全文获取类型
收费全文 | 1642篇 |
免费 | 105篇 |
国内免费 | 25篇 |
专业分类
耳鼻咽喉 | 20篇 |
儿科学 | 79篇 |
妇产科学 | 38篇 |
基础医学 | 180篇 |
口腔科学 | 45篇 |
临床医学 | 191篇 |
内科学 | 288篇 |
皮肤病学 | 40篇 |
神经病学 | 106篇 |
特种医学 | 143篇 |
外科学 | 402篇 |
综合类 | 40篇 |
预防医学 | 82篇 |
眼科学 | 5篇 |
药学 | 61篇 |
中国医学 | 2篇 |
肿瘤学 | 50篇 |
出版年
2023年 | 7篇 |
2022年 | 13篇 |
2021年 | 25篇 |
2020年 | 25篇 |
2019年 | 35篇 |
2018年 | 39篇 |
2017年 | 31篇 |
2016年 | 28篇 |
2015年 | 48篇 |
2014年 | 79篇 |
2013年 | 85篇 |
2012年 | 86篇 |
2011年 | 66篇 |
2010年 | 71篇 |
2009年 | 72篇 |
2008年 | 54篇 |
2007年 | 75篇 |
2006年 | 61篇 |
2005年 | 74篇 |
2004年 | 42篇 |
2003年 | 48篇 |
2002年 | 57篇 |
2001年 | 29篇 |
2000年 | 46篇 |
1999年 | 35篇 |
1998年 | 49篇 |
1997年 | 44篇 |
1996年 | 46篇 |
1995年 | 28篇 |
1994年 | 35篇 |
1993年 | 32篇 |
1992年 | 21篇 |
1991年 | 19篇 |
1990年 | 19篇 |
1989年 | 24篇 |
1988年 | 22篇 |
1987年 | 19篇 |
1986年 | 15篇 |
1985年 | 18篇 |
1984年 | 11篇 |
1983年 | 18篇 |
1982年 | 15篇 |
1981年 | 12篇 |
1980年 | 15篇 |
1979年 | 5篇 |
1978年 | 10篇 |
1977年 | 15篇 |
1976年 | 11篇 |
1975年 | 8篇 |
1962年 | 5篇 |
排序方式: 共有1772条查询结果,搜索用时 48 毫秒
1.
Anatoly E Martynyuk Ling-Sha Ju Timothy E Morey Jia-Qiang Zhang 《World Journal of Psychiatry》2020,10(5):81-94
The progress of modern medicine would be impossible without the use of general anesthetics (GAs). Despite advancements in refining anesthesia approaches, the effects of GAs are not fully reversible upon GA withdrawal. Neurocognitive deficiencies attributed to GA exposure may persist in neonates or endure for weeks to years in the elderly. Human studies on the mechanisms of the long-term adverse effects of GAs are needed to improve the safety of general anesthesia but they are hampered not only by ethical limitations specific to human research, but also by a lack of specific biological markers that can be used in human studies to safely and objectively study such effects. The latter can primarily be attributed to an insufficient understanding of the full range of the biological effects induced by GAs and the molecular mechanisms mediating such effects even in rodents, which are far more extensively studied than any other species. Our most recent experimental findings in rodents suggest that GAs may adversely affect many more people than is currently anticipated. Specifically, we have shown that anesthesia with the commonly used GA sevoflurane induces in exposed animals not only neuroendocrine abnormalities (somatic effects), but also epigenetic reprogramming of germ cells (germ cell effects). The latter may pass the neurobehavioral effects of parental sevoflurane exposure to the offspring, who may be affected even at levels of anesthesia that are not harmful to the exposed parents. The large number of patients who require general anesthesia, the even larger number of their future unexposed offspring whose health may be affected, and a growing number of neurodevelopmental disorders of unknown etiology underscore the translational importance of investigating the intergenerational effects of GAs. In this mini review, we discuss emerging experimental findings on neuroendocrine, epigenetic, and intergenerational effects of GAs. 相似文献
2.
3.
4.
GP SCHWAB AL BLUM E BODNER B DALLEMAGNE K GLASER H KOOP F PACE W RÖSCH JR SIEWERT G WETSCHER 《Journal of gastroenterology and hepatology》1997,12(12):785-789
Gastroesophageal reflux disease (GERD) is the most common disease of the upper gastrointestinal tract. With the introduction of proton pump inhibitors medical treatment of GERD has been significantly improved. However, the development of laparoscopic antireflux surgery resulted in an increasing interest of surgeons in this disease. An interactive meeting was organized in order to develop an agreement between gastoenterologists and surgeons regarding therapeutic decisions and this is the main topic of this paper. 相似文献
5.
K A Fleming M Evans K C Ryley D Franklin R H Lovell-Badge A L Morey 《The Journal of pathology》1992,167(1):9-17
The sensitivity of non-isotopic in situ hybridization (NISH), particularly on formalin-fixed, paraffin-embedded (FFPE) clinical tissues, has been the subject of controversy. Generally, NISH has been regarded as being less sensitive than radiolabelled procedures, although some reports have contradicted this. Accordingly, tissues from mice which were transgenic for variable amounts of the human alpha-1-antitrypsin gene were used to optimize the NISH procedure and to estimate the sensitivity. This approach showed that prolonged incubation of slides in final substrate resulted in high sensitivity--about 13 kb of target DNA. However, this prolonged incubation crucially depended on achieving minimal non-specific background staining. Many factors affected the degree of background staining, but five were particularly important. First, the method of mounting cut sections onto slides. Second, the length of the probe (ideally less than 400 bp). Third, the procedure for proteolytic digestion. Fourth, the denaturation technique, and fifth, the quality of the dextran sulphate used in the hybridization mix. The optimized protocol showed variable patterns of mRNA distribution in the transgenic mouse livers, while DNA distribution appeared uniform. 相似文献
6.
7.
8.
9.
Edward?J?HolloxEmail author Jane?Davies Uta?Griesenbach Juliana?Burgess Eric?WFW?Alton John?AL?Armour 《Journal of negative results in biomedicine》2005,4(1):9
Human beta-defensin 2 (DEFB4, also known as DEFB2 or hBD-2) is a salt-sensitive antimicrobial protein that is expressed in
lung epithelia. Previous work has shown that it is encoded in a cluster of beta-defensin genes at 8p23.1, which varies in
copy number between 2 and 12 in different individuals. We determined the copy number of this locus in 355 patients with cystic
fibrosis (CF), and tested for correlation between beta-defensin cluster genomic copy number and lung disease associated with
CF. No significant association was found. 相似文献
10.
Missense FGFR3 mutations create cysteine residues in thanatophoric dwarfism type I (TD1) 总被引:10,自引:1,他引:10
Rousseau F; el Ghouzzi V; Delezoide AL; Legeai-Mallet L; Le Merrer M; Munnich A; Bonaventure J 《Human molecular genetics》1996,5(4):509-512
Thanatophoric dwarfism (TD) is a sporadic lethal skeletal dysplasia with
micromelic shortening of the limbs, macrocephaly, platyspondyly and reduced
thoracic cavity. In the most common subtype (TD1), femurs are curved, while
in TD2, straight femurs are associated with cloverleaf skull. Mutations in
the fibroblast growth factor receptor 3 (FGFR3) gene were identified in
both subtypes. While TD2 was accounted for by a single recurrent mutation
in the tyrosine kinase 2 domain, TD1 resulted from either stop codon
mutations or missense mutations in the extracellular domain of the gene.
Here, we report the identification of FGFR3 mutations in 25/26 TD cases.
Two novel missense mutations (Y373C and G370C) were detected in 8/26 and
1/26 TD1 cases respectively. Both mutations created cysteine residues in
the juxta extramembrane domain of the receptor. Sixteen cases carried the
previously reported R248C (9/26 cases), S249C (2/26 cases) or stop codon
FGFR3 mutations (5/26 cases). Our results suggest that TD1 is a genetically
homogeneous condition and give additional support to the view that newly
created cysteine residues in the extracellular domain of the protein play a
key role in the severity of the disease.
相似文献