited range of motion, decreased extension being the most common. In no respect were the surgically treated elbows better than those treated nonsurgically. No evidence was found to recommend primary surgical treatment of ligament injuries associated with dislocation of the elbow. 相似文献
5.
Activation of macrophages in an experimental rat model of arthritis induced by Erysipelothrix rhusiopathiae infection.
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Infection of Lewis rats with Erysipelothrix rhusiopathiae represents an experimental model system of acute and chronic arthritis. We studied here the acute inflammatory phase with respect to stimulation of macrophages and lymphocytes. Intragluteal injection of viable E. rhusiopathiae (10(2) to 10(4) bacteria) rapidly induced generalized inflammation, loss of body weight, hind leg arthritis, and systemic macrophage activation within 2 to 3 days. The same symptoms could also be evoked by injection of dead E. rhusiopathiae. Ex vivo, peritoneal macrophages released large amounts of tumor necrosis factor alpha on day 2 and interleukin-1 on day 3, whereas production of prostaglandin E2 was delayed to days 5 to 7 and appeared to counteract tumor necrosis factor alpha synthesis. The inflammatory response and development of arthritis were strongly dependent on T lymphocytes, as evidenced by the following findings: (i) lymphocytes released lymphokines that activated macrophages to enhanced mediator release; (ii) treatment of rats with cyclosporin A reduced infection-induced macrophage activation; (iii) mitogen-stimulated thymocyte proliferation was enhanced, indicating an infection-induced maturation-differentiation process in the thymus; and (iv) in T-cell-deficient nude rats, a higher dose of bacteria was required for infection, the inflammatory response was less severe, and only mild, but not chronic, arthritis developed. Thus, an E. rhusiopathiae-induced inflammation in rats provides a useful tool to characterize activated macrophages and T lymphocytes during the development of acute arthritis and its transition into the chronic form. 相似文献
6.
Prevalence of enterotoxigenic Escherichia coli strains harboring the longus pilus gene in Brazil 总被引:1,自引:0,他引:1
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
The longus type IV pilus gene (lngA) was highly prevalent (32.8%) among Brazilian enterotoxigenic Escherichia coli strains producing both heat-labile and heat-stable enterotoxins and bearing the CFA/I, CS1CS3, or CS6 antigen. Furthermore, lngA was more often found in strains isolated from children with diarrhea than in strains isolated from children without diarrhea. 相似文献
7.
An IFN-beta-albumin fusion protein that displays improved pharmacokinetic and pharmacodynamic properties in nonhuman primates. 总被引:6,自引:0,他引:6
Cynthia Sung Bernardetta Nardelli David W LaFleur Erich Blatter Marta Corcoran Henrik S Olsen Charles E Birse Oxana K Pickeral Junli Zhang Devanshi Shah Gordon Moody Solange Gentz Lisa Beebe Paul A Moore 《Journal of interferon & cytokine research》2003,23(1):25-36
The long half-life and stability of human serum albumin (HSA) make it an attractive candidate for fusion to short-lived therapeutic proteins. Albuferon (Human Genome Sciences [HGS], Inc., Rockville, MD) beta is a novel recombinant protein derived from a gene fusion of interferon-beta (IFN-beta ) and HSA. In vitro, Albuferon beta displays antiviral and antiproliferative activities and triggers the IFN-stimulated response element (ISRE) signal transduction pathway. Array analysis of 5694 independent genes in Daudi-treated cells revealed that Albuferon beta and IFN-beta induce the expression of an identical set of 30 genes, including 9 previously not identified. In rhesus monkeys administered a dose of 50 microg/kg intravenously (i.v.) or subcutaneously (s.c.) or 300 microg/kg s.c., Albuferon beta demonstrated favorable pharmacokinetic properties. Subcutaneous bioavailability was 87%, plasma clearance at 4.7-5.7 ml/h/kg was approximately 140-fold lower than that of IFN-beta, and the terminal half-life was 36-40 h compared with 8 h for IFN-beta. Importantly, Albuferon beta induced sustained increases in serum neopterin levels and 2',5' mRNA expression. At a molar dose equivalent to one-half the dose of IFN-beta, Albuferon beta elicited comparable neopterin responses and significantly higher 2',5'-OAS mRNA levels in rhesus monkeys. The enhanced in vivo pharmacologic properties of IFN-beta when fused to serum albumin suggest a clinical opportunity for improved IFN-beta therapy. 相似文献
8.
Recombinant soluble tumor necrosis factor receptor proteins protect mice from lipopolysaccharide-induced lethality. 总被引:9,自引:0,他引:9
W Lesslauer H Tabuchi R Gentz M Brockhaus E J Schlaeger G Grau P F Piguet P Pointaire P Vassalli H Loetscher 《European journal of immunology》1991,21(11):2883-2886
The in vivo efficacy of human recombinant soluble tumor necrosis factor (TNF) receptor protein to prevent and to treat lipopolysaccharide (LPS)-induced lethal toxicity in D-galactosamine-treated mice was investigated. Chimeric proteins of the receptor extracellular domains fused to the hinge region of human IgG3 were expressed in myeloma cells (rsTNFR-h gamma 3). The fusion proteins had a disulfide-bonded dimeric structure. Upon intravenous injection, their serum concentration decreased relatively slowly after an initial phase of rapid elimination. D-galactosamine-sensitized mice were fully protected from the toxic effects of LPS, if the animal were pretreated with rsTNFR-h gamma 3 at 20 micrograms/animal. Partial protection was seen at significantly lower doses and when rsTNFR-h gamma 3 was given up to 3 h after LPS. 相似文献
9.
Francesco Novelli Mirella Giovarelli Reiner Gentz Mario Zucca Francesco Di Pierro Gianni Garotta Guido Forni 《European journal of immunology》1993,23(6):1226-1231
Previous work has shown that neutralization of physiologically secreted interferon(IFN)-γ or blockade of its receptor during T lymphocyte activation inhibits both proliferation and cytotoxic T lymphocyte generation, suggesting that IFN-γ plays a crucial role in T lymphocyte induction and differentiation. In this study, the kinetics of the surface expression of the 90-kDa IFN-γ receptor (IFN-γR) was followed during human mixed lymphocyte reaction (MLR) to alloantigens. IFN-γR mRNA is constitutively expressed on resting peripheral blood lymphocytes emerging from nylon wood column (NW-PBL) and its expression increases two- to threefold on alloactivated NW-PBL. IFN-γR protein is poorly expressed on the membrane of resting CD3+ cells, but up-modulates after 3-day MLR and sharply down-modulates at day 6. Both the p55 and the p75 chains of interleukin-2 receptor (IL-2R) were shown to up-modulate in parallel with IFN-γR, whereas they were still highly expressed at day 6. After alloactivation, IFN-γ and IL-2 secretion starts at 24 h, peaks at day 3 and decreases just when IFN-γR and IL-2R begin to up-modulate. Proliferation peaks at day 6. Lastly, stimulation with distinct cell populations showed that the intensity of lymphocyte proliferation, IFN-γR membrane up-modulation, and IFN-γ and IL-2 secretion are regulated in a parallel manner, thus suggesting that they are interrelated. Taken as whole these results demonstrate that increased expression of IFN-γR on T lymphocytes can be a critical event during their activation, and strongly support the hypothesis that IFN-γ/IFN-γR interaction provides a signal for its progression. 相似文献
10.
Amadio Eliane Martins Marcos Rodrigo Labat Serra Andrey Jorge dos Santos Solange Almeida Caires Jheniphe Rocha Fernandes Guilherme Henrique Cardosos Leal-Junior Ernesto Cesar Ferrari João Carlos Correa de Tarso Camillo de Carvalho Paulo 《Lasers in medical science》2021,36(7):1427-1435
Lasers in Medical Science - Photobiomodulation therapy (PBMT) has been indicated for enforcement on healing skin wounds. This study evaluated the effects of PBMT on the healing of skin wounds... 相似文献