全文获取类型
收费全文 | 1549篇 |
免费 | 189篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 52篇 |
儿科学 | 65篇 |
妇产科学 | 21篇 |
基础医学 | 243篇 |
口腔科学 | 15篇 |
临床医学 | 192篇 |
内科学 | 323篇 |
皮肤病学 | 10篇 |
神经病学 | 117篇 |
特种医学 | 76篇 |
外科学 | 257篇 |
综合类 | 85篇 |
预防医学 | 116篇 |
眼科学 | 31篇 |
药学 | 72篇 |
中国医学 | 1篇 |
肿瘤学 | 68篇 |
出版年
2022年 | 12篇 |
2021年 | 15篇 |
2020年 | 15篇 |
2019年 | 19篇 |
2018年 | 19篇 |
2017年 | 22篇 |
2016年 | 18篇 |
2015年 | 20篇 |
2014年 | 32篇 |
2013年 | 42篇 |
2012年 | 79篇 |
2011年 | 70篇 |
2010年 | 39篇 |
2009年 | 61篇 |
2008年 | 79篇 |
2007年 | 98篇 |
2006年 | 59篇 |
2005年 | 63篇 |
2004年 | 86篇 |
2003年 | 85篇 |
2002年 | 75篇 |
2001年 | 44篇 |
2000年 | 54篇 |
1999年 | 47篇 |
1998年 | 26篇 |
1997年 | 24篇 |
1996年 | 21篇 |
1995年 | 21篇 |
1994年 | 16篇 |
1993年 | 14篇 |
1992年 | 22篇 |
1991年 | 31篇 |
1990年 | 25篇 |
1989年 | 20篇 |
1988年 | 18篇 |
1987年 | 36篇 |
1986年 | 18篇 |
1985年 | 29篇 |
1984年 | 18篇 |
1983年 | 19篇 |
1982年 | 10篇 |
1979年 | 17篇 |
1978年 | 10篇 |
1977年 | 15篇 |
1976年 | 9篇 |
1974年 | 16篇 |
1970年 | 26篇 |
1969年 | 21篇 |
1968年 | 18篇 |
1967年 | 11篇 |
排序方式: 共有1744条查询结果,搜索用时 15 毫秒
1.
Although bladder function is thought to be unaffected in Duchenne muscular dystrophy, 46/88 boys interviewed had urinary problems. Nine underwent video urodynamics, showing in eight a small capacity, hyperreflexic bladder, and in the ninth (post spinal surgery) hyperreflexia and detrusor sphincter dyssynergia. Urinary dysfunction is a treatable feature of DMD. 相似文献
2.
The acromioclavicular joint is a potential source of pain in the shoulder. There are a variety of disorders that can affect this joint, including distal clavicle osteolysis, posttraumatic arthritis, osteoarthritis, and rheumatoid arthritis. Nonoperative treatment for this condition with nonsteroidal medication and activity modification can alleviate the pain. When conservative treatment is exhausted, surgical resection of the distal clavicle is often necessary. Arthroscopic resection of the distal clavicle preserves the acromioclavicular ligaments to prevent postoperative distal clavicle instability. The procedure is performed in either the beach chair or lateral position and requires the use of a shaver, electrocautery, and a burr for soft tissue and debridement and bone resection. 相似文献
3.
Debra L Ellies Beth Viviano John McCarthy Jean-Philippe Rey Nobue Itasaki Scott Saunders Robb Krumlauf 《Journal of bone and mineral research》2006,21(11):1738-1749
We compared and contrasted the mechanism of action for the cysteine knot protein subfamily, Wise and Sost (Sclerostin). Our data suggest that functional interactions between Sost or Wise and LRP5/LRP6 have the potential to regulate bone deposition by modulating the Wnt pathway. INTRODUCTION: The human disease sclerosteosis exhibits an increase in bone mass thought to be caused by hyperactive osteoblasts. Sclerostin, SOST, the gene affected in this disease, has been postulated to exert its activity by functioning as a BMP antagonist. However, recent evidence indicates that SOST is highly related to Wise, which can also modulate the Wnt pathway by binding to LRP5 and LRP6. MATERIALS AND METHODS: For this study, we used cell culture to test the BMP and Wnt activity function of both Wise and Sost. In addition, we used Xenopus in vivo Wnt assays along with Xenopus in vitro Wnt assays to support our cell culture results. Epitope tagged cell supernatants containing either Sost or soluble mutant or wildtype LRP5/LRP6 were used for immunoprecipitation. Sost immunoprecipitation results were confirmed in vivo using cell culture. Finally, to support our in vitro data, we co-localized Sost, Wise, LRP5, and LRP6 in mouse long bone sections. Results: In this study, we report in vitro and in vivo evidence to show that Sost physically interacts with Lrp5 and Lrp6 and inhibits the canonical Wnt signaling pathway. Furthermore, using in vitro and in vivo assays, we showed that a variant of LRP5 (LRP5(G171V)) known to cause the human high bone mass (HBM) trait and a homologous change in LRP6 (LRP6(G158V)) abolished protein interactions with Sost. We used variants of Sost amino acids to further identify the contact points between Sost and LRP6. In Xenopus and mammalian cell culture assays, we showed that SOST is able to attenuate Wnt signaling and that this attenuation can be rescued by the addition of alpha-Sost antibodies or by the introduction of single amino acid substitution that alter its binding to LRP6. Sost differs from Wise in that it is unable to stimulate Wnt signaling. Using immunohistochemistry, we found that Sost and Wise are co-localized to osteoblasts, along with LRP5 and LRP6. CONCLUSIONS: Our data suggest that functional interactions between Sost or Wise and LRPs have the potential to regulate bone deposition by modulating Wnt signaling. 相似文献
4.
5.
6.
S Plawecki E Carpentier P Lascombes J Prevot J E Robb 《Journal of pediatric orthopedics》1990,10(6):786-790
Three cases of congenital pseudarthrosis were treated by the Ilizarov method. Consolidation was obtained by 3.5 months in three patients who had undergone several previous surgical procedures. Although the length of follow-up was short (maximum 3.5 years), consolidation occurred more quickly than by traditional methods. This technique also permitted both simultaneous correction of axial malalignment and leg lengthening. We think that this method has a place as a salvage procedure except where the distal fragment is very thin or dystrophic. 相似文献
7.
Swartz MJ Batra SK Varshney GC Hollingsworth MA Yeo CJ Cameron JL Wilentz RE Hruban RH Argani P 《American journal of clinical pathology》2002,117(5):791-796
Pancreatic adenocarcinoma is believed to develop from histologically identifiable intraductal lesions known as pancreatic intraepithelial neoplasias (PanINs) that undergo a series of architectural, cytologic, and genetic changes, a progression model similar to the adenoma-carcinoma sequence in the colon. The apomucin MUC4 has been implicated in invasive pancreatic adenocarcinoma. MUC4 expression is not detectable at the RNA level in normal pancreas but is detectable at high levels in invasive pancreatic adenocarcinoma. We documented the pattern of expression of MUC4 in PanINs by studying a series of 71 PanIN lesions immunohistochemically using a new monoclonal antibody to MUC4. Five (17%) of 30 PanIN-1 lesions, 10 (36%) of 28 PanIN-2 lesions, 11 (85%) of 13 PanIN-3 lesions, and 25 (89%) of 28 invasive adenocarcinomas labeled with the MUC4 antibody used in the study. In addition, afew nonneoplastic lesions labeled with the MUC4 antibody, including reactive ducts in chronic pancreatitis, atrophic ducts filled with inspissated secretions, and ducts showing squamous metaplasia. Our data help establish the patterns of MUC4 expression in neoplastic precursors in the pancreas and add further support to the progression model for pancreatic adenocarcinoma. 相似文献
8.
Isolation of hybridoma cell lines and characterization of monoclonal antibodies against cholera enterotoxin and its subunits. 总被引:4,自引:5,他引:4 下载免费PDF全文
Hybridoma cell lines which produced monoclonal antibodies against cholera toxin were isolated. These cell lines were detected with an enzyme-linked immunosorbent assay screening procedure with purified cholera toxin or subunit A of cholera toxin. Seven cell lines were characterized with respect to their reactivity with cholera toxin subunits by Western blot analysis. Five clones produced antibodies which were directed against subunit A, and two clones produced antibodies which reacted with subunit B. These antibodies were also characterized by Western blot analysis for reactivity with the heat-labile enterotoxin produced by porcine and human enterotoxinogenic strains of Escherichia coli. Monoclonal antibodies which reacted with subunit A of cholera toxin also reacted with subunit A of both porcine and human heat-labile enterotoxins. In contrast, monoclonal antibodies to subunit B of cholera toxin did not react with subunit B of the heat-labile enterotoxin. Antibodies directed against subunit B neutralized the biological activity of cholera toxin in vitro in the S49 mouse lymphosarcoma assay. In contrast to polyclonal anti-subunit A antisera, monoclonal anti-subunit A from four of five clones had small but measurable neutralizing capacities in vitro. 相似文献
9.
10.
JE Robb LA Rymaszewski HB Bentley PT Donnan 《Surgical and radiologic anatomy : SRA》1991,13(3):181-185
Summary This study investigated the effect of tilt and observer reliability on radiographic measurements of the position of a prosthetic acetabular cup in seven dry bone pelves using the teardrop as a landmark. Coronal or sagittal tilt of more than five degrees was easily recognisable and there was effectively no observer variation in the measurements up to this limit. In addition, 90 out of 100 randomly selected antero-posterior pelvic radiographs from an outpatient department were not significantly rotated and 93 demonstrated a clearly defined teardrop. Measurements about the teardrop on routine radiographs are therefore sufficiently accurate to allow assessment of prosthetic position.
La valeur du sourcil cotyloidien comme repère d'analyse radiologique
Résumé Cette étude, conduite sur 7 bassins secs, apprécie l'effet de l'inclinaison du bassin sur la qualité de l'analyse radiographique de la position d'une cupule prothétique de hanche en utilisant le sourcil cotyloïdien comme repère. Une inclinaison du bassin dans les plans coronal et sagittal est aisément détectable et il n'existe pas de variation d'analyse entre les différents observateurs en dessous de 5° d'inclinaison. De plus, sur 100 radiographies antéro-postérieures de bassin choisies au hasard dans les dossiers de consultation, 90 avaient été réalisées sans incidence particulièrement adaptée et l'on pouvait repérer facilement le sourcil sur 93% d'entre elles. Les mesures faites sur des radios de routine sont donc suffisamment précises pour permettre l'évaluation de la position d'une prothèse à partir du sourcil cotyloïdien.相似文献