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排序方式: 共有286条查询结果,搜索用时 15 毫秒
1.
Evaluation of three substitutes for Percoll in sperm isolation by density gradient centrifugation 总被引:9,自引:4,他引:9
Silane-coated silica particle solutions (ISolate(TM) and PureSperm)TM)) and
iodixanol (OptiPrep(TM)) were compared to polyvinylpyrrolidone (PVP)-coated
silica particles (Percoll(TM)) in their efficacy to recover spermatozoa by
gradient centrifugation for use in assisted reproductive procedures.
Efficacy was assessed in terms of percentages of sperm recovery, sperm
vitality and motility, normal sperm morphology and normal sperm chromatin
condensation. No significant difference was found in the recovery of
spermatozoa for men with both normal sperm counts and oligozoospermia,
between PVP-coated and silane-coated particle solutions. Iodixanol had
significantly lower sperm recovery compared to the other products. Sperm
vitality, progressive motility, normal morphology and normal chromatin
condensation did not differ significantly between any of the sperm
isolation products.
相似文献
2.
Protective cytotoxic T lymphocyte responses against paramyxoviruses induced by epitope-based DNA vaccines: involvement of IFN-gamma 总被引:1,自引:0,他引:1
Hsu SC; Obeid OE; Collins M; Iqbal M; Chargelegue D; Steward MW 《International immunology》1998,10(10):1441-1447
Plasmid DNA vectors have been constructed with minigenes encoding a single
cytotoxic T lymphocyte (CTL) epitope from either the M2 protein of
respiratory syncytial virus (RSV) or from the nucleoprotein of measles
virus (MV) with or without a signal sequence (also called secretory or
leader sequence). Following intradermal immunization, plasmids in which the
CTL epitopes were expressed in-frame with the signal sequence were more
effective at inducing peptide- and virus- specific CTL responses than
plasmids expressing CTL epitopes without the signal sequence. This
immunization resulted in protection against MV-induced encephalitis and a
significant reduction in viral load following RSV challenge. The reduction
of viral load following RSV challenge was abrogated by prior injection with
anti-IFN-gamma antibodies. These results highlight the ability of
epitope-based DNA immunization to induce protective immune responses to
well-defined epitopes and indicate the potential of this approach for the
development of vaccines against infectious diseases.
相似文献
3.
S. R. Handrakala ulian A. Crasta hameem Shariff 《Indian journal of otolaryngology and head and neck surgery》2003,55(4):275-277
Sialomrgaly of suhmandibultir gland, whether inflammatory or neoplastic can be clinically mistaken for lymphoid metastasis
especially when encountered in an elderly patient. Other than the neoptastic lesions and usual inflammatory lesions rarely
amylase crystalloids can evoke a granulomatous response and can result in sialomegaly. We report an interesting case which
presented as a submandibular swelling and was clinically diagnosed as metastasis where the diagnosis of granulomatous response
to crystalloids was made at FNAC. The aim of this report is to bring an awareness of the existence of such lesions and to
highlight the role of FNAC in diagnosis of such lesions in order to avoid unnecessary surgeries. 相似文献
4.
M. Ismail Shareef S. Leelavathi Anis Ahmed Shariff 《Proceedings of the National Academy of Sciences, India. Section B.》2013,83(2):187-191
In-vivo studies have suggested the anti-rheumatic and immunomodulatory properties of the methanolic extract of the plant Anisomeles malabarica R.Br. However, the mechanism of action of this plant in the modulation of inflammation using the various in vitro models has not been explored earlier. Hence, the current investigation was undertaken to study the anti-rheumatic and immunomodulatory role of aerial parts, leaves and roots in lipopolysaccharide (LPS) mediated signaling in macrophage and mouse connective tissue cell cultures. It was observed from the present study that by employing tumor necrosis factor-α (TNF-α) bioassay, all the three extracts viz., aerial parts, leaves and roots inhibited TNF-α production in LPS (1 μg/mL) stimulated RAW-32 cells. 38.75 % inhibition of TNF-α was observed at 200 μg extracts of the aerial parts of the plant followed by 17.64 and 14.94 % by the roots and leaves respectively. Taken together, these findings from the present in vitro studies suggest the anti-rheumatic and immunomodulatory properties of the methanolic extracts of A. malabarica. 相似文献
5.
Shaik Mohamed Asif Shaik Mohamed Shamsudeen Khalil Ibrahim Assiri Hussain Mohammed Al Muburak Sultan Mohammed Kaleem Abdul Ahad Khan Mansoor Shariff 《Medicine》2021,100(17)
Introduction:Drug induced oral erythema multiforme a rare clinical entity which involves only the lips and oral mucosa without skin involvement. These lesions are difficult in diagnosing with other oral ulcerative lesions with similar clinical manifestations.Patient concerns:This article presents 2 case reports of Oral erythema multiforme in which drugs were the precipitating factor. Its etiopathogenesis, differential diagnosis and treatment modalities of the disease is discussed.Diagnosis:Based on patient''s complaints, drug history and clinical appearance, provisional diagnosis of drug induced erythema multiforme was considered.Intervention:For case 1, patient was instructed to discontinue usage of drug and prescribed systemic steroid (Prednisolone 10 mg/d) for a week along with germicidal drugs to prevent secondary infection. Medication was tapered to 5 mg/d after first week.For case 2, patient was instructed to discontinue the drug and systemic steroid prednisolone 20 mg /d for 1 week with tapering dose of 10 mg/d for the second week was administered.Outcome:For case 1 and case 2 healing of the lesions were evident on third week of follow up.Conclusion:Medications should be taken under medical supervision. Over the counter drugs might lead to allergic reactions like drug induced oral erythema multiforme, which is a rare variant and needs to be differentiate from other oral ulcerative lesion for prompt management and follow-up. 相似文献
6.
Fara Wahida Rezali Yit Siew Chin Zalilah Mohd Shariff Barakatun Nisak Mohd Yusof Kaartina Sanker Fui Chee Woon 《Nutrition Research And Practice》2015,9(5):511-516
BACKGROUND/OBJECTIVES
This study aims to determine contribution of meal frequency, self-efficacy for healthy eating, and availability of healthy foods towards diet quality of adolescents in Kuala Lumpur, Malaysia.SUBJECTS/METHODS
This study was conducted among 373 adolescents aged from 13 to 16 years old. Diet quality of the respondents was assessed using the Healthy Eating Index for Malaysians. Meal frequency, self-efficacy for healthy eating, and availability of healthy foods were assessed through the Eating Behaviours Questionnaire (EBQ), self-efficacy for healthy eating scale, and availability of healthy foods scale, respectively.RESULTS
The majority of the respondents (80.7%) were at risk of poor diet quality. Males (mean = 34.2 ± 8.2%) had poorer diet quality than females (mean = 39.9 ± 9.0%) (t = -5.941, P < 0.05). Malay respondents (mean = 36.9 ± 8.7%) had poorer diet quality than Indian respondents (mean = 41.3 ± 10.0%) (F = 2.762, P < 0.05). Age (r = 0.123, P < 0.05), self-efficacy for healthy eating (r = 0.129, P < 0.05), and availability of healthy foods (r = 0.159, P < 0.05) were positively correlated with the diet quality of the respondents. However, meal frequency was not correlated with the diet quality of the respondents. Multiple linear regression analysis showed that being a male, being a Malay, low self-efficacy for healthy eating, and low availability of healthy foods contributed significantly towards poor diet quality among respondents.CONCLUSIONS
In short, sex, ethnicity, self-efficacy for healthy eating, and availability of healthy foods were associated with diet quality among adolescents. Health practitioners should take into consideration of differences in sex and ethnicity during implementation of nutrition-related intervention programs. Self-efficacy for healthy eating and availability of healthy foods should be included as important components in improving diet quality of adolescents. 相似文献7.
Abou-Al-Shaar Hussam Mallela Arka N. Patel Aneek Shariff Rimsha K. Shin Samuel S. Choi Phillip A. Faraji Amir H. Fazeli Pouneh K. Costacou Tina Wang Eric W. Fernandez-Miranda Juan C. Snyderman Carl H. Gardner Paul A. Zenonos Georgios A. 《Pituitary》2022,25(3):508-519
Pituitary - To review our institutional experience with the surgical management of prolactinomas through the endoscopic endonasal approach with specific focus on cavernous sinus invasion.... 相似文献
8.
9.
Variations in the morphology of pancreas are not very common. We observed a rare variant of the uncinate process of the pancreas that extended in the mesentery of the small intestine. After its origin from the lower part of the head of the pancreas, the mesenteric process (MP) passed over the third part of the duodenum to enter the mesentery of the jejunum and ileum and extended up to the level of the pelvic brim. The branches of the superior mesenteric vessels were embedded in the extended MP of the pancreas. This aberrant extension of the pancreas was drained by a narrow duct, which joined the main pancreatic duct inside the head. We did not observe a separate uncinate process arising from the head. On histology normal acini and endocrine cells were observed in the extension. This variant is important as symptoms of pancreatic disease from such extensions may be confused with other commonly encountered acute or chronic abdominal conditions. 相似文献