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Marupudi Sivaparvathi Ian McCutcheon Raymond Sawaya Garth L. Nicolson Jasti S. Rao 《Clinical & experimental metastasis》1996,14(4):344-350
Increased levels of human cysteine proteases have been implicated in the progression of tumors from the premalignant to the malignant state. The physiological activities of these proteases are regulated by their interactions with specific inhibitors. To our knowledge there have been no previous reports about the cysteine protease inhibitors (CPIs) in human brain tumors. In the study reported here, we determined CPI activity during glioma progression and compared that with normal human brain tissue. We also determined CPI activities in meningioma and glioblastoma cell lines in vitro. This activity was significantly higher in normal brain tissue and low-grade glioma than in anaplastic astrocytoma and glioblastoma. CPI activity was significantly higher in benign and atypical meningioma cell extracts in comparison with those from malignant meningiomas and with those from glioblastoma cell lines. After several passages, one benign meningioma cell line showed reduced levels of CPI and increased levels of cathepsin. Our results suggest that decreases in the activities of CPI may contribute to the malignant properties of brain tumors. 相似文献
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Sivaparvathi Marupudi Yamamoto Masaaki Nicolson Garth L. Gokaslan Ziya L. Fullert Gregory N. Liotta Lance A. Sawaya Raymond Rao Jasti S. 《Clinical & experimental metastasis》1996,14(1):27-34
Recent studies suggest that cysteine proteinase cathepsin L is involved in the process of tumor invasion and metastasis. We
examined cathepsin L activity in brain tumor tissue samples by an enzymatic assay, and cathepsin L protein content by enzyme-linked
immunoadsorbent assays and Western blotting to determine whether increased levels of cathepsin L correlate with the progression
of human gliomas. Native and acid-activatable cathepsin L activities were highest in glioblastomas followed by anaplastic
astrocytomas and were lowest in low-grade gliomas and normal brain tissues. Significantly higher amounts of an M
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29 000 cathepsin L were present in glioblastomas and anaplastic astrocytomas than in normal brain tissues and low-grade glioma
tissue extracts. Using specific antibodies to cathepsin L, we also studied its cellular distribution by immunohistochemical
procedures. Higher diffuse cathepsin L immunoreactivity was found in glioblastomas than in low-grade gliomas and normal brain
tissue samples. Finally, the addition of cathepsin L antibody inhibits the invasion of glioblastoma cell lines through Matrigel
invasion assay. These results suggest the expression of cathepsin L is dramatically upregulated in malignant gliomas and correlates
with the malignant progression of human gliomas in vivo. 相似文献
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