Structure, Metabolism, and Functions of Peripheral Blood Lymphocytes during Long-Term Persistence of Epstein-Barr Virus

Study of surface architectonics, blast transformation potential, and cytochemical activity of peripheral blood lymphocytes from children infected with Epstein-Barr virus revealed imbalance between structural, metabolic, and functional state of lymphocytes. This imbalance persists in delayed period after infection and determines long-term viral persistence in the macroorganism.     

Role of cell signaling systems in the regulation of Ca2+-activated potassium channels in erythrocytes

The effect of inhibitors of calmodulin and protein kinase C, complex [AlF₄]⁻, and potassium channel blockers on the parameters of calcium-induced hyperpolarization in human erythrocytes is studied by recording proton distribution between the cytoplasm and incubation medium in the presence of a protonophore. It is shown that the calmodulin inhibitor dis-C₃-5, the protein kinase C inhibitor staurosporine, and the blocker of highconductance calcium-activated potassium channels considerably suppress the calciuminduced hyperpolarization in erythrocytes. High concentration of [AlF₄]⁻ in the incubation medium inhibits hyperpolarization. Translated fromByulleten' Eksperimental'noi Biologii i Meditsiny, Vol. 123, No. 6, pp. 653–655, June, 1997     

Effect of recombinant interleukin-5, interleukin-3, and eotaxin on apoptosis in eosinophilic granulocytes

We studied in vitro effects of recombinant interleukin-5, interleukin-3, and eotaxin on programmed death of eosinophils from healthy donors and patients with non-Hodgkin’s lymphomas associated with severe blood eosinophilia. Interleukin-5 and eotaxin produced the most potent antiapoptotic effect on eosinophils from healthy donors. In patients with non-Hodgkin’s lymphomas, spontaneous apoptosis in eosinophilic leukocytes was low and remained unchanged during incubation with recombinant proteins. __________ Translated from Byulleten’ Eksperimental’noi Biologii i Meditsiny, Vol. 143, No. 4, pp. 370–373, April, 2007     

One-Year Follow-up of Duodenal Ulcers after 1-Wk Triple Therapy for Helicobacter pylori

Objective : to study the ulcer recurrence rate of Helicobacter pylori-positive duodenal ulcers at 1 yr after eradication of the bacteria by triple therapy. Method : Patients with H. pylori-positive duodenal ulcers were randomized to receive either triple therapy for 1 wk plus omeprazole for 4 wk (THple+OMP) (n = 78), or omeprazole alone (OMP) for 4 wk (N = 77). Patients were followed up every 3 months for symptom enquiry. At 1 yr, all asymptomatic patients were invited to attend for gastroscopy. Results : At 8 wk, 16 patients in the OMP group and four in the Triple+OMP group had an ulcer. During the 1-yr period, 12 patients in the OMP group and no patient in the Triple+OMP group developed symptomatic ulcers. At follow-up endoscopy at 1 yr, another 10 ulcers were detected in the OMP group and two in the Triple+OMP group. Fifteen patients in the OMP group and 13 in the Triple+OMP group were lost to follow-up. In total, ulcers were de-tected in 39 of 61 (64%) assessahle patients in the OMP group, and in six of 65 (97o) assessahle patients in the Triple+OMP group after I yr (χ² test: p < 0.001). Of the patients whose H, pytori were successfully eradicated hy Triple+OMP at 8 wk, 90% remained H. pylori negative at 1 yr. Conclusion : Triple therapy for 1 wk eradicates H, pylori infection and significantly reduces duodenal ulcer relapses.