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1.
Selene Dall' Acqua Coutinho José Daniel Fedullo Sandra Helena Corrêa 《Medical mycology》2006,44(4):383-387
The objective of this study was to determine the presence of different species of the genus Malassezia in the healthy external auditory canal of wild felids maintained in captivity. One hundred and thirty-two adult animals (264 samples of cerumen), 77 males (58.3%) and 55 females (41.7%), were studied: large felids (55 animals) - 26 lions (Panthera leo), 13 tigers (Panthera tigris), 6 leopards (Panthera pardus), 6 jaguars (Panthera onca), 2 cheetahs (Acinonyx jubatus), 2 pumas (Puma concolor); small felids (77 animals) - 29 tiger cats (Leopardus tigrinus), 19 jaguarundis (Herpailurus yagouaroundi), 10 margays (Leopardus wiedii), 9 pampas cats (Oncifelis colocolo), 6 geoffroy's cats (Oncifelis geoffroyi), and 4 servals (Leptailurus serval). Samples were obtained by the introduction of a sterile swab into the ear canal after cleaning the auricle with an alcohol-ether solution. The swabs were seeded onto Petri dishes containing modified Mycosel agar and sterile olive oil was added to the surface of the medium before specimen seeding. The plates were incubated at 35oC for two weeks. The isolates were analyzed regarding macro-and micromorphology and identified through catalase tests and growth on Tween 20, 40, 60 and 80. Malassezia spp. were isolated from 58 of the felids studied (43.9%) and from 102 samples of cerumen (38.6%). Malassezia sympodialis was isolated exclusively in large felids (33 animals-56.9%), and Malassezia pachydermatis exclusively in smaller varieties (25 animals - 43.1%). The incidence of fungi was higher in lions, with yeast being isolated in 25 of 26 animals (96.2%). Forty-eight strains (47.1%) were isolated from the right ear canal and 54 (52.9%) from the left. Although M. pachydermatis is the species considered a member of the microbiota of the mammalian external ear canal these results suggest that M. sympodialis participates in the microbiota of large felids. 相似文献
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Nerea Matamala Beatriz Lara Gema Gómez-Mariano Selene Martínez Irene Vázquez-Domínguez Álvaro Otero-Sobrino Antonio Muñoz-Callejas Elena Sánchez Cristina Esquinas Ana Bustamante Sergio Cadenas Sergio Curi Lourdes Lázaro María Teresa Martínez Esther Rodríguez Marc Miravitlles María Torres-Duran Inés Herrero Beatriz Martínez-Delgado 《Archivos de bronconeumologia》2021,57(7):457-463
IntroductionAlpha-1 antitrypsin deficiency (AATD) is a genetic condition resulting in lung and liver disease with a great clinical variability. MicroRNAs have been identified as disease modifiers; therefore miRNA deregulation could play an important role in disease heterogeneity. Members of miR-320 family are involved in regulating of multiple processes including inflammation, and have potential specific binding sites in the 3′UTR region of SERPINA1 gene. In this study we explore the involvement of miR-320c, a member of this family, in this disease.MethodsFirstly in vitro studies were carried out to demonstrate regulation of SERPINA1 gene by miR-320. Furthermore, the expression of miR-320c was analyzed in the blood of 98 individuals with different AAT serum levels by using quantitative PCR and expression was correlated to clinical parameters of the patients. Finally, HL60 cells were used to analyze induction of miR-320c in inflammatory conditions.ResultsOverexpression of miR-320 members in human HepG2 cells led to inhibition of SERPINA1 expression. Analysis of miR-320c expression in patient's samples revealed significantly increased expression of miR-320c in individuals with pulmonary disease. Additionally, HL60 cells treated with the pro-inflammatory factor lipopolysaccharide (LPS) showed increase in miR-320c expression, suggesting that miR-320c responds to inflammation.ConclusionOur findings demonstrate that miR-320c inhibits SERPINA1 expression in a hepatic cell line and its levels in blood are associated with lung disease in a cohort of patients with different AAT serum levels. These results suggest that miR-320c can play a role in AAT regulation and could be a biomarker of inflammatory processes in pulmonary diseases. 相似文献
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Selene Cansino Frine Torres-Trejo Cinthya Estrada-Manilla Joyce Graciela Martínez-Galindo Evelia Hernández-Ramos Mariana Ayala-Hernández Tania Gómez-Fernández María Dolores Ramírez-González Silvia Ruiz-Velasco 《Age (Dordrecht, Netherlands)》2018,40(3):293-303
Working memory abilities significantly decrease with advancing age; hence, the search for factors that may increase or mitigate this decline is critical. Several factors have been identified that influence working memory; however, their effects have been mainly assessed separately and rarely together with other factors in the same sample. We examined 120 variables to search for factors that jointly act as mediators of working memory decay across the adult life span. A sample of 1652 healthy adults was assessed in spatial and verbal working memory domains. Structural equation modeling analyses were conducted to search for potential mediators that intervened between age and working memory. Only 14 and 10 variables reliably mediated spatial and verbal working memory, respectively. Factors from several domains remained in the models, such as individual characteristics, physiological traits, consumption habits, and regular activities. These factors are sufficiently powerful to influence working memory decline when they jointly interact, as in everyday living. 相似文献
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Adriano N. Kochi MD MSc Massimo Moltrasio MD Fabrizio Tundo MD PhD Stefania Riva MD Ciro Ascione MD Maria A. Dessanai MD Francesca Pizzamiglio MD Giulia Vettor MD Selene Cellucci CCP Alessio Gasperetti MD Claudio Tondo MD PhD Gaetano Fassini MD 《Journal of cardiovascular electrophysiology》2021,32(3):588-594
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Glauber Pacelli Gomes de Lima Terezinha Maria de Souza Gabrielle de Paula Freire Davi Felipe Farias Arcelina Pacheco Cunha Nágila Maria Pontes Silva Ricardo Selene Maia de Morais Ana Fontenele Urano Carvalho 《Parasitology research》2013,112(5):1953-1958
This study assessed new insecticidal activities of essential oils from Lippia sidoides and Croton species (Croton zehntneri, Croton nepetaefolius, Croton argyrophylloides, and Croton sonderianus) against Aedes aegypti mosquito. In addition, the acute toxicity upon mice was determined. All essential oils showed inhibition of egg hatching, with IC50 values ranging from 66.4 to 143.2 μg?mL?1, larvicidal activity with LC50 ranging from 25.5 to 94.6 μg?mL?1, and pupicidal action with PC50 ranging from 276.8 to over 500 μg?mL?1. Only L. sidoides, C. zehntneri, and C. argyrophylloides essential oils were able to inhibit the oviposition of female gravid mosquitoes with OD50 values of 35.3, 45.3, and 45.8 μg?mL?1, respectively. Oral acute toxicity in mice showed that C. sonderianus and C. argyrophylloides oils are nontoxic (LD50?>?6,000 mg.kg?1) while C. nepetaefolius, C. zehntneri, and L. sidoides oils are moderately toxic (LD50 3,840; 3,464, and 2,624 mg.kg?1, respectively). The results indicate that these oils are promising sources of bioactive compounds, showing low or no toxicity to mammals. 相似文献
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Bennardo Francesco Liborio Francesco Barone Selene Antonelli Alessandro Buffone Caterina Fortunato Leonzio Giudice Amerigo 《Clinical oral investigations》2021,25(6):3747-3755
Clinical Oral Investigations - Oral lichen planus (OLP) is a chronic immune-mediated disease that affects the oral cavity. Topical steroids are considered the treatment of choice for painful... 相似文献