体外血栓形成机理的实验研究

血栓形成与流体力学因素关系密切，用健康成人血样进行的实验表明，Chandler血栓环内血液呈现出三维流动和二次流动状态，在管壁处的剪切应力最大。体外血栓形成应考虑血栓环弯曲和内部血液回流因素。血栓环内下弯月面处血栓的形成可能是流速相对较高的流体元冲击下弯月面导致血小板和红细胞聚集所至。当血栓环旋转时间低于10min时，血栓形成不良。15min是血栓形成的最佳时间。转速为3．75rpm时，不能形成血栓。     

Characterisation of the IgE-binding immunodominant epitopes on Ara h1

Peanut allergy is one of the most severe food allergies due to its persistency and life-threatening character. Serum IgE from patients with documented peanut hypersensitivity reactions and synthetic peptides were used to screen the linear IgE-binding epitopes on the major peanut allergen, Ara h 1. Five major epitopes that bound peanut-specific serum IgE from more than 60% of patients tested were identified. Mutational analysis of the immunodominant epitope showed that single amino acid changes had dramatic effects on IgE-binding characteristics. Mapping and characterisation of the IgE-binding epitopes on Ara h1 could be used in future immunotherapeutic approaches for peanut allergy disease.     

人工全髋关节置换术后关节腔内引流的临床观察

Clinical observation of drainage in articular chamber after total hip arthroplasty (TAH)人工全髋关节置换术,关节腔内引流Key words drainage,artificial jiont,total hip arthroplasty,R687.4目的:通过对引流量、创口愈合及引流液细菌培养的观察分析,确定最佳引流时间。方法:78例手术均放置关节腔引流,分别记录12h、24h、48h的引流量,并根据引流量情况分为24h和48h拔管两组,记录两组创口愈合、引流管出口处渗血情况,分别对三个时段的引流液及引流管体内端进行细菌培养,然后,分析各项数据。结果:平均引流量:12h 289ml,24h 414ml;48h 520ml;12/24h引流量比为69.8%。24/48h引流量比为79.6%。引流量主要发生在24h之内。78例髋术后未发生过深部感染。引流口均自行闭合,大多数未出现明显渗血及渗液现象。留取的12h、24h、48h引流液培养均未见细菌生长;引流末端培养亦未见细菌生长。结论:我们认为对12~24h引流量超过200ml的病例,应再留置引流管12~24h,以免出现引流管口渗血、延迟愈合情况的发生。     

四肢骨肉瘤保肢治疗与预后影响因素分析

目的 探究四肢骨肉瘤保肢治疗与预后的危险因素.方法 89例四肢骨肉瘤患者行保肢治疗后随访3年,依据3年预后结局分为生存组(71例)与死亡组(18例),比较两组患者临床特征,分析死亡的相关因素,将相关因素代入Cox比例风险模型分析危险因素.结果 生存组及死亡组四肢骨肉瘤患者Enneking分期及是否术后肺转移、治疗后病理...     

经皮椎体成形术治疗骨质疏松脊柱压缩性骨折疗效及并发症分析

目的分析经皮椎体成形术治疗骨质疏松脊柱骨折疗效及并发症。方法98例骨质疏松压缩性骨折患者随机分为PVP组50例和PKP组48例,分别采用经皮椎体成形术和经皮椎体后凸成形术进行治疗,比较和分析两组疗效及并发症。结果PVP组单椎手术时间、骨水泥注入量均较PKP组少（P〈0．05）;PKP组术后伤椎增加高度显著高于PVP组（P〈0．05）;两组手术前后疼痛均显著缓解,无显著差异（P〉0．05）;术后两组主要并发症均为骨水泥渗漏,无严重并发症。结论PVP是一种手术时间短、止痛效果好的骨质疏松脊柱压缩性骨折微创治疗手段,椎体高度均得到不同程度恢复,且稳定性较好,患者术后恢复快,生活质量明显提高。     

中医医联体学科一体化建设

医联体作为优化医疗资源配置的重要手段,在深化公立医院改革中发挥重要作用.北京中医药大学东直门医院中医医联体,探索以学科一体化为引导的多层面医联体建设,提出自己的建设方法和思考,希望为我国中医医联体发展提供借鉴.     

淋巴细胞转移抗病毒活性的机理研究

本文研究了人淋巴细胞与FL细胞共同培养后干扰素介导的抗病毒活性转移的机理。受者FL细胞表达的抗病毒转移活性具有下述特点:①用PHA封闭FL细胞膜上的干扰素受体后,不影响FL细胞表达转移的抗病毒活性;②混合培养时存在的抗2-5P_3A_3抗体不影响FL细胞表达抗病毒活性;③用放线菌素D抑制FL细胞的mRNA合成,能抑制FL细胞表达抗病毒活性。据此我们认为,干扰素活化后淋巴细胞膜上形成的第二信使分子可能是介导细胞之间密切接触转移抗病毒活性的重要介质。     

Synthetic magnetic resonance imaging for quantitative parameter evaluation of temporomandibular joint disorders

Objective:This study investigated the usefulness of quantitative parameters [longitudinal relaxation (T1), transverse relaxation (T2), and proton density (PD)] obtained with synthetic magnetic resonance imaging (MRI) in assessing the progression of temporomandibular joint (TMJ) disorders.Methods:For individual TMJ disorder diagnoses, the presence of disc displacement in MRI and the osseous change in cone-beam CT were investigated. Joints were classified into three stages: (1) silent stage, no disc displacement or osseous change; (2) incipient stage, presence of disc displacement and absence of osseous change; and (3) progressed stage, both disc displacement and osseous change. In synthetic MRI, the T1, T2, and PD values of the condyle bone marrow were measured simultaneously. The median T1, T2, and PD values were analyzed according to disc displacement, osseous changes, and joint stage.Results:Significant differences were observed in the T1 and PD values of joints with disc displacement or condylar osseous change compared to normal joints. The T1 and PD values also differed between the silent and progressed stages. The PD value differed between the silent and incipient groups, while the T2 value did not differ significantly among the three groups.Conclusion:The PD and T1 values of condylar bone marrow obtained from synthetic MRI can be used as sensitive indicators of TMJ disorder progression. The PD value of the bone marrow showed potential as a useful biomarker for recognizing the initial stages of TMJ disorders. Synthetic MRI is useful for the simultaneous acquisition of effective MRI parameters for evaluating TMJ disorders.     

结直肠癌患者血清肿瘤标志物联合检测的临床意义

目的探讨血清肿瘤标志物联合检测在结直肠癌(CRC)患者病情监测中的临床意义。方法 116例CRC患者根据不同肿瘤分期分为Ⅰ~Ⅱ期组50例和Ⅲ~Ⅳ期组66例,比较2组血清肿瘤标志物水平。同时比较化疗后12个月和24个月时复发转移与未复发转移者血清肿瘤标志物水平差异。结果Ⅰ~Ⅱ期组患者血清癌胚抗原(CEA)、糖链抗原19-9(CA19-9)和CA242水平均显著低于Ⅲ~Ⅳ期组,差异有统计学意义(P0.05);2组血清甲胎蛋白(AFP)、CA724水平比较,差异无统计学意义(P0.05);化疗后12个月,复发转移与无复发转移患者血清CEA、CA199、CA242、CA724水平比较,差异有统计学意义(P0.05);2组血清AFP水平比较,差异无统计学意义(P0.05);化疗后24个月,复发转移与无复发转移患者血清CEA、CA199、CA242、AFP、CA724水平比较,差异有统计学意义(P0.05)。结论血清肿瘤标志物联合检测在评估CRC患者病情、监测化疗后是否复发转移上具有一定临床价值。