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1.
Janwan P Intapan PM Thanchomnang T Lulitanond V Anamnart W Maleewong W 《Parasitology research》2011,109(6):1593-1601
Human opisthorchiasis caused by the liver fluke Opisthorchis viverrini is an endemic disease in Southeast Asian countries including the Lao People’s Democratic Republic, Cambodia, Vietnam, and
Thailand. Infection with the soil-transmitted roundworm Strongyloides stercoralis is an important problem worldwide. In some areas, both parasitic infections are reported as co-infections. A duplex real-time
fluorescence resonance energy transfer (FRET) PCR merged with melting curve analysis was developed for the rapid detection
of O. viverrini and S. stercoralis in human fecal samples. Duplex real-time FRET PCR is based on fluorescence melting curve analysis of a hybrid of amplicons
generated from two genera of DNA elements: the 162 bp pOV-A6 DNA sequence specific to O. viverrini and the 244 bp 18S rRNA sequence specific to S. stercoralis, and two pairs of specific fluorophore-labeled probes. Both O. viverrini and S. stercoralis can be differentially detected in infected human fecal samples by this process through their different fluorescence channels
and melting temperatures. Detection limit of the method was as little as two O. viverrini eggs and four S. stercoralis larvae in 100 mg of fecal sample. The assay could distinguish the DNA of both parasites from the DNA of negative fecal samples
and fecal samples with other parasite materials, as well as from the DNA of human leukocytes and other control parasites.
The technique showed 100% sensitivity and specificity. The introduced duplex real-time FRET PCR can reduce labor time and
reagent costs and is not prone to carry over contamination. The method is important for simultaneous detection especially
in areas where both parasites overlap incidence and is useful as the screening tool in the returning travelers and immigrants
to industrialized countries where number of samples in the diagnostic units will become increasing. 相似文献
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Sadaow Lakkhana Sanpool Oranuch Phosuk Issarapong Rodpai Rutchanee Thanchomnang Tongjit Wijit Adulsak Anamnart Witthaya Laymanivong Sakhone Aung Win Pa Pa Janwan Penchom Maleewong Wanchai Intapan Pewpan M. 《Parasitology research》2018,117(8):2427-2436
Parasitology Research - Ascaris lumbricoides is the largest roundworm known from the human intestine while Ascaris suum is an internal parasite of pigs. Ascariasis, caused by Ascaris lumbricoides,... 相似文献
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Rodpai Rutchanee Intapan Pewpan M. Thanchomnang Tongjit Sanpool Oranuch Janwan Penchom Laummaunwai Porntip Wongkham Chaisiri Insawang Tonkla Maleewong Wanchai 《Parasitology research》2017,116(6):1687-1693
Parasitology Research - Strongyloides stercoralis is an intestinal helminth that infects people worldwide. Hyperinfection or disseminated human strongyloidiasis can involve vital organs, leading to... 相似文献
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Sanpool O Intapan PM Thanchomnang T Janwan P Lulitanond V Doanh PN Van Hien H Dung do T Maleewong W Nawa Y 《Parasitology research》2012,111(1):89-96
We developed a single step duplex real-time fluorescence resonance energy transfer (FRET) PCR merged with melting curve analysis for the fast detection and differentiation of Clonorchis sinensis and Opisthorchis viverrini eggs in human fecal samples. Two species of mitochondrial NADH dehydrogenase subunit 2 (nad2) DNA elements, the 165-bp nad2 product of C. sinensis and the 209-bp nad2 product of O. viverrini, were amplified by species-specific primers, and the fluorescence melting curve analyses were generated from hybrid of amplicons and two pairs of species-specific fluorophore-labeled probes. By their different fluorescence channels and melting temperatures, both C. sinensis and O. viverrini eggs in infected human fecal samples were detected and differentiated with high (100%) sensitivity and specificity. Detection limit was as little as a single C. sinensis egg and two O. viverrini eggs in 100 mg of fecal sample. The assay could distinguish the DNA of both parasites from the DNA of negative fecal samples and fecal samples with other parasitosis, as well as from the well-defined genomic DNA of human leukocytes and other parasites. It can reduce labor time of microscopic examination and is not prone to carry over contamination of agarose electrophoresis. Our duplex real-time FRET PCR method would be useful to determine the accurate range of endemic areas and/or to discover the co-endemic areas of two liver flukes, C. sinensis and O. viverrini, in Asia. This method also would be helpful for the differential diagnosis of the suspected cases of liver fluke infections among travelers who had visited the endemic countries of those parasites. 相似文献
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Lulitanond V Intapan PM Tantrawatpan C Sankuntaw N Sanpool O Janwan P Maleewong W 《Journal of clinical microbiology》2012,50(4):1455-1457
PCR amplification coupled with pyrosequencing was used to measure molecular markers that could be used to detect and differentiate Plasmodium falciparum and Plasmodium vivax in human blood samples. The detection rates were in agreement with the results of Giemsa-stained film microscopy, which is the current gold standard for detection. This method provides an exciting alternative for malaria diagnosis. 相似文献
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Jurairat Jongthawin Pewpan M. Intapan Oranuch Sanpool Lakkhana Sadaow Penchom Janwan Tongjit Thanchomnang Apichat Sangchan Siriraksa Visaetsilpanonta Worasak Keawkong Wanchai Maleewong 《The American journal of tropical medicine and hygiene》2015,93(3):615-618
Human gnathostomiasis is one of the important food-borne parasitic zoonoses. The disease is caused by a spirurid roundworm of the genus Gnathostoma. Here, we describe three parasitological confirmed cases of human gnathostomiasis, caused by Gnathostoma spinigerum, in a hospital in Thailand during 2004–2012. Clinical characteristics, treatment, and outcome of cases were revealed. Parasites were accidentally recovered from patients and morphologically identified as Gnathostoma species. Confirmed diagnosis and identification of causative parasite species was made by DNA extraction of the recovered worms, followed by a polymerase chain reaction (PCR) of the second internal transcribed spacer region (ITS2) of DNA and the partial cytochrome c oxidase subunit 1 (cox-1) gene. Sequences corresponding to ITS2 and cox-1 were similar to G. spinigerum. To our knowledge, this study represents the first molecular confirmation that recovered G. spinigerum is a causative agent of human infection in Thailand. 相似文献
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Anchalee Jintapattanakit Penchom Peungvicha Achariya Sailasuta Thomas Kissel Varaporn Buraphacheep Junyaprasert 《The Journal of pharmacy and pharmacology》2010,62(5):583-591
Objectives The objective of this work was to explore the potential and safety of trimethyl chitosan (TMC) and PEGylated TMC for improved absorption of insulin after nasal administration. Methods The nasal absorption of insulin nanocomplexes of TMC or PEGylated TMC was evaluated in anaesthetized rats. Concomitantly, the histopathological effects of these nanocomplexes on rat nasal mucosa were studied using a perfusion fixation technique. Key findings All insulin nanocomplexes containing TMC or PEGylated TMC showed a 34–47% reduction in the blood glucose concentration, when the insulin absorption through the rat nasal mucosa was measured indirectly. In addition, the relative pharmacodynamic bioavailability (Fdyn) of the formulations was found to be dependent upon the charge ratio of insulin and polymer, regardless of polymer structure. The Fdyn apparently decreased with increasing charge ratio of insulin : polymer. Although acute alterations in nasal morphology by the formulations were affected by the charge ratio of insulin and polymer, the formulation of insulin/PEGylated TMC nanocomplexes was shown to be less toxic to the nasal epithelial membrane than insulin/TMC nanocomplexes. Conclusions PEGylated TMC nanocomplexes were a suitable absorption enhancer for nasal delivery of insulin. 相似文献
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Yi Zhong Chutwadee Krisanapun Seong-Ho Lee Thararat Nualsanit Carl Sams Penchom Peungvicha Seung Joon Baek 《European journal of cancer (Oxford, England : 1990)》2010,46(18):3365-3374
Persuasive epidemiological and experimental evidence suggests that dietary flavonoids have anti-cancer activity. Since conventional therapeutic and surgical approaches have not been able to fully control the incidence and outcome of most cancer types, including colorectal neoplasia, there is an urgent need to develop alternative approaches for the management of cancer. We sought to develop the best flavonoids for the inhibition of cell growth, and apigenin (flavone) proved to be the most promising compound in colorectal cancer cell growth arrest. Subsequently, we found that pro-apoptotic proteins (NAG-1 and p53) and cell cycle inhibitor (p21) were induced in the presence of apigenin, and kinase pathways, including PKCδ and ataxia telangiectasia mutated (ATM), play an important role in activating these proteins. The data generated by in vitro experiments were confirmed in an animal study using APCMIN+ mice. Apigenin is able to reduce polyp numbers, accompanied by increasing p53 activation through phosphorylation in animal models. Our data suggest apparent beneficial effects of apigenin on colon cancer. 相似文献