如何有效地提高护理专业生理学教学质量

通过在教学过程中不断改善教学方式,采用多种教学方法相结合的形式,有效地提高生理学教学质量.这样不仅可以为学生学习后续学科打下坚实的基础,而且提高了护理专业学生的临床应变能力.     

景颇族和汉族小学生同伴冲突应对与父母教养方式的关系

目的 探讨景颇族、汉族小学生同伴冲突应对方式和父母教养特点及相互关系.方法 采用小学生同伴冲突应对方式问卷和父母养育方式评价量表,对301名3～6年级景颇族和汉族小学生进行测查.结果 景颇族小学生的攻击[(2.45±0.78)分]、幻想[(2.71±0.70)分]分值均显著高于汉族,而退避分值[(2.49±0.61)分]明显低于汉族[(2.64±0.62)分];景颇族父母比汉族父母更多采用干涉、拒绝否认、偏爱等教养方式;父/母亲的温暖理解对子女问题解决、求助和退避等应对具有一定的预测作用,而母/父亲的偏爱可在一定程度上预测子女的攻击性应对.结论 小学生同伴冲突应对方式与父母教养方式密切关联,需要改进不良的家庭教养方式.     

间接喉镜下刮除术和鼻内镜下切吸术治疗腺样体肥大的手术效果比较

目的比较间接喉镜下腺样体刮除术和鼻内镜直视下腺样体切吸术治疗腺样体肥大的效果。方法118例患者分为A组(64例)和B组(54例),全身麻醉,用导尿管悬吊软腭,分别行间接喉镜下腺样体刮除术或鼻内镜直视下腺样体切吸术。结果所有患者术后打鼾症状明显改善,显效率分别为A组93.8%(60/64),B组94.4%(51/54);78例伴有渗出性中耳炎或复发性中耳炎,术后A、B组平均听阈提高分别为16±4.2dB和15±5.8dB;伴有鼻窦炎的患者,术后症状改善率分别为A组78.3%(18/23),B组88.9%(24/27)。术中平均出血量分别为A组20m1,B组25m1;平均手术时间分别为A组9分钟,B组7分钟。所有患者均无术后并发症发生。A组的手术费用明显低于B组。结论间接喉镜下腺样体刮除术和鼻内镜直视下腺样体切吸术均为安全、有效术式,但间接喉镜下腺样体刮除术简单易行、经济实惠。     

微种植体植入角度对微种植体稳定性的影响

目的:通过有限元方法,分析微种植体植入角度对微种植体稳定性的影响,为微种植体支抗的临床应用提供可靠的理论依据.方法:建立不同植入角度的微种植体-上颌全牙弓有限元模型,对各模型施加2.5N内收前牙的作用力,对微种植体所受等效应力进行比较.结果:微种植体与牙槽骨表面的夹角增加,微种植体所受等效应力明显减小.结论:微种植体植入角度的增加,有利于微种植体的稳定.     

野生型p53对纤维肉瘤端粒酶活性及Bcl-2、c-myc基因表达的影响

目的　观察野生型 p5 3 (Wtp5 3 )基因对纤维肉瘤端粒酶活性及B淋巴细胞 /白血病 2(Bcl 2 )、c myc基因表达的影响 ,探讨Wtp5 3抑瘤机制以及纤维肉瘤发病的分子机制。 方法　将 5周龄昆明鼠 90只随机分为单纯肿瘤组 (Ⅰ组 )和Wtp5 3基因注射组 (Ⅱ组 ) ,建立纤维肉瘤动物模型。 5d后 ,Ⅱ组动物于荷瘤局部注射介导Wtp5 3基因的腺病毒液 0 .1ml ,Ⅰ组注射同剂量生理盐水。两组动物于 2次注射后 5、15和 2 5d分批处死 ,聚合酶链反应 酶连免疫吸附 (PCR ELISA )法检测端粒酶活性 ,免疫组织化学法观察肉瘤细胞Bcl 2、c myc蛋白表达。结果　Ⅱ组中 ,端粒酶活性明显低于Ⅰ组 ,Bcl 2蛋白表达强度显著下调 ,c myc基因在 2次接种后 5d出现一过性低表达 ,而后表达强度上升。结论　Wtp5 3明显降低端粒酶活性 ,下调Bcl 2表达 ,改变c myc基因功能。从抑制肿瘤细胞增殖、促进细胞凋亡两方面发挥对纤维肉瘤的治疗作用。     

A SIMPLE EGG MEDIUM FOR THE CULTivATION OF BACILLUS DIPHTHERIAE

In 1884 Loeffler was able to isolate diphtheria bacilliin pure cul- tures from cases of dipbtheria using a medium consisting of a mixture of serum and glucose broth coagulated by heat. Thjq medium is now known as the Loeffler''s serum medium. Repeatedinvestigations have proven that this medium is the optimum medium for the cultivation of diphtheria bacilli and it has been almost univcrsally adopted for diagnostic purposcs.     

Purification and subunit structure studies of human placental threonyl-tRNA synthetase

Human threonyl-tRNA synthetase has been purified from full-term placenta over 5000-fold with a 13% overall yield by a combination of affinity chromatographic methods and conventional procedures. The product was apparently homogeneous by the criterion of sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The purified enzyme catalyzed the esterification of ? 3500 nmol L-threonine to tRNA per min per mg of protein at 37°, corresponding to a molecular activity of ? 700/min. The apparent molecular weight of the native enzyme ranged from 210 000 to 220 000 by gel-filtration on Sephadex G-200, and was either ? 110 000 or 228 000 by sucrose gradient centrifugation for different preparations. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis demonstrated a single band of molecular weight 85 000 or 115 000 for different preparations. These results suggest that human placental threonyl-tRNA synthetase has a subunit structure of the type α₂ with a subunit molecular weight of about 100 000. However, some other molecular forms with lower specific activity were also found to exist under certain conditions.