排序方式: 共有47条查询结果,搜索用时 317 毫秒
1.
Shchelkanov MIu Kolobukhina LV Moskvina TM Aushev ID Kartoev AA Kelli EI Merkulova LN Grenkova EP Samokhvalov EI Petriaev VG Serobian AG Klimova EA Galkina IV Malyshev NA Aristova VA Slavskiĭ AA Luk'ianova NA Deriabin PG Gromashevskiĭ VL Efremenko VI Onishchenko GG L'vov DK 《Voprosy virusologii》2005,50(5):9-15
The laboratory verified cases of Crimean-Congo hemorrhagic fever (CCHF) in the piedmont steppes of the North Caucasus (Malgobeksky District, Republic of Ingushetia) are first described. The source of the first infection was Ixodidae ticks; three subsequent sources were contacts with the bloody discharges from patients. CCHF virus genome was detected in the blood of the cattle from an epidemic focus and in the pools of the Ixodes ticks Haemaphysalis parva Neum., 1897 and Boophilus annulatus Say, 1821, taken from cattle. The problem of including the piedmont steppes of the North Caucasus into the CCHF nosological area is discussed. 相似文献
2.
Described in the paper are the results of a 15-year monitoring (1986-2001) of HIV patients at the Clinic of the Institute of Immunology, Federal Department "Medbioextreme", Russia's Health Ministry. The gathered experimental data, including the immunologic, biochemical and general-clinical parameters, were comprehensively analyzed. Optimal schemes of complex HIV monitoring were designed to ensure a comparative and dynamic study of different clinical-and-laboratory indices for the purpose of defining the HIV-induced pathogenesis specificity and adequate therapeutic approaches. 相似文献
3.
L'vov DK Iamnikova SS Fediakina IT Aristova VA L'vov DN Lomakina NF Petrova ES Zlobin VI Khasnatinov MA Chepurgina EA Kovtunov AI Dzharkenov AF Sankov MN Leonova GN Maslov DV Shchelkanov MIu Nepoklonov EA Aliper TI 《Voprosy virusologii》2004,49(3):17-24
The research results on ecology and evolution of influenza A viruses, which has been conducted by the Center of Ecology and Evolution of influenza Viruses of Ivanovsky's Institute of Virology, Russian Academy of Medical Sciences, for more than 30 years, are summarized in the paper. A gene pool of influenza A viruses circulating in Russia's territory was defined. Foci of influenza A viruses were detected in natural biocenosis. Issues conditioned by the population interrelations of influenza viruses, i.e. between the populations of wild and home animals and the populations of people, are also under discussion. 相似文献
4.
Deriabin PG Galegov GA Botikov AG Burtseva EI Mishin DV Shchelkanov MIu 《Voprosy virusologii》2011,56(1):21-24
In vitro experiments revealed that Zanamivir substance had high antiviral activity against infection induced by genotypes 2.2 and 2.3.2 of highly virulent influenza A/H5N1 virus in the porcine embryonic renal epithelial (PERE) cell cultures. Zanamivir at used concentrations (1.0 microg/ml or lower) had no cytotoxic properties and was equally highly effective when used for prevention (1 hour prior to cell inoculation), treatment-and-prevention (at the time of cell inoculation), and treatment (2 hours after inoculation). The high antiviral potential of Zanamivir was shown in the experiments dealing with its effect on the ability of infected PERE cells to produce an infectious virus in the first 24 hours after inoculation. The findings suggest that Zanamivir (Relenza) shows promise as an effective antiinfluenza agent. 相似文献
5.
Vasil'ev AV Shchelkanov MIu Dzharkenov AF Aristova VA Galkina IV L'vov DN Morozova TN Kovtunov AI Grenkova EP Zhernovoĭ AV Shatilova VP Slavskiĭ AA Petrenko MS Chirkizov PF Dybal' VD Leont'ev EA Gabbasov FB Odolevskiĭ EA Ibragimov RM Idrisova RZ Sokolova NN Artiukh NP Andreeva NI Bondarev AD Deriabin PG Gromashevskiĭ VL Nepoklonov EA Aliper TI L'vov DK 《Voprosy virusologii》2005,50(6):36-41
Sera sampled from 2,884 farming animals in the Astrakhan region in 2001 to 2004 were investigated by the hemagglutination inhibition test (HIT) in order to indicate specific antibodies to West Nile virus (WNV). HIT-positive samples were investigated by the neutralization test (NT). WNV antibodies were detected in all the examined species of animals: horses (the proportion of positive tests throughout the observation averaged 9.8%; the agreement with NT results was 94.1%), cattle (6,4 and 72.%), camels (5.2 and 41.7%), pigs (3.1 and 75%), and sheep (2.2 and 57.1). Relationships between the environmental features of WNV in different natural zones, the infection rate, and the conditions of keeping farming animals in the Astrakhan region are analyzed. 相似文献
6.
L'vov DK Shchelkanov MIu Deriabin PG Grebennikova TV Prilipov AG Nepoklonov EA Onishchenko GG Vlasov NA Aliper TI Zaberezhnyĭ AD Kireev DE Krasheninnikov OP Kiriukhin ST Burtseva EI Slepushkin AN 《Voprosy virusologii》2006,51(1):11-14
Six strains of influenza AH5N1 virus were isolated, by using PS and MDCK continuous cell lines from poultry and wild birds, which were collected on July 28, 2005 in the samples taken from 5 examines species of wild birds in the Novosibirsk region during the epizootic outbreak with a high mortality. The strains were identified by means of HIT, RT-PCR, and microchip-based techniques. Two strains, A/Grebe/Novosibirsk/29/05 (H5N1) and A/Duck/Novosibirsk/56/05 (H5N1), were deposited to the Russian State Collection of Viruses (Registration Nos. 2372 and 2371, respectively) with the priority date 08.08.2005. Positive results in RT-PCR for influenza A/H5N1 virus detec- tion were obtained in 100% of the samples from dead and sick poultry; 93% from the clinically healthy poultry kept together with sick one; positive results ranged from 0 to 36%. Sequencing established the identity of genetic characteristics of strains isolated for wild birds and poultry as well as their affiliation to high pathogenic avian influenza (HPAI). Phylogenetic analysis revealed a high homology of hemagglutining of West-Siberian strains and strains isoolated from bar-headed gooses (Eulabeia indica) on the Qinghai Lake (Western China) in the 2005 spring. 相似文献
7.
L'vov DK Prilipov AG Shchelkanov MIu Deriabin PG Shilov AA Grebennikova TV Sadykova GK Liapina OV 《Voprosy virusologii》2006,51(2):15-19
The full-size genomes of 2 highly pathogenetic avian influenza (HPAI) virus strains isolated from a wild great-crested grebe (A/Grebe/Novosibirsk/29/05) and a domestic duck (A/Duck/Novosibirsk/56/05) in the tract of the Chany hollow, Barabino forest-steppe (Novosibirsk Region) during the epizootic outbreak in the summer of 2005. The reproductive properties of these strains successively increase in the series of cell lines BHK-2 --> LEH --> Vero-E6 --> MDCK --> PS. A/Grebe/Novosibirsk/29/05 and A/Duck/Novosibirsk/56/05 were shown to be genetically close in all genomic segments to both each other and a group of HPAI/H5N1 A/Qinghai 05 strains isolated from wild birds on the Kukunor Lake in the northwestern province of Tsinkhai, China, in May 2005. All the above strains have the HPAI/H5-specific amino acid sequence of a proteolytic cleavage site (PQGERRRKKRGLF) with Lys-627 in the protein PB2 (which is associated with increased virulence to mammalian cells), Glu-92 in the protein NS1 (that suppresses an antiviral response in the host), Ser-31 in M2 (that is a marker of rimantadine/amantadine sensitivity), 20-member amino acid deletion in the protein NA (positions 49-68) that is a marker of affiliation to the so-called genotype Z and of increased tropism to poultry. 相似文献
8.
L'vov DK Shchelkanov MIu Deriabin PG Fediakina IT Burtseva EI Prilipov AG Kireev DE Usachev EV Aliper TI Zaberezhnyĭ AD Grebennikova TV Galkina IV Slavskiĭ AA Litvin KE Dongur-ool AM Medvedev BA Dokper-ool MD Mongush AA Arapchor MSh Kenden AO Vlasov NA Nepoklonov EA Suarez D 《Voprosy virusologii》2006,51(6):14-18
Virological and molecular genetic studies of the field material collected in the epicenter of epizooty with high mortality rates among the wild birds on the coast of the Ubsu-Nur lake (Republic of Tyva, 51 degrees NL, 93 degrees EL, June 2006) revealed the etiological role of highly pathogenic avian influenza (HPAI) H5N1. Seven HPAI/H5N1 strains were isolated from the tracheal/cloacal swabs of clinically healthy, ill and recently dead great-crested grebes (Podiceps cristatus), cormorants (Phalacrocorax carbo), balt-coots (Fulica atra), and common terns (Sterna hirundo) collected on June 24, 2006, and incorporated to the RF State Collection of Viruses (with the July 3, 2006 priority). Full-length genome nucleotide sequences were incorporated to the GenBank (with the July 23, 2006 priority) (DQ852600-DQ852607). Comparative analysis of molecular genetic characteristics showed their belonging to the Qinghai-Siberian genotype. The strains were sensitive to rimantadine. 相似文献
9.
Ivanova VT Burtseva EI Slepushkin AN Shchelkanov MIu Zagorskaia IuV Shevchenko ES Rakutina RO Cherkasov EG Isaeva EI Oskerko TA Feodoritova EL Maslov DV Gatich RZ 《Voprosy virusologii》2006,51(1):19-23
Studies indicated that the epidemic rise in the incidence of influenza was caused by its virus A (H3N2) circulation in Russia in the 2003-2004 season. The Center of Influenza Ecology and Epidemiology investigated 101 epidemic strains isolated the MDCK culture. Antigenic analysis showed that all viruses A(H3N2) were similar to the reference virus A/Fujian/411/02(H3N2) and only 5 strains slightly differed from the latter. Twelve (14%) strains resistant to rimantadine at a concentration of 0.5 mg/ml were identified. Investigation of paired sera from the patients demonstrated a rise of antibodies to the references of influenza virus A(H3N2) in 68.7% of cases and a less increase in those to influenza viruses A(H1N1) and B. The active circulation of A(H3N2) viruses was due not only to changes in their antigenic structure, but also to the low level of antibodies to these viruses, as shown by the analysis of donor sera. 相似文献
10.
The paper contains the results of a 15-year monitoring (since 1986 to 2001) of HIV-infected patients followed up at the clinic of the Institute of Immunology, Federal Department "Medbioextrem", Russia's Health Ministry. An analysis of a category of HIV-infected persons, who practiced the intravenous consumption of drugs since 1997 to 2001, revealed not only concomitant multi-virus infections (hepatitis C--68%, hepatitis B--48%, herpes 41%, fungal infections--66% and bacterial infections--46%) but also lesions (up to 70%) in the gastrointestinal tract and in the hepatic-cellular and hepatic-biliary systems. It was established that, when only immunological indices are taken account of in presence of multi-virus infections, it does not ensure an adequate evaluation of an HIV-patient condition. The below biochemical parameters must be necessarily taken into consideration: activity of alanine aminotransferase, aspartate aminotransferase and of alkaline phosphatase. The changes in the biochemical parameters were shown to be essentially higher in HIV patients with hepatitis C virus (HCV) even during the 2 initial follow-up years, when the immunological parameters are in the norm or even insignificantly below it. A comparative analysis of dynamic clinical, immunological and biochemical indices was made within the case study for the purpose of defining the prognostic criteria for HIV progression scenarios. 相似文献