全文获取类型
收费全文 | 273篇 |
免费 | 17篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 5篇 |
妇产科学 | 2篇 |
基础医学 | 21篇 |
口腔科学 | 53篇 |
临床医学 | 29篇 |
内科学 | 64篇 |
皮肤病学 | 5篇 |
神经病学 | 17篇 |
特种医学 | 11篇 |
外科学 | 43篇 |
综合类 | 1篇 |
预防医学 | 9篇 |
药学 | 23篇 |
中国医学 | 3篇 |
肿瘤学 | 2篇 |
出版年
2023年 | 1篇 |
2022年 | 7篇 |
2021年 | 20篇 |
2020年 | 10篇 |
2019年 | 20篇 |
2018年 | 6篇 |
2017年 | 5篇 |
2016年 | 5篇 |
2015年 | 8篇 |
2014年 | 12篇 |
2013年 | 17篇 |
2012年 | 18篇 |
2011年 | 16篇 |
2010年 | 6篇 |
2009年 | 5篇 |
2008年 | 19篇 |
2007年 | 17篇 |
2006年 | 19篇 |
2005年 | 22篇 |
2004年 | 17篇 |
2003年 | 9篇 |
2002年 | 7篇 |
2001年 | 1篇 |
1999年 | 1篇 |
1996年 | 1篇 |
1995年 | 2篇 |
1994年 | 1篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1983年 | 1篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1979年 | 3篇 |
1978年 | 1篇 |
1977年 | 1篇 |
1974年 | 1篇 |
1972年 | 1篇 |
1971年 | 1篇 |
1967年 | 1篇 |
1964年 | 3篇 |
排序方式: 共有290条查询结果,搜索用时 31 毫秒
1.
Floriano P Silva-Junior Herbert L M Guedes Laura C Garvey Aniesse S Aguiar Saulo C Bourguignon Enrico Di Cera Salvatore Giovanni-De-Simone 《Toxicon》2007,50(1):18-31
BJ-48, a serine protease from the venom of Bothrops jararacussu, was purified to homogeneity using affinity chromatography on p-aminobenzamidine-agarose followed by HPLC gel filtration. BJ-48 presented 52kDa by SDS-PAGE analysis and 48,036Da by electron spray mass spectrometry. The enzyme was shown to be highly glycosylated with 42% of N-linked carbohydrates composed of Fuc(1):GalN(4):GlcN(5):Gal(1):Man(2) and a high content of sialic acid residues (8-12%). BJ-48 had optimal esterase activity at pH 7.5 and displayed maximum catalytic rate at 50 degrees C. Its hydrolytic activity was strongly inhibited by aprotinin and dithiothreitol while N-tosyl-l-phenylalanine chloromethyl ketone, 6-aminocaproic acid, E-64 and soybean trypsin inhibitor (SBTI) were ineffective. The kinetics of BJ-48 with chromogenic substrates revealed an unprecedented selectivity (10(4)-fold) for Arg over Lys in P1. BJ-48 proved to be a thrombin-like enzyme (TLE) with a specific fibrinogen-clotting activity of 73.4NIH units/mg. The TLE rapidly digested human fibrinogen Bbeta chain, but the Aalpha chain was cleaved specifically to release fibrinopeptide A with k(cat)/K(m)=2.1muM(-1)s(-1). The TLE showed no activity toward other thrombin substrates like protein C, protease-activated receptor-1 or inhibitors such as hirudin and antithrombin. A non-denaturing procedure using PNGase F and neuraminidase followed by hydrophobic interaction chromatography was employed to obtain active BJ-48 forms with variable carbohydrate content. Compared to the native enzyme, total or partially deglycosylated BJ-48 forms presented up to 2-fold reduction in their specific activities upon heating at 55/65 degrees C or treatment with SBTI. These results point out a role for BJ-48 glycosylation in thermostability and controlling the access of some canonical protein inhibitors to the active site. 相似文献
2.
3.
Fava GA Ruini C Rafanelli C Finos L Salmaso L Mangelli L Sirigatti S 《Psychotherapy and psychosomatics》2005,74(1):26-30
BACKGROUND: There is increasing awareness that the goal of treatment in generalized anxiety disorder (GAD) should not simply be a response, but restoration of normal function. The aim of this study was to apply a novel psychotherapeutic approach for increasing the level of remission in GAD. METHODS: Twenty patients with DSM-IV GAD devoid of comorbid conditions were randomly assigned to 8 sessions of cognitive behavioral therapy (CBT) or the sequential administration of 4 sessions of CBT followed by other 4 sessions of well-being therapy (WBT). Assessment methods included the Anxiety and Depression Scales of Paykel's Clinical Interview for Depression, Ryff's Psychological Well-being Scales and Kellner's Symptom Questionnaire. A one-year follow-up was undertaken. RESULTS: Significant advantages of the CBT-WBT sequential combination over CBT only were observed with both observer and self-rated methods after treatment. Such gains were maintained at follow-up. CONCLUSIONS: These preliminary results suggest the feasibility and clinical advantages of adding WBT to the treatment of GAD. They lend support to a sequential use of treatment components for achieving a more sustained recovery. 相似文献
4.
Humberto Oliart-Guzmán Breno M. Delfino Antonio C. Martins Saulo A. S. Mantovani Athos M. Bra?a Thasciany M. Pereira Fernando L. C. C. Branco Alanderson A. Ramalho Rhanderson G. Campos Pablo S. Fontoura Thiago S. de Araujo Cristieli S. M. de Oliveira Pascoal T. Muniz Guita Rubinsky-Elefant Cláudia T. Code?o M?nica da Silva-Nunes 《The American journal of tropical medicine and hygiene》2014,90(4):670-681
Toxocara spp. infection and the seroconversion rate in the Amazon have been poorly investigated. This study analyzed individual and household-level risk factors for the presence of IgG antibodies to Toxocara spp. in urban Amazonian children over a period of 7 years and evaluated the seroconversion rates over a 1-year follow-up. In children < 59 months of age, the overall prevalence rate was 28.08% in 2003 and 23.35% in 2010. The 2010–2011 seroconversion rates were 13.90% for children 6–59 months of age and 12.30% for children 84–143 months of age. Multilevel logistic regression analysis identified child age, previous wheezing, and current infection with hookworm as significant associated factors for Toxocara spp. seropositivity in 2003. In 2010, age, previous helminthiasis, and having a dog were associated with seropositivity, whereas having piped water inside the household was a protective factor. Control programs mainly need to target at-risk children, water quality control, and animal deworming strategies. 相似文献
5.
Rosário PW Cardoso LD Fagundes TA Barroso AL Padrão EL Rezende LL Purisch S 《Arquivos brasileiros de endocrinologia e metabologia》2004,48(4):480-486
This study analyzed serum thyroglobulin (Tg) during hypothyroidism in 207 patients with differentiated thyroid carcinoma treated with total thyroidectomy and radioiodine ablation and undetectable anti-Tg antibodies. Disease staging was defined by clinical examination, stimulated Tg, pre- and post-ablative radioiodine scanning, and other imaging methods (X-Ray, US, CT and MIBI-scan). The average interval from initial therapy was 2.3 years. 153 patients (74%) had no evident disease, 34 (16.4%) presented neck/mediastinal disease, and 20 (9.6%) had distant metastases (Mt). The best cut-off for Tg was 1 ng/ml, showing 100% sensitivity for distant Mt and 88.2% for local recurrence or lymph node Mt, and 88.8% specificity for any Mt and 74.8% for distant Mt. In patients with Tg <1 ng/ml, 2.8% showed cervical lymph nodes Mt. Cervical or mediastinal disease were 26% of cases with Tg between 1 and 5 ng/ml. Tg from 5 to 10 ng/ml was associated to distant Mt in 14.2% of the cases and others showed lymph nodes Mt. In patients with Tg >10 ng/ml, 51.3% presented distant Mt. We suggest the need for neck US even in cases with Tg <1 ng/ml. In addition, patients with Tg levels <5 ng/ml should be investigated by neck US and mediastinal CT only, and empirical therapy should be limited to patients with a minimum Tg level >5 ng/ml. 相似文献
6.
7.
Anne Caroline Costa Oenning Sergio Lins de Azevedo Vaz Saulo Leonardo Sousa Melo Francisco Haiter‐Neto 《Dental traumatology》2013,29(6):489-493
Horizontal root fractures (HRF) usually affect anterior teeth of male patients as a result of trauma. The consequences can be complex because of combined damage to different tissues, but it has been reported that root fractures undergo healing in majority cases. Diagnosis of HRF must be based on clinical findings, sensibility tests, and radiographic examination. The cone‐beam computed tomography (CBCT) has already been proven to be superior among other radiographic modalities for diagnostic imaging of root fractures. However, CBCT in these cases needs to be used in a careful manner, and only when the radiation exposure should be justified by the potential diagnostic benefits and improvement of the treatment results. This case report describes a case of spontaneously healed horizontal root fracture with displacement of the fragments and discusses the usefulness of CBCT in the follow‐up of root fractures cases. 相似文献
8.
Saulo Maia d’Avila Melo 《Obesity surgery》2013,23(3):372-378
Background
There is an increased prevalence of asthmatic, obese patients undergoing bariatric surgery. The objective of our study is to evaluate the severity, level of control, respiratory medication use, and time required for prebariatric surgery asthma control.Methods
This is a prospective study in which 88 obese asthmatics were evaluated by a pulmonologist in two steps, prebariatric surgery. In the first step, patients were evaluated for severity, level of control, and respiratory medication in use, categorized as bronchodilators and corticosteroids. In the second step, the time required for asthma control between steps and appropriate respiratory medication was determined.Results
Thirty-eight obese patients (43.2%) had intermittent asthma, 22 had mildly persistent (25.0%), 24 moderately persistent (27.3%), and 4 severely persistent (4.5%). There were 43 patients with controlled asthma (48.9%), 31 partly controlled (35.2%), and 14 uncontrolled (15.9%). The study sample showed a significant increase in bronchodilators in the first step and corticosteroids in the second step (p?≤?0.0001). Comparisons between steps showed significant differences with a reduction of bronchodilators and increase in corticosteroids in the second step (p?≤?0.0001). The mean time (days) required for asthma control between steps was 28.98?±?33.40 days, with significant differences between groups (p?≤?0.001).Conclusions
In prebariatric surgery, there was a higher proportion of intermittent asthma and uncontrolled asthma, with asthma severity influencing the achievement of asthma control and the time required for surgical release. 相似文献9.
Saulo J.A. Felizola Yasuhiro Nakamura Fumitoshi Satoh Ryo Morimoto Kumi Kikuchi Tomohiro Nakamura Atsushi Hozawa Lin Wang Yoshiaki Onodera Kazue Ise Keely M. McNamara Sanae Midorikawa Shinichi Suzuki Hironobu Sasano 《Molecular and cellular endocrinology》2014
Background
l-glutamate is a major excitatory neurotransmitter in the mammalian brain. Glutamate receptors have been reported in the rat adrenal cortex and in human aldosterone-producing adenomas (APA). However, details regarding the expression levels and functions of these receptors in human adrenocortical tissues remain unknown.Methods
The mRNA levels of glutamate receptors were evaluated by qPCR in: 12 normal adrenal cortex (NAC), 11 APA, and 12 cortisol-producing adenoma (CPA) tissues. Protein localization was evaluated by immunohistochemistry for 15 NAC, 5 idiopathic hyperaldosteronism cases, 15 APA and 15 CPA. H295R cells were treated with angiotensin-II or forskolin alone or combined with the GRM2/3 agonist LY354740.Results
The level of GRM3 mRNA was higher in APA than in CPA (P = 0.0086) or NAC (P = 0.0022). GRM1, IGLUR2, and IGLUR3 were also detected in adrenocortical tissues. When added to angiotensin-II/forskolin treatments, LY354740 decreased aldosterone and cortisol production in H295R cells.Conclusions
GRM3 is considered to regulate steroidogenesis in adrenocortical tissues. 相似文献10.
Elon F. Freitas Roberto Y. Souza Saulo T. A. Passos Jos A. Dias Silvia C. L. Dias Brenno A. D. Neto 《RSC advances》2019,9(46):27125
Herein, a combination of heteropolyacids and ionic liquids as a catalytic system was studied for the Biginelli multicomponent reaction; the positive ionic liquid effect associated with the acidic strength of zeolite-supported heteropolyacids made this combination an efficient catalytic system for the multicomponent synthesis of 3,4-dihydropyrimidin-2(1H)-one/thione derivatives. The acidic strength effect was evaluated, and a range was determined in which the reaction provided better results. The mechanism of the reaction was also investigated in the presence and absence of ionic liquids, and two features of paramount importance were revealed: the mechanism could be tuned to proceed through only one reaction path among three possibilities and the kinetics of the reaction was significantly faster in the presence of an ionic liquid.Heteropolyacids and ionic liquid effect allowed tuning of the Biginelli reaction mechanism and synthesis of 3,4-dihydropyrimidin-2(1H)-one/thione derivatives in an efficient, recyclable fashion. The role of acidic strength and supported heteropolyacid is disclosed. 相似文献