雷公藤总甙(TⅡ)中雷藤氯内酯醇的高效液相色谱分析

建立了雷公藤总甙(T_1)中雷藤氯内酯醇的高效液相色谱分析方法。采用μ-Bondapak C_(18)柱和46％甲醇-水流动相,紫外检测波长219nm。考察了雷藤氯内酯醇的稳定性,雷藤氯内酯醇在0.5～10.0μg/ml范围内线性良好,回收率在98％～101％之间。     

RP-HPLC法测定斑蝥素脂肪乳剂中斑蝥素的含量

目的　首次建立RP HPLC法测定斑蝥素脂肪乳剂中斑蝥素的含量。方法　采用C18柱 ,甲醇 水系统为流动相 ,梯度洗脱 ,紫外检测器 (检测波长为 2 2 7nm ,灵敏度为 0 0 0 5AUFS) ,对斑蝥素脂肪乳剂中斑蝥素进行含量测定。结果　样品处理简单 ,且能将斑蝥素与脂肪乳中的油脂、磷脂等主要杂质分离出来 ,斑蝥素在 2 0 8～ 31 2 μg·ml-1范围内峰面积与浓度线性关系良好 (r=0 994 ) ,最低可定量浓度为 1 0 4 μg·ml-1,样品回收率为 94 8%以上 ,进样精密度为 1 0 2 % (n =5 )。结论　该法测定脂肪乳样品分离效果好 ,回收率高 ,可作为斑蝥素脂肪乳剂质量控制方法。     

经后路椎体横断截骨矫正术治疗陈旧性椎体骨折

目的 介绍自行设计的经后路体横断截骨矫正术治疗陈旧性胸腰椎骨折的方法和疗效。方法 后路正中切口,咬除骨折椎一侧或双侧横突,切除一侧椎弓根。凿除骨折椎后凸部分椎体,直视下从椎体腰部横行切断,用ＲＦ钉复位固定,植骨融合,临床应用１５例。结果 术后椎体畸形完全矫正,脊神经功能均有不同程度恢复。     

骨碎补-续断药对对成骨/破骨代谢的双向调控作用及其对Hif1ɑ基因的影响

目的 分析骨碎补-续断药对对成骨/破骨代谢的调控作用并初步探讨其机制。方法 分别制备空白、低剂量、中剂量及高剂量的骨碎补-续断含药血清。采用CCK-8法、ALP染色法和茜素红染色法,分别观察含药血清对MC3T3-E1细胞增殖、成骨和矿化能力的影响;采用CCK-8法和TRAP染色法观察含药血清对RAW264.7细胞增殖和破骨分化能力的影响。采用系统药理学的方法分析药物的可能作用靶点,并结合RT-PCR和Western-blot的方法验证。结果 中剂量和高剂量骨碎补-续断含药血清可促进MC3T3-E1细胞的增殖,抑制RAW264.7细胞的增殖,中剂量组和高剂量组间没有明显差异;同时,中、高剂量含药血清可促进MC3T3-E1细胞的ALP活性和钙化能力,抑制RAW264.7细胞的TRAP活性,中剂量和高剂量间没有显著性差异。骨碎补-续断药对的可能作用靶点涉及HIF1ɑ,RT-PCR和Western-blot结果证实中剂量含药血清可提高MC3T3-1细胞Hif1ɑ基因的mRNA和蛋白水平及RAW264.7细胞的HIF1ɑ蛋白水平。结论 骨碎补-续断药对具有促进成骨代谢,抑制破骨代谢的作用,HIF...     

微创经皮肾镜取石术治疗复杂性输尿管上段结石

目的:探讨微创经皮肾穿刺输尿管镜取石术(minPCNL)治疗复杂性输尿管上段结石的优越性及有效性。方法:对接受minPCNL治疗的126例患者资料进行回顾性分析。其中单发输尿管上段结石95例;并患同侧肾结石21例;双侧输尿管上段结石10例。结果:所有患者均行一期穿刺取石,共行142次手术。其中单通道穿刺取石120例,二通道6例。结石清除率95.2%。肾造瘘管平均留置时间5天,平均住院时间8天。2例需要输血,其中1例minPCNL术中输血,1例术后5天并发大出血而改开放手术输血。1例术后3天并发高热,经引流、抗感染治疗1周后治愈。无其他重大并发症。结论:minPCNL治疗复杂性输尿管上段结石安全、可行、疗效确切,与经尿道输尿管镜取石术及传统开放手术相比,解决了重度肾积水输尿管迂曲或结石下方输尿管炎症狭窄导致经尿道输尿管镜无法到达结石的难题,同时亦避免了开放手术创伤大、并发症多的问题。     

Immunosubunit β5i Knockout Suppresses Neovascularization and Restores Autophagy in Retinal Neovascularization by Targeting ATG5 for Degradation

PurposeTo investigate the functional role of immunoproteasome subunit β5i in pathologic retinal neovascularization (RNV) and its ability to link the immunoproteasome and autophagy.MethodsOxygen-induced retinopathy (OIR) was induced in wild-type (WT) and β5i knockout (KO) mouse pups on a C57BL/6J background. Proteasome catalytic subunit expression and proteasome activity were evaluated by quantitative real-time PCR (qPCR) and proteasome activity. Retinal vascular anatomy and neovascularization were characterized and quantified by retinal vascular flat-mount staining, fluorescence angiography, platelet endothelial cell adhesion molecule (PECAM) immunostaining, and hematoxylin and eosin staining. Correlation factors, including VEGF and ICAM-1, were detected by qPCR. Autophagy was examined by transmission electron microscopy (TEM). Autophagy biomarkers, including LC3, P62, ATG5, and ATG7, were measured by immunostaining and immunoblotting. The protein interaction between β5i and ATG5 was detected by immunoprecipitation.ResultsWe observed that β5i had the greatest effect in WT OIR mice. Fundus fluorescence angiography, retinal flat-mount staining, and PECAM staining revealed that pathologic RNV decreased in β5i KO OIR mice compared with WT OIR mice. Concurrently, TEM, immunostaining, and immunoblotting showed that autophagy was induced in β5i KO OIR mice compared to WT OIR mice through increases in autophagosome and LC3 expression and a decrease in P62. Mechanistically, β5i interacted with ATG5 and promoted its degradation, leading to autophagy inhibition and pathogenic RNV.ConclusionsThis study identifies a functional role for β5i in RNV regulation. β5i deletion ameliorates RNV and restores autophagy by stabilizing ATG5. These results demonstrate the potential of β5i to serve as a bridge linking the immunoproteasome and autophagy.     

甲基强的松龙对大鼠颅脑损伤后一氧化氮合成酶活性的影响

目的观察甲基强的松龙对大鼠颅脑损伤后血、脑组织中NOS含量的影响,并探讨其作用机制。方法将45只SD大鼠随机分为3组:实验组(20只)、对照组(20只)、正常组(5只),均采用骨窗形成后硬膜外打击法造成鼠脑挫裂伤。正常组麻醉后,只行开颅手术,不作头颅打击,治疗组大鼠致伤后即刻腹腔内注射30mg/kg甲基强的松龙,对照组则注射30mg/kg生理盐水。对照组和治疗组大鼠分别在伤后1h、6h、12h、24h断头取脑,对大鼠脑外伤后脑组织中一氧化碳合成酶(NOS)含量进行检测。结果大鼠脑皮质中的NOS活性在伤后1h较正常组显著性升高(P <0.01),6h开始下降,12～24h降至基础水平。甲基强的松龙治疗组在伤后1h(P <0.01)、6h(P <0.05)NOS活性较损伤组显著性降低。结论颅脑损伤后,受损脑组织中NOS活性升高,甲基强的松龙可通过抑制损伤后NOS活性起到保护创伤神经元的作用。     

Influence of Solvent-Dependent Morphology on Molecular Doping and Charge Transport in Conductive Thiophene Polymer

The utility of a solvent is one of the key factors that impacts resultant film morphology. However, the effect of solvent-dependent morphology on the doping process and electrical conductivity has not been adequately elucidated. In this work, we compared the morphology of chloroform- and chlorobenzene-processed thiophene polymer films and investigated how the choice of solvent influences film morphology, doping level, charge transport properties, and thus electrical conductivity. It was found that the film drop-casted from chloroform exhibits better crystallinity than that drop-casted from chlorobenzene. The crystallinity has negligible impact on the doping level but significant impact on charge transport properties. As a result, the chloroform-processed film shows a higher electrical conductivity of up to 408 S cm⁻¹ due to a high carrier mobility related to the continuously crystalline domains in film. This finding indicates that the choice of solvent for preparation of film, which strongly correlated with molecular orientation, is a new strategy to optimize the electrical conductivity of doped polymers.     

烧伤药油治疗中小面积Ⅱ度烧伤43例临床观察

目的：观察烧伤药油治疗中小面积Ⅱ度烧伤的临床疗效。方法：将Ⅱ度烧伤患者103例随机分为对照组57例、治疗组46例。2组均洗必泰喷洒消毒后,去除已破溃的腐皮,对照组涂抹磺胺嘧啶银乳膏,包扎换药,1次/d,治疗组涂抹院内自制药烧伤药油1 mm厚,保持创面润泽,每日涂药4～6次。2组均连续治疗30天后观察创面愈合时间及临床疗效。结果：治愈率治疗组为58.70%,对照组为36.84%,2组比较差异显著（P＜0.05）。总有效率治疗组为95.65%,对照组为80.70%,2组比较差异显著（P＜0.05）;浅Ⅱ度伤创面平均愈合时间治疗组为（9.6±1.97）天,对照组为（12.3±1.49）天,2组比较差异有统计学意义（P＜0.05）;深Ⅱ度伤创面平均愈合时间治疗组为（18.7±1.89）天,对照组为（22.7±1.53）天,2组比较差异有统计学意义（P＜0.05）。结论：烧伤药油治疗中小面积Ⅱ度烧伤临床疗效显著,可加快创面愈合。