全文获取类型
收费全文 | 494篇 |
免费 | 36篇 |
国内免费 | 4篇 |
专业分类
耳鼻咽喉 | 6篇 |
儿科学 | 20篇 |
妇产科学 | 11篇 |
基础医学 | 48篇 |
口腔科学 | 6篇 |
临床医学 | 36篇 |
内科学 | 114篇 |
皮肤病学 | 5篇 |
神经病学 | 44篇 |
特种医学 | 34篇 |
外科学 | 95篇 |
综合类 | 10篇 |
预防医学 | 22篇 |
眼科学 | 4篇 |
药学 | 43篇 |
中国医学 | 5篇 |
肿瘤学 | 31篇 |
出版年
2023年 | 8篇 |
2022年 | 5篇 |
2021年 | 17篇 |
2020年 | 7篇 |
2019年 | 8篇 |
2018年 | 22篇 |
2017年 | 18篇 |
2016年 | 12篇 |
2015年 | 25篇 |
2014年 | 18篇 |
2013年 | 22篇 |
2012年 | 30篇 |
2011年 | 30篇 |
2010年 | 20篇 |
2009年 | 11篇 |
2008年 | 16篇 |
2007年 | 17篇 |
2006年 | 23篇 |
2005年 | 26篇 |
2004年 | 12篇 |
2003年 | 22篇 |
2002年 | 19篇 |
2001年 | 10篇 |
2000年 | 14篇 |
1999年 | 7篇 |
1998年 | 4篇 |
1997年 | 5篇 |
1996年 | 7篇 |
1995年 | 7篇 |
1993年 | 4篇 |
1992年 | 12篇 |
1991年 | 5篇 |
1990年 | 10篇 |
1989年 | 9篇 |
1988年 | 4篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1984年 | 4篇 |
1983年 | 4篇 |
1979年 | 2篇 |
1976年 | 4篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 4篇 |
1972年 | 2篇 |
1970年 | 3篇 |
1969年 | 4篇 |
1940年 | 1篇 |
1935年 | 1篇 |
1931年 | 1篇 |
排序方式: 共有534条查询结果,搜索用时 15 毫秒
1.
2.
3.
Paul Kruszka Tommy Hu Sungkook Hong Rebecca Signer Benjamin Cogné Betrand Isidor Sarah E. Mazzola Jacques C. Giltay Koen L. I. van Gassen Eleina M. England Lynn Pais Charlotte W. Ockeloen Pedro A. Sanchez‐Lara Esther Kinning Darius J. Adams Kayla Treat Wilfredo Torres‐Martinez Maria F. Bedeschi Maria Iascone Stephanie Blaney Oliver Bell Tiong Y. Tan Marie‐Ange Delrue Julie Jurgens Brenda J. Barry Elizabeth C. Engle Sarah K. Savage Nicole Fleischer Julian A. Martinez‐Agosto Kym Boycott Elaine H. Zackai Maximilian Muenke 《American journal of medical genetics. Part A》2019,179(10):2075-2082
Zinc finger protein 462 (ZNF462) is a relatively newly discovered vertebrate specific protein with known critical roles in embryonic development in animal models. Two case reports and a case series study have described the phenotype of 10 individuals with ZNF462 loss of function variants. Herein, we present 14 new individuals with loss of function variants to the previous studies to delineate the syndrome of loss of function in ZNF462. Collectively, these 24 individuals present with recurring phenotypes that define a multiple congenital anomaly syndrome. Most have some form of developmental delay (79%) and a minority has autism spectrum disorder (33%). Characteristic facial features include ptosis (83%), down slanting palpebral fissures (58%), exaggerated Cupid's bow/wide philtrum (54%), and arched eyebrows (50%). Metopic ridging or craniosynostosis was found in a third of study participants and feeding problems in half. Other phenotype characteristics include dysgenesis of the corpus callosum in 25% of individuals, hypotonia in half, and structural heart defects in 21%. Using facial analysis technology, a computer algorithm applying deep learning was able to accurately differentiate individuals with ZNF462 loss of function variants from individuals with Noonan syndrome and healthy controls. In summary, we describe a multiple congenital anomaly syndrome associated with haploinsufficiency of ZNF462 that has distinct clinical characteristics and facial features. 相似文献
4.
Expansion of cytotoxic CD8+ CD28- T cells in healthy ageing people, including centenarians. 总被引:5,自引:1,他引:5
下载免费PDF全文
![点击此处可从《Immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
F F Fagnoni R Vescovini M Mazzola G Bologna E Nigro G Lavagetto C Franceschi M Passeri P Sansoni 《Immunology》1996,88(4):501-507
Ageing is associated with complex remodelling in the phenotypic and functional profiles of T lymphocytes. We investigated whether expression of CD28 antigen on T cells is conserved throughout adulthood and ageing in humans. For this purpose we analysed T cells obtained from peripheral blood of 102 healthy people of ages ranging from 20 to 105 years. We found an age-related increase of CD28- T cells in percentage and absolute number, predominantly among CD8+ T cells. CD28- T cells from aged donors analysed by flow cytometry appeared as resting cells (not expressing CD25, CD38, CD69, CD71, DR), bearing markers of cytotoxic activity (CD 11b and CD 57) and with a phenotype compatible with 'memory' cells (up-regulated CD2 and CD11a; CD62L absent). At the functional level, freshly isolated purified CD28- CD8+ T cells showed high anti-CD3 redirected cytotoxic activity against Fc-bearing P815 cells. The same activity tested on freshly isolated bulk T lymphocytes was significantly augmented with age. We found a positive correlation between age, number of CD8+ CD28- T cells and anti-CD3 redirected cytotoxicity by freshly isolated T cells. These data suggest that an activation of unknown nature within the cytotoxic arm of the immune system occurs with age. We speculate that these cytotoxic T lymphocytes (CTL) in vivo may constitute armed effector cells for immediate killing of targets bearing peptides from pathogens of intracellular origin. 相似文献
5.
Ilaria Ferrarotti Michele Zorzetto Roberta Scabini Paola Mazzola Ilaria Campo Maurizio Luisetti 《Diagnostic molecular pathology》2004,13(3):160-163
There is worldwide growing awareness of alpha 1-antitrypsin deficiency (AATD), a major hereditary disorder in Caucasians. The gold standard for laboratory diagnosis of AATD is thin-layer isoelectrofocusing (IEF), which is labor intensive and should be performed in reference laboratories. The aim of this study was to find an easy, fast, and cheap method for detecting alpha1-antitrypsin S and Z variants, the most frequent variants associated with AATD. The novel method herein described is based on SexAI/Hpy99I RFLP. We studied samples from 90 subjects enrolled in the Italian National Registry for AATD, previously typed by isoelectrofocusing. We found a complete agreement among our results, IEF, and genotypes obtained by standard methods. We concluded that this novel method combines efficiency, ease, swiftness, and low cost. 相似文献
6.
C Castagnoli D Peruccio M Stella G Magliacani G Mazzola A Amoroso P Richiardi 《Human immunology》1990,29(3):229-232
Nineteen patients that had developed hypertrophic scars subsequent to thermal injury were typed for HLA class II allogenotypes with the restriction fragment length polymorphism technique. A significant association was found with DR beta 16 (pc = 1.45 x 10(-4); relative risk = 12.25). This finding adds evidence to other data suggesting that immunologic phenomena are involved in pathologic scarring. Moreover, the results presented here have allowed an identification of a genetically determined risk factor for hypertrophic scar formation located in the HLA region. 相似文献
7.
New HLA-A*11 allele,A*1112, identified by sequence-based typing 总被引:1,自引:0,他引:1
Garino E Belvedere M Berrino M Bertola L Dall'Omo AM Mazzola G Rossetto C Carcassi C Lai S Gay E Ricotti M Curtoni ES 《Tissue antigens》2002,60(1):84-87
In this report, we describe the identification of HLA-A*1112, a novel HLA-A*11 allele found in two Italian families. The new allele was detected during routine HLA typing by a polymerase chain reaction sequence-specific primer and was confirmed by high-resolution sequencing-based typing. The nucleotide sequences of HLA-A*1112 exons 2 and 3 are identical to HLA-A*11011 except for a single nucleotide substitution in codon 90 (GAC-->GCC). 相似文献
8.
Romano GC Potestio M Scialabba G Mazzola A Candore G Lio D Caruso C 《Mechanisms of ageing and development》2000,121(1-3):231-238
T cell function is altered in vivo and in vitro in elderly compared with young subjects, and this alteration is believed to contribute to morbidity and mortality in man due to the greater incidence of infection, as well as autoimmunity and cancer in elderly. The majority of T cells express TCRalphabeta whereas TCRgammadelta is expressed on a minority of T cells. Moreover, it is known that gammadelta T lymphocytes display major histocompatibility complex (MHC)- unrestricted cytotoxicity that is reminiscent of natural killer (NK) activity. In view of earlier findings on both T cells and NK cells in the elderly, we hypothesised a different behaviour of gammadelta T lymphocytes from old subjects when compared with gammadelta T lymphocytes obtained from young people. Therefore, to gain further insight into mechanisms of immunosenescence in this little-studied population, we studied immunofluorescence analysis gammadelta T cells from the elderly. Our preliminary results show that the percentage of blood gammadelta T cells in lymphocytes from old subjects is decreased when compared with the young. Interestingly, these cells are more activated in the elderly than in young subjects; expression of CD69, an early activation marker, is increased in gammadelta T lymphocytes from old subjects after three hours of in vitro culture both with and without lipopolysaccharide stimulation. Thus, our findings, which need confirmation, strongly suggest that, in humans, gammadelta T cells are early responders when compared with alphabeta T cells. They may act as 'first aid' cells to replace the described deficit of the specific and aspecific immunity in elderly. In this view, the proinflammatory status, observable in the elderly, renders them ready to be stimulated by exogenous agents. 相似文献
9.
Giulia Marvaso Marianna Montesano Giulia Corrao Simone P. De Angelis Sara Gandini Giovanni C. Mazzola Matteo Augugliaro Jessica Franzetti Dario Zerini Matteo Pepa Stefano Luzzago Francesco A. Mistretta Gennaro Musi Franco Nolè Ottavio De Cobelli Roberto Orecchia Barbara A. Jereczek-Fossa 《BJU international》2021,127(4):454-462
10.
Favre A Briano S Mazzola C Brizzolara A Torre M Cilli M Sanguineti M Martucciello G 《Journal of pediatric surgery》1999,34(12):1818-1821
BACKGROUND: The spontaneous mutant Danforth's short tail (Sd) mouse has been studied over the last 60 years from the morphological, embryological, and genetic point of view. The Sd mutation affects a gene essential to notochordal development, and the Sd mouse phenotype represents an analogue of human caudal regression syndrome. The Sd/Sd mouse presents different types of anorectal malformations (ARM) and was suggested as a simple and cheap model of investigation of ARM morphology and embryology. In the current study, the Sd mouse enteric nervous system (ENS) was thoroughly investigated with specific immunohistochemical markers. METHODS: Macroscopic analysis, normal histology, and immunohistochemical techniques for detecting neurofilaments (NF) and NOS1 were used to study ENS of 138 Sd mice and 25 controls. RESULTS: The surprising results of this study showed that Sd mutation is associated with different degrees of hypoganglionosis and aganglionosis. In 41% of Sd/SD-affected mice, the rectal pouch was aganglionic and in the remaining 58% was severely hypoganglionic. In addition, 4.1% of heterozygous mice presented a distal aganglionosis and 8.3% hypoganglionosis. CONCLUSIONS: These results suggest that Sd mutation independently affects distinct cell lines during early organogenesis, as notochord cells, ventral hingut endoderm, and neuroblasts migrating from neural crest cells. Comparing the Sd murine model with human pathology, this study confirms that the association between ARM and intestinal dysganglionosis is not rare and underlines the importance of detecting in every ARM patient the innervation abnormalities of rectal pouch and fistulas. 相似文献