全文获取类型
收费全文 | 1537篇 |
免费 | 57篇 |
国内免费 | 5篇 |
专业分类
耳鼻咽喉 | 11篇 |
儿科学 | 48篇 |
妇产科学 | 4篇 |
基础医学 | 213篇 |
口腔科学 | 24篇 |
临床医学 | 77篇 |
内科学 | 274篇 |
皮肤病学 | 14篇 |
神经病学 | 62篇 |
特种医学 | 117篇 |
外科学 | 248篇 |
综合类 | 127篇 |
一般理论 | 3篇 |
预防医学 | 65篇 |
眼科学 | 27篇 |
药学 | 80篇 |
中国医学 | 9篇 |
肿瘤学 | 196篇 |
出版年
2022年 | 11篇 |
2021年 | 43篇 |
2020年 | 12篇 |
2019年 | 14篇 |
2018年 | 25篇 |
2017年 | 15篇 |
2016年 | 22篇 |
2015年 | 22篇 |
2014年 | 30篇 |
2013年 | 31篇 |
2012年 | 50篇 |
2011年 | 42篇 |
2010年 | 49篇 |
2009年 | 43篇 |
2008年 | 52篇 |
2007年 | 50篇 |
2006年 | 71篇 |
2005年 | 49篇 |
2004年 | 59篇 |
2003年 | 55篇 |
2002年 | 50篇 |
2001年 | 44篇 |
2000年 | 41篇 |
1999年 | 52篇 |
1998年 | 44篇 |
1997年 | 33篇 |
1996年 | 39篇 |
1995年 | 30篇 |
1994年 | 43篇 |
1993年 | 20篇 |
1992年 | 35篇 |
1991年 | 32篇 |
1990年 | 34篇 |
1989年 | 43篇 |
1988年 | 35篇 |
1987年 | 44篇 |
1986年 | 30篇 |
1985年 | 30篇 |
1984年 | 21篇 |
1983年 | 20篇 |
1982年 | 14篇 |
1981年 | 12篇 |
1980年 | 12篇 |
1979年 | 10篇 |
1978年 | 8篇 |
1977年 | 10篇 |
1975年 | 8篇 |
1969年 | 9篇 |
1968年 | 7篇 |
1967年 | 9篇 |
排序方式: 共有1599条查询结果,搜索用时 15 毫秒
1.
2.
3.
4.
Shigeji Matsumoto Mizuho Ikeda Toshimi Nishikawa Shinki Yoshida Takeshi Tanimoto Masahiro Ito Chikako Saiki Mamoru Takeda 《The Journal of pharmacology and experimental therapeutics》2002,300(2):597-604
The excitatory responses of deflationary slowly adapting pulmonary stretch receptor (SAR) activity to lung deflation ranging from approximately -15 to -25 cm of H(2)O for approximately 5 s were examined before and after administration of flecainide, a Na(+) channel blocker, and K(+) channel blockers, such as 4-aminopyridine (4-AP) and tetraethylammonium (TEA). The experiments were performed in anesthetized, artificially ventilated rats after unilateral vagotomy. The deflationary SARs increased their activity during lung deflation and its effect became more pronounced by increasing the degree of negative pressure. During lung deflation the average values for the deflationary SAR adaptation index (AI) were below 40%. Intravenous administration of veratridine (50 microg/kg), an Na(+) channel opener, stimulated deflationary SAR activity: one maintained excitatory activity mainly during deflation and the other receptors showed a tonic discharge during both deflation and inflation. Despite the difference in deflationary SAR firing patterns after veratridine administration, flecainide treatment (6.0 mg/kg) blocked veratridine-induced deflationary SAR stimulation and also caused strong inhibition of the excitatory responses of deflationary SARs to lung deflation. Under these conditions, the average values for deflationary SAR AI were over 90%. The responses of deflationary SARs and deflationary SAR AI to lung deflation were not significantly altered by pretreatment with either 4-AP (0.7 and 2.0 mg/kg) or TEA (2.0 and 6.0 mg/kg). These results suggest that the excitatory effect of lung deflation on deflationary SAR activity is mediated by the activation of flecainide-sensitive Na(+) channels on the nerve terminals of deflationary SARs. 相似文献
5.
S Saiki N Meguro T Morita Y Tomooka O Maeda T Kinouchi M Kuroda T Miki M Usami T Kotake 《Nihon Hinyōkika Gakkai zasshi. The japanese journal of urology》1990,81(10):1537-1542
Two hybridomas secreting two monoclonal antibodies IgG1 B1.4 and IgG2a B1.6 were obtained by immunizing BALB/c mice with human bladder cancer cell line EJ-1. In immunohistochemical staining of cryopreserved tissues, B1.4 reacted with 0 of 9 grade 1 TCC, 6 of 11 grade 2, all of 6 grade 3 and five metastatic specimens. The antigen recognized by B1.4 was not expressed by normal urothelial cells but were expressed by vascular endothelial cells and muscle of tunica media. The target antigen of B1.6 was expressed by normal urothelial cells and all grade of TCC. In this study, it was demonstrated that poorly differentiated bladder cancer and metastatic specimens of bladder cancer express a vascular carbohydrate antigen. Taking the escape mechanism of immune surveillance, into consideration, it is possible that the antigen recognized by B1.4 is an indicator of metastatic potential of bladder cancer. 相似文献
6.
HERMAN TOURNAYE RONNY JANSSENS PAUL DEVROEY RK VAN STEIRTEGHEM 《International journal of andrology》1994,17(1):1-8
In order to evaluate the effects of pentoxifylline on sperm motility and longevity, a controlled in-vitro study was conducted on normozoospermic donor semen samples using the Cellsoft automated system for sperm motility analysis. After incubation and selection, pentoxifylline was found to improve the recovery of spermatozoa and to increase their velocity. In the subgroup of progressively motile spermatozoa, curvilinear velocity was also enhanced. It is concluded that pentoxifylline has an effect on the vigour, but not on the pattern, of sperm motion. Pentoxifylline did not improve the motility characteristics of senescent spermatozoa in normozoospermic sperm samples. Sperm survival, as shown by supra-vital staining, and motility longevity both decreased with time after pentoxifylline treatment. 相似文献
7.
V. JNSSON H. D. SCHRDER W. TROJABORG T. STAEHELIN JENSEN E. HIPPE M. MRK HANSEN 《Journal of internal medicine》1992,232(2):185-191
A study of 17 patients with autoimmune axonal or demyelinating peripheral neuropathy in combination with M-component is described. The M-component was associated with MGUS (monoclonal gammopathy of undetermined significance) in 12 patients, CLL in one patient, WaldenstrÖm's disease in one patient, and myeloma in three patients. Immunohistological examination with direct and indirect fluorescence showed binding of antibodies to nerve structures of the same class and light chain as seen in the M-component. In five cases of IgM M-component, the demyelinating neuropathy was caused by binding of the IgM M-protein and complement C3b to myelin-associated glycoproteins (MAG). In 12 cases with axonal neuropathy, binding of IgG to the connective tissue of the peri- and endoneurium was found in 50% of cases, IgM in five cases, and IgD in one case. None of the patients had central nervous system (CNS) symptoms. The clinical and therapeutic difficulties are discussed; only two patients with an acute course responded to immunosuppression. A marked co-expression of other autoimmune phenomena is interpreted in the light of cross-reactions between the autoantibody and similar tissue autoantigens. 相似文献
8.
9.
Ikuo Saiki Jun Murataxd Junya Yoneda Hideo Kobayashi Ichiro Azuma 《International journal of cancer. Journal international du cancer》1994,56(6):867-873
We have examined the influence of fibroblasts on the invasive and migratory potential of highly metastatic melanoma B16-BL6 and weakly metastatic B16-FI cells in vitro. Co-culture of B16-BL6 cells with a fibroblast monolayer without cellular contact in a Transwell chamber more effectively induced tumor-cell invasion into Matrigel basement membrane than co-culture of B16-FI cells with a fibroblast monolayer. The activity was closely correlated with the chemotactic migration of tumor cells toward the fibroblast monolayer. We also found that the conditioned medium (CM) from the co-culture of fibroblasts with B16-BL6 cells without cellular contact, i.e., CM (B16-BL6/fibroblast), rather than from co-culture with B16-FI cells, could potentially promote the migration of tumor cells of both types. Tumor cells did not chemotactically migrate to the CM (B16-BL6), CM (B16-FI) or CM (fibroblast). Antibodies against TGF-β1 or FN almost completely abolished the chemotactic migration of B16-BL6 cells to the CM (B16-BL6/fibroblast) or CM (TGF-β1 -treated fibroblast) when these antibodies were c-incubated with fibroblasts and either B16-BL6 or TGF-β1. In contrast, the anti-EGF antibody did not show any inhibitory effects. Analysis of amounts of TGF-β1 or FN in various CM using ELISA plates, and using their specific antibodies, revealed that the concentration of TGF-β1 in the CM (B16-BL6) was slightly higher than in the CM (B16-FI), and the amount of FN in the CM (B16-BL6/fibroblast) was twice as high as in the CM (B16-FI /fibroblast). These results suggest that TGF-β1 released from B16-BL6 cells can stimulate fibroblasts to produce FN; consequently, the tumor cells were able to chemotactically migrate toward the released FN, and the differences in invasive and migratory activities towards fibroblasts in B16-BL6 and B16-FI cells may in part be due to the amounts of TGF-β1 from tumor cells and of FN from TGF-β1 -stimulated fibroblasts. 相似文献
10.