家兔肝的神经支配

本实验共用家兔17只,将20%的HRP溶液100μl,经肝门多点注入,其中10只家兔出现标记细胞.两侧T_(2-12)背根节内共270个,左侧占51.11±3.04%,右侧为48.89±3.04%,左、右侧差异无显著性(P>0.05).在T_(5-8)背根节内的标记细胞较多,占总数的59.26±2.99%,中、小型细胞共占94.53±1.60%.两侧结状神经节内的标记细胞共143个.左侧占53.15±4.17%,右侧占46.85±4.17%.两侧差异无显著性.中、小型细胞共占93.01±2.13%.两侧腹腔神经节内的标记细胞较多,计数其中2例,共582个,左侧占59.79±2.03%,右侧为40.21±2.03%.在迷走神经背核内仅发现2个标记细胞.     

Dengue determinants: Necessities and challenges for universal dengue vaccine development

Dengue illness can range from mild illness to life-threatening haemorrhage. It is an Aedes-borne infectious disease caused by the dengue virus, which has four serotypes. Each serotype acts as an independent infectious agent. The antibodies against one serotype confer homotypic immunity but temporary protection against heterotypic infection. Dengue has become a growing health concern for up to one third of the world's population. Currently, there is no potent anti-dengue medicine, and treatment for severe dengue relies on intravenous fluid management and pain medications. The burden of dengue dramatically increases despite advances in vector control measures. These factors underscore the need for a vaccine. Various dengue vaccine strategies have been demonstrated, that is, live attenuated vaccine, inactivated vaccine, DNA vaccine, subunit vaccine, and viral-vector vaccines, some of which are at the stage of clinical testing. Unfortunately, the forefront candidate vaccine is less than satisfactory, and its performance depends on serostatus and age factors. The lessons from clinical studies depicted ambiguity concerning the efficacy of dengue vaccine. Our study highlighted that viral structural heterogeneity, epitope accessibility, autoimmune complications, genetic variants, genetic diversities, antigen competition, virulence variation, host-pathogen specific interaction, antibody-dependent enhancement, cross-reactive immunity among Flaviviruses, and host-susceptibility determinants not only influence infection outcomes but also hampered successful vaccine development. This review integrates dengue determinants allocated necessities and challenges, which would provide insight for universal dengue vaccine development.     

Enhanced immune response to foot-and-mouth disease vaccine by oral administration of ginseng stem-leaf saponins

Vaccination is an important approach to the control of foot-and-mouth disease (FMD). This study evaluated the effect of oral administration of ginseng stem–leaf saponins (GSLS) on the immune response to FMD vaccine and the gut mucosal immunity in mice. In experiment 1, mice were orally administered GSLS or not treated as a control. The animals were then immunized twice with FMD vaccine. Blood was sampled weekly within five weeks after the boost immunization for measurement of serum IgG and the isotypes. In experiment 2, mice were orally administrated GSLS or not treated as a control. After that, splenocytes were prepared from sacrificed mice for lymphocyte proliferation assay and intestinal tissues were sampled for immunohistochemistry and histological examination. The results showed that oral administration of GSLS significantly enhanced serum IgG and the isotype responses to FMD vaccine as well as the number of intestinal intraepithelial lymphocytes (IELs) and immunoglobulin A (IgA)+?cells. Therefore, GSLS may be a potent oral adjuvant and deserve further study to improve vaccination in susceptible animals.     

急性CO中毒患者MRI MRA表现

目的评价MRI、MRA对急性CO中毒的诊断及价值。方法回顾分析60例经临床确诊的急性CO中毒患者的头颅MRI、MRA资料。结果MRI异常表现为双侧苍白球、双侧侧脑室周围、脑干、脑叶为主的T1WI高信号、T2WI低信号;18.33%急性CO中毒患者伴发脑梗死,DWI为高信号,ADC为低信号。结论急性CO中毒的诊断主要依靠病史、临床表现和影像检查,头颅MRI、MRA对本病的诊断有一定的价值。     

pH监测正常与异常的非糜烂性反流病患者高分辨率食管测压特征分析

目的应用高分辨率食管测压比较pH监测正常与异常非糜烂性反流病(NERD)患者的食管动力学特征。方法按标准选取具有典型胃食管反流症状的NERD患者35例和健康体检者10例,分别行上消化道内镜检查、24 h食管pH监测和高分辨率食管测压。依据动态pH监测Demeester评分将NERD患者分为pH监测异常组和pH监测正常组,比较3组患者食管动力的差异。结果 pH监测异常组食管下括约肌(LES)长度较对照组显著缩短(P<0.05),LES压力(LESP)显著降低(P<0.05),pH监测正常组与对照组比较则无明显差异。pH监测正常组和异常组均存在食管体部动力障碍,表现为食管远端收缩波波幅及远端收缩积分降低和有效蠕动比例减少,pH监测异常组改变更为明显。结论 pH监测不同NERD患者间存在食管动力特征差异,pH监测异常NERD组患者存在LES长度和压力异常,食管远端动力障碍更为明显。     

过氧化物酶体增殖物活化受体γ的抗氧化作用

过氧化物酶体增殖物活化受体(peroxisome proliferator activatived receptors,PPARs)是核受体超家族成员,包括PPARα、PPARδ和PPARγ3种亚型,他们在不同组织中表达,其中PPARγ在脂肪组织、心脏、 肠道和免疫细胞中存在,在脂肪合成过程中起着关键作用.PPARγ具...     

恩丹西酮联合地塞米松对腹腔镜手术后恶心呕吐的影响

目的观察恩丹西酮联合地塞米松对腹腔镜手术后恶心呕吐的影响。方法随机将100例在全麻下择期行腹腔镜手术的患者分为5组:A组,生理盐水对照组;B组,恩丹西酮组;C组,地塞米松组;D组,氟哌利多组;E组,恩丹西酮+地塞米松组。观察术后24h内病人恶心呕吐发生的情况及不良反应。结果A组恶心呕吐发生率为70%,明显高于B组(30%)、C组(30%)、D组(20%)和E组(15%),P<0.05;E组与B组和C组比较,P<0.05;与D组比较,P>0.05。但D组不良反应较多。结论恩丹西酮联合地塞米松静脉应用对降低腹腔镜手术后恶心呕吐的发生率有确切效果。     

TREATMENT OF 50 CASES OF MIGRAINE BY SCALP ACUPUNCTURE COMBINED WITH ELECTRO—COMB STIMULATION

Ｔｈｅａｕｔｈｏｒｓｏｆｔｈｅｐｒｅｓｅｎｔｐａｐｅｒｔｒｅａｔｅｄ 50ｃａｓｅｓｏｆｍｉｇｒａｉｎｅｂｙｓｃａｌｐａｃｕｐｕｎｃｔｕｒｅｐｌｕｓｅｌｅｃ ｔｒｏ ｃｏｍｂｓｔｉｍｕｌａｔｉｏｎｆｒｏｍ 1 995ｔｏ 1 999ａｎｄａｃｈｉｅｖｅｄｓａｔｉｓｆａｃｔｏｒｙｔｈｅｒａｐｅｕｔｉｃｅｆｆｅｃｔｓ.Ｉｔｉｓｒｅ ｐｏｒｔｅｄａｓｆｏｌｌｏｗｓ.1　ＣＬＩＮＩＣＡＬＤＡＴＡＥｉｇｈｔｙｏｕｔｐａｔｉｅｎｔｓｗｅｒｅｒａｎｄｏｍｌｙｄｉｖｉｄｅｄｉｎｔｏｓｃａｌｐａｃｕｐｕｎｃｔｕｒｅｐｌｕｓｅｌｅｃｔ…     

精神疾病患者住院费用分析

本文通过调查近5年来山东省精神卫生中心精神疾病患者的住院费用情况,了解其现状,探讨其影响因素,为政府制定相关精神卫生政策提供科学依据。     

山东省三地区彩民心理健康状况分析

目的了解山东省彩民的心理现状及影响因素。方法运用自制筛查量表和SCL-90作为工具对随机抽取的山东省三地区彩民进行调查研究,共计发放问卷1 160份,回收有效问卷1 142份;所有数据用SPSS11.5for windows统计软件包进行处理分析。结果山东省彩民有32.0%的人存在不同程度的心理健康问题,其中5.1%符合DSM-Ⅳ关于病理性赌博的标准。与全国常模比较,我省彩民SCL-90总分及大部分因子分较高(P<0.05);与同期正常对照人群相比,彩民SCL-90躯体化因子分、焦虑因子分、精神病性因子分显著高于对照组(P<0.05)。不同地区彩民的心理健康状况存在显著差异(P<0.05);已发展为病理性赌博彩民较普通彩民心理健康状况差,差异有统计学意义。以SCL-90总分为因变量,发大财、购彩失控、想翻本、整天只想与彩票有关的事情、做事情想立即见到成效、急需用钱、借钱购彩、因买彩票花钱过多与家人争吵、性格易冲动发怒等自变量进入Logistical回归方程。结论我省彩民32.0%存在心理问题,且存在显著的地区差异性;其中5.1%的彩民成为病理性赌博者。我省彩民心理问题主要表现在强迫、抑郁、人际关系敏感、焦虑等方面。发大财、购彩失控、想翻本、整天只想与彩票有关的事情、做事情想立即见到成效、急需用钱、借钱购彩、因买彩票花钱过多与家人争吵、容易冲动发怒等是影响彩民心理健康主要因素。